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flapping_bird
Human, Be-ing

Registered: 01/03/25
Posts: 1,812

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Morinsana1]
#29166672 - 03/23/25 09:02 AM (1 year, 5 months ago)

Nice work! I also use a Gordobox and love it! Don't let any of the haters dull yer shine, dude! Laminar flow or not, if it succeeds in keeping your plates free from contams, that's the only thing that matters.

Rock on!

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JW123
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Registered: 01/17/22
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: flapping_bird]

5
#29166678 - 03/23/25 09:16 AM (1 year, 5 months ago)

Explaining how and why laminar flow works is not being a hater. :lol:

:lol:

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,702
Loc: bioilliminicent©️

Re: AGAR ENVY! (Anything and All things agar!)

[Re: JW123]

1
#29166745 - 03/23/25 10:47 AM (1 year, 5 months ago)

JW123 said:

Explaining how and why laminar flow works is not being a hater.

:whathesaid:

That being said, I’m a gordo box hater. It’s not real clean work and it’s a ridiculously expensive getup compared to a seven dollar SAB that is more effective. If you want a flow hood then build a real flow hood :shrug:

:shrug:

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Rooster87
The mountains are calling

Registered: 01/02/25
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: JW123]

1
#29166805 - 03/23/25 12:08 PM (1 year, 5 months ago)

JW123 said:

It's kind of hard to see what is going on with the mycelium. It looks to me that the discoloration is the mycelium eating the food coloring. The weird stuff that happens with colored agar is the main reason I quit using it. I would grow that transfer out and see how it looks.

Yeah I dumped the rest of the unused ones from that batch and have a new batch in the PC now with no food coloring. The myc is spreading very slowly compared to some other transfers and clowns so I’ll see what that one does over the next couple weeks. Thanks for the reply.

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Huskies
Boop More Snoots

Registered: 03/22/16
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rooster87]

7
#29166967 - 03/23/25 03:25 PM (1 year, 5 months ago)

APE Plin plate on Gnocchi Agar with Nutritional Yeast and Maple Syrup

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Krakencaps
Alchemist

Registered: 03/21/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huskies]

1
#29167462 - 03/24/25 06:10 AM (1 year, 5 months ago)

Cleaned up this ms culture

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Churpee Choozle
Grossly contamed

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Krakencaps]
#29167712 - 03/24/25 01:38 PM (1 year, 5 months ago)

I josexed this out of a mexicana 'Rancho Agosto' germ plate that showed mostly green. It is the tiny hole in the biggest colony. Can anyone confirm if this is indeed Rancho Agosto? It sure doesn't look like my galindoi used to do.

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Smellyhobbit
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Registered: 04/01/22
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Churpee Choozle]

11
#29168069 - 03/24/25 07:54 PM (1 year, 5 months ago)

Ovoid germ plate

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oat bag
a.k.a. 2024-12-11

Registered: 12/11/24
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Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]

6
#29168185 - 03/24/25 10:49 PM (1 year, 5 months ago)

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]

1
#29168215 - 03/24/25 11:44 PM (1 year, 5 months ago)

Smellyhobbit said:

Ovoid germ plate

:wobble:

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chfbrd
Stranger

Registered: 09/22/23
Posts: 131
Last seen: 1 year, 9 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#29168342 - 03/25/25 04:35 AM (1 year, 5 months ago)

Hello,
I am trying to cross strains...is this looking like it might work? you can see in the pics, the before and after of where I took samples from. Does this look like the correct place to get a sample from? Does it look like the PP is taking over the TP sample?
thanks

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san pedro guy
Captain

Registered: 10/22/17
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: chfbrd]

3
#29168429 - 03/25/25 07:17 AM (1 year, 5 months ago)

I believe to cross strains you mix the spores together and hope they make your desired mycelium. Not totally sure just intertwingling the mycelium will do anything

check out this strain crossing thread

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JW123
Nooby Nooby Noob

Registered: 01/17/22
Posts: 3,103
Loc: Tn
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: san pedro guy]

1
#29168438 - 03/25/25 07:26 AM (1 year, 5 months ago)

Here is another one where faht used other methods.
https://www.shroomery.org/forums/showflat.php/Number/28117746/page/1

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Huskies
Boop More Snoots

Registered: 03/22/16
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Last seen: 9 hours, 49 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: JW123]

1
#29168483 - 03/25/25 08:14 AM (1 year, 5 months ago)

Starch water from boiling vermicelli noodles. Ingredients: Rice Flour, Water

I think this might be an interesting base for agar. Gnocchi water is my go to, I tried ramen boil water, but those ramen noodles have a lot of palm oil and sodium and it would leave swirls on top that could be confused for bacteria.

The Vietnamese vermicelli was way less ingredients than the gnocchi, so I wonder if it will create less sediment.

On that note, last time I did my gnocchi water agar, I tried straining it because there were a lot of starch solids that never mixed perfectly. It's still working but growth is less impressive. Last time I also added nutritional yeast and things exploded pretty quickly on those plates.

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tholos
Stranger

Registered: 03/06/23
Posts: 666

Last seen: 1 year, 24 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: chfbrd]
#29168536 - 03/25/25 09:18 AM (1 year, 5 months ago)

chfbrd said:

Hello,
I am trying to cross strains...is this looking like it might work? you can see in the pics, the before and after of where I took samples from. Does this look like the correct place to get a sample from? Does it look like the PP is taking over the TP sample?
thanks

These are both dikaryotic, you need the monokaryotic forms of each, then you can use this method to cross the strains

edit: from one of the links provided above

cronicr said:

Fruits are made of dikaryotic mycelium
Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit.
When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon.

Edited by tholos (03/25/25 09:21 AM)

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Carnonos
The Horned Wanderer

Registered: 02/05/23
Posts: 572
Loc: Terra
Last seen: 1 year, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tholos]

4
#29168540 - 03/25/25 09:22 AM (1 year, 5 months ago)

ochra clone I'm currently playing with - lookin pretty for a T2

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AZ Starman2112
Male Half of a Friendly Couple

Registered: 12/11/24
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Carnonos]
#29168566 - 03/25/25 09:46 AM (1 year, 5 months ago)

Just out of curiosity can a piece of grain covered in mycelium be put on agar to obtain a clean culture? Say if the grain was taken from a jar that was contaminated in one spot, but clean in other places.

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oat bag
a.k.a. 2024-12-11

Registered: 12/11/24
Posts: 183
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

3
#29168574 - 03/25/25 09:51 AM (1 year, 5 months ago)

Yeah. You might bring over some contamination that isn’t yet visible but you can clean it up with transfers :awesomenod:

:awesomenod:

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,702
Loc: bioilliminicent©️

Re: AGAR ENVY! (Anything and All things agar!)

[Re: oat bag]

2
#29168577 - 03/25/25 09:54 AM (1 year, 5 months ago)

If you want to be real thorough you could drop a grain on a BRF puck and transfer that growth to a plate.

--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,960
Loc: South Africa
Last seen: 48 minutes, 59 seconds

Re: AGAR ENVY! (Anything and All things agar!)

5
#29168579 - 03/25/25 09:56 AM (1 year, 5 months ago)

Maketi grain on its way to agar.

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