Damnlamb said:

Thanks! The smell was a fairly big tell but nice to have a definitive answer

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CranberrySteve said:

psilygoose said:

That pretty much is what I have been doing lately, at least for spore to agar, my plates do live in my grow room. I don't always use Rye water mostly because I've been trying different things.

But... how do you make agar from substrate?

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Did you notice faster growth on those rye plates?

I was just going to grind up some dry coir in a magic bullet and add it along with grain soak water, and maybe some peptone. I don't really care if it's got bits in it, and I'd hope the mycelium would appreciate some chunks of lignin and celluloses. I'd also try and keep the nutrient level fairly low, so like 0.5% peptone, half diluted grain soak liquid and a pinch or so of coir per 500ml. I dunno, I'm just spit balling

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Damnlamb said:

Wondering if anyone can confirm if this is contamination or not, first time I've experienced these symptoms which are: really fluffy mycelium or mold that has attached to tub walls and a fairly strong vinegar/ferment smell. The why is easy for me to guess, they were accidentally deprived of fae while I was traveling but unsure if should be binned or not

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psilygoose said:

CranberrySteve said:

psilygoose said:

That pretty much is what I have been doing lately, at least for spore to agar, my plates do live in my grow room. I don't always use Rye water mostly because I've been trying different things.

But... how do you make agar from substrate?

---

Did you notice faster growth on those rye plates?

I was just going to grind up some dry coir in a magic bullet and add it along with grain soak water, and maybe some peptone. I don't really care if it's got bits in it, and I'd hope the mycelium would appreciate some chunks of lignin and celluloses. I'd also try and keep the nutrient level fairly low, so like 0.5% peptone, half diluted grain soak liquid and a pinch or so of coir per 500ml. I dunno, I'm just spit balling

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Fastest growth I had so far is this recipe:

500 ml distilled water
10 ml Karo
1 gram peptone
1 gram LME

Note that there's lots of other variables and I haven't done anything scientifically. I was actually thinking about cutting the Karo and putting in some rye water. Anyway, I'm not an expert, that's just what I've seen in the very limited work I've done so far.

I'll leave it to some experts with more experience to say what issues might be caused by putting substrate in your agar. One issue for me would be that I think it might make it harder to spot contam.

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Not the prettiest of plates. I ground up some corn and coir, boiled it, let it sit, poured off 100ml of the liquid, added water up to 500ml, 0.5% peptone. Now we wait I guess.

CranberrySteve said:

Not the prettiest of plates. I ground up some corn and coir, boiled it, let it sit, poured off 100ml of the liquid, added water up to 500ml, 0.5% peptone. Now we wait I guess.

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psilygoose said:

That's a bit rough, I would have run the corn water through a coffee filter.

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Jrey16 said:

I also have some plates that look like this. When you look at it those dark specs are actually white but when you put it into the light it looks dark. Anybody know what those specs are and will I be able to transfer off of plates like these?

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tholos said:

Jrey16 said:

I also have some plates that look like this. When you look at it those dark specs are actually white but when you put it into the light it looks dark. Anybody know what those specs are and will I be able to transfer off of plates like these?

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what does the plate the transfer came from look like? are the two plates from the same agar recipe? the growth looks weird. is that APE redux by dichotomouskeys?

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Jrey16 said:

It came from a swab. I took a transfer from a swab i stuck in the middle of the plate. It actually had nice rhziomorphic growth. And yes the same agar recipe, only difference is the agar that was swabbed had red food coloring. I was told it was APE redux but I didn't know who made it, I didn't get it from dichomouskeys.

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GenesisCorrupted said:

Are people still having a problem with telephone agar?

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