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Re: Ask Quick Questions, Get Quick Answers [Re: lushmush]
    #29148136 -

lushmush said:
Hey all, this is my second grow and my first time dealing with contam. My first grow did amazing but this time I have a dime size spot of contam. How would you go about dealing with this? Is there a way to take care of it or is it is what it is?




Separate it from the rest of your tubs (different room/ garage) and let it ride, it'll most likely trich out, but if you leave it with the rest of your grows you can chance losing all of them.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29148158 -

Nah, that's not really the case.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29148224 -

Interesting, for some reason I must've remembered reading that somewhere, so it doesn't spread like that once it sporulates?

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 4
    #29148248 -

Axman said:
Interesting, for some reason I must've remembered reading that somewhere, so it doesn't spread like that once it sporulates?



That was certainly previous consensus, at some point. I mean, it seems logical. But the truth is, we are constantly swimming through a soup of contam known as air. It's just everywhere all the time anyway.

2nd part is, if your spawn is clean and fully colonized, you can fruit it in the toilet and it will not contaminate until the mycelium gets weak enough to allow it.

Your efforts are appreciated Mr Axman, thank you for helping out around here.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
    #29148274 -

LadysKnight said:
Axman said:
Interesting, for some reason I must've remembered reading that somewhere, so it doesn't spread like that once it sporulates?



That was certainly previous consensus, at some point. I mean, it seems logical. But the truth is, we are constantly swimming through a soup of contam known as air. It's just everywhere all the time anyway.

2nd part is, if your spawn is clean and fully colonized, you can fruit it in the toilet and it will not contaminate until the mycelium gets weak enough to allow it.

Your efforts are appreciated Mr Axman, thank you for helping out around here.



What do we know about mycelium gets weak enough to allow it? I know that with my Ochras, even though I was getting full canopies and 1000g wet, they would almost always mold immediately after the first flush. Perhaps it was leaving stems.

Should start again from spore, been focusing on other things.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (03/06/25 10:29 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 5
    #29148276 -

Your environment, more or less:



Mycelium protects nutrient rich material by colonizing it, essentially covering grains with an immune system. Contaminated Mycelium has a compromised immune system which allows any nutrient dense materials to be exposed to illness, which will present itself as mold or bacterial infections.

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #29148280 -

I started using an air purifier around the house, been doing that more and it does seem to have helped. Grow room is quite clean according to it, but I do make the tubs elsewhere. Maybe that's been a dumb vector.

Although I think my problem has been uncolonized millet grain stuck on the lid. I started wiping down the top sides and lid of jars at spawn before breaking them up and that does seem to have helped.

Went back to oats and I think I prefer it now, bigger but more robust growth, less kernels, more mass and water in each quart.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap] * 4
    #29148285 -

If you think about it, how the idea of contamination spreading came about kind of makes sense. We do large batches of Spawn at a time, but every tub doesn't contaminate at the exact same rate. It's entirely possible for one tub to show contamination and then another one to show it a couple days later. This would give the impression that the contamination spread to a healthy tub when in reality the other tub was contaminated already.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29148286 -

Huskies said:
LadysKnight said:
Axman said:
Interesting, for some reason I must've remembered reading that somewhere, so it doesn't spread like that once it sporulates?



That was certainly previous consensus, at some point. I mean, it seems logical. But the truth is, we are constantly swimming through a soup of contam known as air. It's just everywhere all the time anyway.

2nd part is, if your spawn is clean and fully colonized, you can fruit it in the toilet and it will not contaminate until the mycelium gets weak enough to allow it.

Your efforts are appreciated Mr Axman, thank you for helping out around here.



What do we know about mycelium gets weak enough to allow it? I know that with my Ochras, even though I was getting full canopies and 1000g wet, they would almost always mold immediately after the first flush. Perhaps it was leaving stems.

Should start again from spore, been focusing on other things.



I tried reviving a 3 year old dried out plate that didn’t have signs of contam and all that grew was mold, some trich and some other things i assume.

Seems like mold will always be present but full colonization allows the preferred culture to grow.

does that mean our preferred cultures are actually the weakest?

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap] * 1
    #29148293 -

Thank you all for clearing that up.

@ladysnight
Thank you, and that got me thinking.

I'm pretty sure, that with clean spawn, you can fruit anywhere.

I had to look to find it but your toilet analogy reminded me of Pasty's Bathtub Grow.

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 6
    #29148301 -

Positive pressure in your "lab" space certainly helps keep airborne particles out of your sterile media when working.

People don't think of cross contamination.  If you have spawn on deck, enough for multiple tubs, each tub is mixed by hand, usually without gloves. Then you load up the next tub and do the same, repeat until finished. Your first 2 tubs may have been spawned with clean spawn, but the 3rd had a single jar with a slight bacterial infection that went unnoticed. Tubs 4 and 5 are now contamination by tub 3, via cross contamination.

Each tub should be spawned individually as though you're using raw chicken. Do not break up spawn using the same gloves for each tub, otherwise you're potentially spreading contamination between tubs.

Sure, you can spawn in open air without problems, but this doesn't apply to cross contamination. 

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap] * 2
    #29148312 -

Stipe-n Cap said:
Positive pressure in your "lab" space certainly helps keep airborne particles out of your sterile media when working.

People don't think of cross contamination.  If you have spawn on deck, enough for multiple tubs, each tub is mixed by hand, usually without gloves. Then you load up the next tub and do the same, repeat until finished. Your first 2 tubs may have been spawned with clean spawn, but the 3rd had a single jar with a slight bacterial infection that went unnoticed. Tubs 4 and 5 are now contamination by tub 3, via cross contamination.

Each tub should be spawned individually as though you're using raw chicken. Do not break up spawn using the same gloves for each tub, otherwise you're potentially spreading contamination between tubs.

Sure, you can spawn in open air without problems, but this doesn't apply to cross contamination.



I spawn with this in mind. On spawn day, I rate my spawn and organize them from best to least and spawn in that order. My observations are usually right on with the results. But the point is not to carry funk forward.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 3
    #29148316 -

This could account for any gap between tubs showing contamination. The tub with the contaminated spawn will show first due to higher contaminant load, the cross contaminated tubs will lag behind but will eventually catch up, due to a lower contaminant load requiring extra time to bloom.

Definitely weed out the weak links. Folks tend to get into a fit when you suggest they toss even slightly contaminated spawn, but fail to grasp the bigger picture. Yeah, you might get some measly fruits, but you risk everything else downstream without realizing it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29148407 -

Huskies said:
Although I think my problem has been uncolonized millet grain stuck on the lid. I started wiping down the top sides and lid of jars at spawn before breaking them up and that does seem to have helped.




Tap your finger on the filter and lid after you shake your jars during colonization. Doing this will dislodge any grains sruck in the GE hole or get stuck to the lid. Shaking your jars will cause the grains to scrape any uncolonized grain off the interior of the lid, any grains that do stick should be colonized.

Chasing variables without confirmation is a great way to keep chasing your tail.

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29148470 -

Huskies said:
Tbh when it contams before fruits even come out, it's likely just going to be a total fail. It happens, don't get discouraged. Try cutting out basically half of the sub, 2/5 of it, and make the cut line far enough away from the contamination.

Then pray for meager success, but it is likely a tosser. If you see it spreading elsewhere after cutting it out, don't even hesitate and just toss it.



Or bury it if it's not freezing outside

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29149041 -

4th day in tub from AIO grow bag with B+. Going to give it a couple of more days


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #29149048 -

4th day in tub from AIO grow bag with B+. Going to give it a couple of more days


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: ultrasuede]
    #29149139 -

Does this subtropicalis plate look like it has bacteria in it?

Front:



Front/backlight:



Back/backlight:





You can see a kinda brownish discoloration around the perimeter of the white in the center.  Looks like spilled liquid puddling.

Ive been told semps can tend to turn a brownish caramel color.  Is that what's happening here? Or could it be shadows from the density of mycelium changing from thicker to thin?


My other plate dosnt have any discoloration..




--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29149246 -

So I have some yetis that are presenting very different than any other yetis I've grown. They're beautiful.

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Re: Ask Quick Questions, Get Quick Answers [Re: crispydatrapboy704]
    #29149252 -

This is it.

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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale


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