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Re: Ask Quick Questions, Get Quick Answers [Re: Shroom_Ed65]
    #29179581 -



sorry I am still learning how post on here also, (apparently)
here is a pic of the whole tub

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Re: Ask Quick Questions, Get Quick Answers [Re: Shroom_Ed65]
    #29179623 -

What do you think is the best way to get rhizo?


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Re: Ask Quick Questions, Get Quick Answers [Re: gmj]
    #29179651 -

As far as I know, there's no sure fire way to get it to express in vitro, as it's a genetic trait that's influenced by environment but not caused by it.

So, run more plates from germ.  More chances of seeing it in vitro.

Changing up media might help too :shrug:  But that seems like a pita outside of water agar.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: gmj]
    #29179670 -

Can you elaborate a lil?


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: gmj]
    #29179681 -

gmj said:
What do you think is the best way to get rhizo?



If you take a few grains from your colonized jars before you go to your sub and place them on a plate you will see Rhizo take off.

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29179717 -

Question: my tub is far from being fully colonized (pictured below), but today I just noticed a little pin in one corner. Should I be worried about this, or is it just normal? Is there anything I should do? I really don't want to get a failed flush





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Free will is an illusion
And that's the most freeing thing in the world
Every molecule in existence already knows its path
So don't worry about knowing yours

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Re: Ask Quick Questions, Get Quick Answers [Re: neuralnautwork]
    #29179719 -

I have a few prints that I'm wanting to start using. I saw a post a couple days ago about using a loop by cooling it in agar and using that to pick up a few spores.

I'd rather not use the entire print for just a couple plates. If I do the above method in a SAB, will that make the print dirtier so that any future attempts have a higher chance of failure? Can I just fold the print back up and save it for later?

Just wondering if I should do the swab method instead? That way I can make several swabs and just use one.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29179729 -

PuffM4gicDr4gon said:
I have a few prints that I'm wanting to start using. I saw a post a couple days ago about using a loop by cooling it in agar and using that to pick up a few spores.

I'd rather not use the entire print for just a couple plates. If I do the above method in a SAB, will that make the print dirtier so that any future attempts have a higher chance of failure? Can I just fold the print back up and save it for later?

Just wondering if I should do the swab method instead? That way I can make several swabs and just use one.


You can reuse the print. Sterilize the loop, cool it in the plate you will streak (added bonus of slightly wetting the loop which will help spores stick), scrape a section of the print and the streak the loop on the plate. You should be able to leave most of the print undisturbed and you can fold it up for future use.





Any ideas on why these transfers change from rhyzo to tomentose half way through?

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Re: Ask Quick Questions, Get Quick Answers [Re: space_shrooms]
    #29179740 -

Was it different recipe of agar, from the wedge?:shrug:

@Puff


A normal size cube print can be used to make quite a few germ plates

If you can see the spores on the plate, it's more than enough.

Pan prints on the other hand are so tiny, but still have an abundance of spores


Edited by LewDoja (04/05/25 09:20 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: neuralnautwork] * 2
    #29179747 -

neuralnautwork said:
Question: my tub is far from being fully colonized (pictured below), but today I just noticed a little pin in one corner. Should I be worried about this, or is it just normal? Is there anything I should do? I really don't want to get a failed flush







Next tub pay more attention to the leveling and thicknes of the top layer...

But hey, if a sub has a fruit it's not a failure.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29179754 -

Yeah.. this is my first real grow and I've been finding a LOT of things to do differently the next time around :facepalm:

Do you think I'll get a normal flush out of this? or is the poor thing doomed


--------------------
Free will is an illusion
And that's the most freeing thing in the world
Every molecule in existence already knows its path
So don't worry about knowing yours

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Re: Ask Quick Questions, Get Quick Answers [Re: neuralnautwork]
    #29179761 -

I don't see any obvious reasons why it wouldn't be.


The darker spots of coir are a little concerning, but with such an un even sub it's probably just where the condensation accumulates.


How's the fae?

Looks like an unmodified tub....

Are you flipping the lid?

Edited by LewDoja (04/05/25 09:34 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Shroom_Ed65]
    #29179764 -

Shroom_Ed65 said:


sorry I am still learning how post on here also, (apparently)
here is a pic of the whole tub



Is that colored area a reflection off of something or is it actually on the sub?

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29179778 -

The dark spots are just uncolonized I believe, I didn't do a great job of evenly spreading the spawn. Here's a picture of the same tub on the 30th.



This is the unfortunate thing about my grow... One of my many mistakes was making the substrate too shallow, so it doesn't retain moisture very well. I let em start growing fully sealed, then ppl on here urged me to flip the lids so I did, and they dried out and shriveled in a day and a half. So I took a few days rehydrating the coir, now I'm cracking the lids for a few hours a day and leaving em sealed the rest of the time so they don't dry out again.


--------------------
Free will is an illusion
And that's the most freeing thing in the world
Every molecule in existence already knows its path
So don't worry about knowing yours

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Re: Ask Quick Questions, Get Quick Answers [Re: neuralnautwork]
    #29179796 -

dialing in FAE is most of the nuance of tub growing.  you can side-watering to rehydrate the cake.  with side watering you're just heavily misting the walls or pouring water down the sides, waiting for the cake to absorb it, and repeating until you hit your target hydration point.

generally speaking cubes do not need much FAE.  if you're not giving them enough, the fruit will show obvious signs and let you know (excessively fuzzy feet, elongated/tapered stipes, small caps).

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29179802 -




Found this spot on one of my cakes, removed it from the container - Quick Question are the rest of my cakes fucked?


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Our Collective Being, The Divine Artist - the Creator God, Aching to express, to exist, Exploded itself/ourself into a cascade of light selves. A cosmic orgasm, Big-banged us into creation,Perfect in ratios, rhythms, and forms. The universe is a storm of light emerging. - Alex Grey

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29179807 -

the white is the mycelium growth, the darker spots are where the substrate is still showing a bit, I'm a first time grower also so it can be a real learning curve I'm finding out, but there are some great people on here that's helped me alot.

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Re: Ask Quick Questions, Get Quick Answers [Re: Shroom_Ed65]
    #29179810 -

The pink towards the bottom?
Quote:



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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29179817 -

LewDoja said:
Was it different recipe of agar, from the wedge?:shrug:



Yea. That's probably the reason :thumbup: will likely chill out in a couple more transfers

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29179828 -

LewDoja said:
With a new blade, and you don't cut it on a cutting board.

Holding it, the new blade can easily slice through the cotton. An old blade will just tear it but still effective.


Interesting.  Could you not also pre-make some little cotton puffs, wrap and sterilize them, and then collect spores and inoculate with tweezers so you don't have to do any cutting at that stage?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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