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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29150591 -

tree frog said:
I usually dilute it by half for plates.  That said, it's really not recommended because grains can be sprayed with fungicides that will run off in the water.


You mean that grain culture in general is not recommended or diluting it is not recommended?


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FYI: I am having uploading pics to Shroomery (says the file is too big, even with small screenshots) so any pics I post are linked to another site

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Re: Ask Quick Questions, Get Quick Answers [Re: Big Jon] * 3
    #29150603 -

The concern is that the possibility of fungicides present on the outside of the grain makes the boil/soak water a poor choice for a nutritive media like agar or liquid culture.

The only problem I've personally had with grain water is it's hard to make media with a consistent makeup, so different batches result in different growth patterns, and that can make it hard to narrow down causes of slow or wispy (or otherwise suspicious) growth.

I kinda like it more for LC than for agar, because I'm putting pretty confidently clean culture into LC, and small recipe differences don't seem (to me) to make as big a difference with how the mycelium grows in liquid culture.

If you rinse the grain first, I think the boil or soak water should be just as reasonable to use for culture as the hydrated grain. Like if it's really got a bunch of fungicide that can't come off with a rinse, it's going to soak right onto the grain when hydrating it, which would also make it a shitty choice for spawn.

When I use it, I also usually dilute, 1:1 at the strongest, often 1:2 or 1:3.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Big Jon]
    #29150617 -

Big Jon said:
Recently just purchased some Milo grain for truffle making, and decided to make grain culture. I just used hamloaf’s super simply milo prep, and when I strained the water, collected it in a mason jar, sterilized 15 PSI for 20 min but it came out dark. I can barely see the mycelium moving around (but my LME LC - .25 g in 150 ml distilled H2O - that I made with the same syringe, is looking great!).
All the pics I see of grain culture on here are lighter looking, but I can’t find a tek that explains exactly how much water to add.

So my question is, how much water should I add to grain water or am I good and I don’t NEED to make it lighter, it’s just a preference??


Stick with LME.
Unless you're in a pinch. 

But honestly I don't recommend LC either way, but everybody will do as they please (as they should).


SSLC, if you insist...

But to answer, cut it at least 50% after settling with decant.

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Re: Ask Quick Questions, Get Quick Answers [Re: Big Jon]
    #29150618 -

Big Jon said:
tree frog said:
I usually dilute it by half for plates.  That said, it's really not recommended because grains can be sprayed with fungicides that will run off in the water.


You mean that grain culture in general is not recommended or diluting it is not recommended?



They are referring to grain water being used for more than just hydration of said grain.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29150674 -

Do spores from bigger shrooms tend to produce bigger shrooms, or is that not a thing?

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine]
    #29150704 -

maplepine said:
Do spores from bigger shrooms tend to produce bigger shrooms, or is that not a thing?


There is the possibility of those genetics being passed down yes. But, just like people, just because you have two tall parents doesn't mean you will also be tall.

But if you see a mushroom you like, you can always clone it, run that clone to fruit, then clone the next one that looks similar to what you want. Cloning gives you a much higher chance of seeing similar fruits to to your original clone in your tubs.

Keep doing that and eventually over a bit of time you will have a tub full of exactly what you want.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine] * 1
    #29150707 -

maplepine said:
Do spores from bigger shrooms tend to produce bigger shrooms, or is that not a thing?


Can but not always. As said above, "There is the possibility of those genetics being passed down yes. But, like people, just because you have two tall parents doesn't mean you will also be tall."

Every time spores go to agar, it's a crap shoot, and thousands or millions of strains are produced. Overall, some or most have some relevance to the parent shroom but not always.

To create a variety (true name for what's commonly and wrongly called a "strain"), you have to select for a trait and go 8 or more generations of spores, grow, pic, best shroom, take spores, etc.

If you want your shrooms to be very close to their parent shroom, cloning is needed. Can still some variety though, and if you take spores from a clone and you grow out the spores, you're back of the lottery.

It's part of the fun of shrooms.

I spent way too much time worrying about this at first, as do most people. If I had it to do over I'd have spent all my time at first learning how to grow clean, contam-free shrooms, and get good at doing agar transfers, and growing lots of shrooms. 


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (03/09/25 04:39 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29150735 -

Would you guys send these 2? The glass is opaque 'cause of the condensation they had.



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Re: Ask Quick Questions, Get Quick Answers [Re: Nerex] * 1
    #29150788 -

You guys helped me ID these but when should I harvest them, pic is of today


--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29150794 -

This is probably a dumb question since I already did it, but will reishi grow on a BRF cake? I spooted some agar plate LI juice into a qt PP5... maybe this should be a confession?


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29150802 -

So I just did an agar transfer. This is my first time doing and wanted to get all your thoughts onto weather it looks fine at the initial stage. I did the transfer 4 days ago. Does it look OK?

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29150825 -

KlarnEvil9 said:
This is probably a dumb question since I already did it, but will reishi grow on a BRF cake? I spooted some agar plate LI juice into a qt PP5... maybe this should be a confession?


Likely, yes.  I'm not sure if it will fruit without wood.  But I'm almost certain it will colonize it.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29150872 -

tree frog said:
KlarnEvil9 said:
This is probably a dumb question since I already did it, but will reishi grow on a BRF cake? I spooted some agar plate LI juice into a qt PP5... maybe this should be a confession?


Likely, yes.  I'm not sure if it will fruit without wood.  But I'm almost certain it will colonize it.


Awesome, thank you for the knowledge. I have some HWFP in the garage for fruiting, but I finally got motivated yesterday and maybe got carried away in the SAB... :shocked:


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29150877 -

Never harvested PE before and wanted some input. OK they can go again, because for as big and hard and phallic as they get, they don't really "bust seed" as well as many other strains, and so many like to wait until they peirce the veil(veil breaks and/or Mychael Stipe gets his lumpy head back into an REM show. . .what do you guys think . . .RIGHT tf bow, later today, or another day or two? I Have legit severe OCD and am thus a very impatient rabbit, due to my symptoms, and I've been wanting to since pin(and since before colonization, or even having the bag) and am getting envious of all the phallus.  Michael Stipe is a bit lumpy on my big boi and a few others. . .  Am I missing out on much dry weight by doing it now?  Missing out on anything else good, if doing it later?

Also another question.  To dunk, or not tondunk?  Are you feeling lucky, PUNK!?🔫🤠




Sorry for being so "pun-k rock" in this post. . .and in general. Apperantly I'm rly on one today.  Haha.

Edited by sk8cracka (03/08/25 11:27 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #29150894 -

towerofbabel said:
So I just did an agar transfer. This is my first time doing and wanted to get all your thoughts onto weather it looks fine at the initial stage. I did the transfer 4 days ago. Does it look OK?



Looks great, nice job!
You can get that sediment into solution if you cook it in a pot first with lots of stirring, adding ingredients to cold water first.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
    #29150905 -

Another (few?) Question(s?)  I JUST took my first foray into making agarband don't really 100% know what to look for.  The 3 on the left are from that GT grain that a homie or 2 l on this thread said looked like it may be bac, and the 2 on the right are from the Bluie V LC that somehow got an ant or 2 in it[ FML], either through the lid screw-in shitte thing, or trough my micropore, and I put on the agar a day or 2 prior to the GT. To test and try to isolate.  How's everything looking?  I do see a bit of gnarliness that may need to be tossed, but not in the dime yet.  (some of the GT have a few spots where grain was dropped, which there are multiple colonies)

And i made this one the same day I mixed the blue agar, and dropped LC on a few, but I don't think I put LC on it unless it ended up mixed in with the non-innoc'd ones.  Does it look like contam? Or like myndumb, absent-minded arse may have inno'd it and spaced it. I assume myc tends to be lumiere or webbier, but ik there are a few type
Grains were 5th or 6th, and lc was 4th if that's of any importance.

And yes. Some of those gnarled chunks in the agar ARE INDEED chunks of dry hashbrowns my dumb ass didn't think to grind. They were mainly in the later pours from that batch. And they are quite condensation-ie but I do tend to store upside down to be safe. (Which I only ASSUME still applies to condiment cups)

Big apologies on the noobary.  I have only worked with fungi two times before this, and all 3 have been about a decade apart...trying many(and since 2015 there ARE Many in general)new things(and wanna at least go until the cold season starts back up, this time)

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Re: Ask Quick Questions, Get Quick Answers [Re: sk8cracka] * 1
    #29151068 -

Hello. All but that last pic are too blurry to see anything. That last pic looks like bacterial biofilm (to quote our friend Stubb). Any LC with an ant in it is garbage.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
    #29151112 -

LadysKnight said:
Hello. All but that last pic are too blurry to see anything. That last pic looks like bacterial biofilm (to quote our friend Stubb). Any LC with an ant in it is garbage.


For sure thanks. The ant agar cultures was literally just to experiment and check what the culture turned out looking like. . .but ants def aren't sterile, and if there is enough air exchange for 2 ants, doubly so.
Surprisingly the jars I did the day I noticed the ants(before hand and way before the agar) are doing great thus far but I'm expecting that to change
.

And if I can somehow get a big enough clean spot on an "anty agar culture" and isolate until we gucci? Then even better. There's enough vegan seaweed jello to go 'round. :smile:  oh and the "blur" is mostly condensation I think.  For future ref.  To stab a hole or 2 in the upper sides of cup part or not to?

Any input on the PE harvest time based on that photo?  They aren't Hella soft yet, but the stipe are getting lumpy, and obvz veils hath broken.
Idk if you cultivate pe or have at all but if someone has and could give some input to an impatient bunny it'd be appreciated?

Anyone wanna put in a fun poll for approx dry yield for the first flush too? Lol

Edited by sk8cracka (03/08/25 02:01 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 4
    #29151136 -

Kloakk said:
fahtster said:
I’m with having an extra gram on deck just in case.  The redose isn’t (usually) as intense of an experience.  You’re not going through a come up like the first dose.  It just intensifies what you’re already experiencing and a gram of cubes is pretty light for a redose.  No matter what happens, just keep in the back of your mind that it’ll pass.  More than likely, you’ll have a great time.  If things get a little intense, throw on your favorite music or pop in a comedy and laugh your ass off and just take the ride because it will pass.


Man, I can feel your words. That introduction to shrooms.. It's pretty sick how shrooms are so safe, but can feel like the scariest thing ever...I mean, I've had two life changing experiences on +6g semilanceata...But this is sound advice, "it'll pass".



Definitely try to stop telling your self your scared.    Be prepared and ready to take what it gives and go along for the ride.  And when you are tripping make sure you try hard not to tell yourself "man this is too strong" or "man I took too much" or a
"man I made a mistake I wish this wasn't happening" . Cuz that'll absolutely make your trip paranoid and scary. You've got to tell yourself it's only a matter of time I know I ate a substance a d thats why im tripping. and before long I won't be tripping anymore.


As a beginner I always said 2 G to start off if you've never taken it or the variety before. And then you can always add another if you need to. That will ensure you get some effects and not bummed out by the come up and then nothing happening. Nowadays I start with 3.5 to 4 and that'll usually ensure I don't have to take anymore and have a blast.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 1
    #29151279 -

Awesome! Thanks for the tip!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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