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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc]
    #29197774 -

Huskies said:
oooh, that's a smooth technique.


Thanks
:bashful:
I flame sterilize, you can see @0:02 in the video very clearly how far down the handle I go with the torch - I start at the bottom and get the tip red hot.

I don't use much IPA and have a FFU to keep the air moving. I started to wear a mask too if my partner is around because they'll sometimes start talking to me and I don't want that to affect my transfers.



ReverendMyc said:
However, now I just take about 1 to 2 cm strip from the leading edge when it looks like strong regular growth. Someone here put the idea in my head that using the older growth and the transfer piece was less desirable. So far I have not regretted the change. Might not be strictly necessary, but I like the vigor as the new growth takes off on the grains.



Does this change the way you do your LI? I'm curious about your opinion on the video I just uploaded doing a LI transfer.

Edited by sage_bd (04/24/25 01:37 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sage_bd] * 1
    #29197807 -

I honestly do not understand why people have a hard time using a scalpel to cut and transfer agar.  It's a really simple process, just plan your moves and execute them smoothly and slowly. :shrug:  I have ptsd shakey hands and can do it.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy]
    #29197888 -

I agree for a single transfer using a scalpel is easy imo but doing many becomes a pain especially if the wedges are larger.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sage_bd] * 2
    #29197898 -

Genuinely love the video, buddy. I thought about asking if you minded if I linked to it (or made a short gif from it) to use in my LI tek. Nicely done.

I mostly use scalpels with a #7 or #12 blades, but find the different pimple poppers really convenient for various things and will often have all the options handy.

I just started leaving the oldest center area from the transfer on the plate for LIs and grain inoculations. But I don't know that it really makes a difference other than increasing my confidence a little.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 1
    #29197901 -

Ah thanks :heart: yeah you're welcome to repurpose it as you see fit - a short gif probably would work best - I actually just re-posted in your LI thread. But you're welcome to make a gif of it to re-post in the main post for visibility as I know things get buried in the comments after some time passes.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #29197907 -

Smellyhobbit said:
Thurman you need to get on SSLC.


Ive been using that quite a bit lately, actually. It's nice except Ive got so much going on agar now that my priorities shift and then Ive got 4 of my 5 bottles occupied by LC of stuff I dont even care about anymore. Must...get...more...bottles!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 2
    #29197941 -

I saw a post a few months back where somebody was using some kind of tool that cut perfectly  even circles into agar at the push of a button! Does anyone know the name of that tool? As I do more and more transfers, it is nice to get specialized tools to speed things up! :takingnotes:


--------------------
Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.

Cybesa's Ultimate woodlover project



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Re: AGAR ENVY! (Anything and All things agar!) [Re: cybesa] * 1
    #29197955 -

cybesa said:
I saw a post a few months back where somebody was using some kind of tool that cut perfectly  even circles into agar at the push of a button! Does anyone know the name of that tool? As I do more and more transfers, it is nice to get specialized tools to speed things up! :takingnotes:


It's an agar punch. I have one that isn't spring loaded - just a circular punch and then I pluck it with a scalpel and transfer. I dig it...


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 1
    #29197972 -

Read this quote here but "peace of mind is priceless.":D it makes sense to me too and i actually told myself before bed to do this (working around the t-fer piece) when knocking up jars today and totally forgot until now :facepalm3:, a touch upset but the plate looked so good i still feel okay:rofl:

Btw i miss your old sig. It had a huge impact on me when i started as well as your first journal, just had to mention that :grin:

Edited by nIceCube (04/24/25 05:52 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 1
    #29197987 -

Gotcha, cheers! How big are they? Do they fit in a HY-800 Bactericinerator? My scalpels fit perfectly, but I'm afraid the agar punches wouldn't if they are too big and relatively "bulky".. :strokebeard:

Here is what I use for sterilization of tools (HY-800)


It was probably the best investment next to the Flow Hood I ever made in the hobby. :grin:


--------------------
Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.

Cybesa's Ultimate woodlover project



Edited by cybesa (04/24/25 06:11 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 1
    #29198263 -

ThurmanMurman said:
cybesa said:
I saw a post a few months back where somebody was using some kind of tool that cut perfectly  even circles into agar at the push of a button! Does anyone know the name of that tool? As I do more and more transfers, it is nice to get specialized tools to speed things up! :takingnotes:


It's an agar punch. I have one that isn't spring loaded - just a circular punch and then I pluck it with a scalpel and transfer. I dig it...


Cybesa you can also look on Amazon for a leather punch set. For $16 I recently got a set that had twelve metal punches from sizes 1mm to 10mm. They work great. They had anodized aluminum agar punches on there for I think $18 each. They basically do the exact same thing.

https://a.co/d/b7b37Sk

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112]
    #29198267 -

I tried leather punches, but I felt like if I got it hot enough to sterilize, it might burn through the plate and the table before it slowed down. Too much mass. Or maybe it didn't need to be all the way glowing red.

:shrug:


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 2
    #29198279 -

Agar gone crazy!

I dropped this T1 plate of P Mexicana Galindoi a few days ago and I was upset because I thought I ruined it when the two transfers I put in there became loose and rolled around in the plate. I put two in the plate because I wanted to see what would happen if I put one upside down and the right side up. Looked and the plate today and I guess wherever the transfers touched, I now have mycelium growing. Pretty cool!




This first inoculation plate is Mazatapec. This one has gone batshit crazy on me on black agar, but looks super cool and a bit angry. I’m going to let the rhizo strands grow out a bit more and take T1 transfers from those.




Also, could someone please tell me what is being referred to as SSLC and LI?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: KlarnEvil9]
    #29198289 -

KlarnEvil9 said:
I tried leather punches, but I felt like if I got it hot enough to sterilize, it might burn through the plate and the table before it slowed down. Too much mass. Or maybe it didn't need to be all the way glowing red.

:shrug:


No they don’t need to glow red. They are thicker and heavy and the handles will get hot and retain heat, making them too hot to handle. Ask me how I know this. :rolleyes:
You just need to flame it a bit with a cigar torch lighter. What I’ve been doing is wiping it first with alcohol and then torching it for like 5-10 seconds. Then I dip the tip in a spare agar dish until it stops sizzling.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112]
    #29198315 -

SSLC - Semi Solid Liquid Culture
LI - Liquid innoculant

LC is a nutrient rich broth that's been colonized by myc - good for mass production of spawn and fast colonization. All or nothing though, as far as contamination, so tread carefully.

LI is different bc it's myc suspended in distilled water instead of nutritious broth. Ive never done it, but I think there's more than one way to skin that cat, as far as making it.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman]
    #29198348 -

ThurmanMurman said:
SSLC - Semi Solid Liquid Culture
LI - Liquid innoculant

LC is a nutrient rich broth that's been colonized by myc - good for mass production of spawn and fast colonization. All or nothing though, as far as contamination, so tread carefully.

LI is different bc it's myc suspended in distilled water instead of nutritious broth. Ive never done it, but I think there's more than one way to skin that cat, as far as making it.


I thought the LC was liquid culture. But wasn’t sure what the SS was. LI I wasn’t familiar with, but can’t you store myc covered agar in sterilized distilled water?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 1
    #29198375 -

Yep ya can. Basically just take an agar transfer to sterilized water, and you've got long term storage. Thanks for the reminder - I need to order cryovials!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 2
    #29198463 -

AZ Starman2112 said:
Also, could someone please tell me what is being referred to as SSLC and LI?


Someone answered but I wouldn't mess with it until you master getting perfectly clean cultures from spore to shroom, and they're not needed unless you're doing a commercial grow.

Search "liquid" on this for more info,
https://www.shroomery.org/forums/showflat.php/Number/27646632#27646632


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 1
    #29198479 -

These are amazing! I'll have to find a vendor in Europe, the sizes are amazing at how versatile they are!


--------------------
Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.

Cybesa's Ultimate woodlover project



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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 4
    #29198543 -

nektar61 said:
they're not needed unless you're doing a commercial grow.



why limit yourself?


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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