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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Jaegur] * 2
    #29142315 -

Jaegur said:
Hello, peeps! Months long lurker, first time poster!

First off- this is my first attempt, so definitely some lessons learned on fanning an inflatable monotub (I did not), but I'm guessing the c02 levels were too high for these guys, and I was just away for 2 days so I couldn't monitor them more closely.

I feel like I will start needing to harvest some of these soon, but the grow seems kind of all over the place, as far as size and whatnot.

Any just general opinions and advice judging on these images? I have a few other tubs in various stages of growth, though none this far along so I'm hoping to course correct early on.





Spots on the caps, stunted growth, and peeling stipes make me think verticillium infection.

Welcome, and good job getting mushrooms on your first try. Your surface conditions look pretty good, maybe could use a bit more fresh air, but not a ton. Work on getting some clean spawn and you won’t have to worry about monitoring. I typically leave my tubs alone until I’m ready to harvest. Fanning and misting should not be a regular part of your process for cubes.

I’m not a fan of inflatable tubs for a bunch of reasons, but can you adjust FAE on those?


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29142407 -

Are these looking good? Oats inoculated with sporex lq early January but I lost heat for a week and it definitely slowed growth, I don’t see any odd colors but didn’t know if the strands were contam?



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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29142528 -

Thank you for the reply! I haven't been able to find much on that type of infection, is it something that would be considered a tub loss and start over?

And unfortunately, I think the only way to adjust fae in the inflatable tubs are to lessen it by plugging up the holes.


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Ah, crap.

Edited by Jaegur (03/01/25 11:49 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jaegur]
    #29142539 -

How much clearance needed from wall to back of 2x2 flowhood?

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Re: Ask Quick Questions, Get Quick Answers [Re: eugenemyco] * 1
    #29142571 -

Several inches if possible.  Can probably get away with 3 but more is better.  I'd say to listen to the fan as you move it closer and when you hear a sound difference pull it away just a bit.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #29142642 -

Hey all!
I'm working on my first batch of cultivating through agar plates, and I wanted to post a few pictures to get some feedback. They've been growing for 9 days.



I've had a hard time finding exactly what to do with the agar at this point. Everything I've read talks about agar to agar transfers, but that seems to always be if there is bad mycelium, and these don't seem to have it. Can I transfer these right to a jar of grain mix? And if so, when do I know it's time for that. Sorry for the questions, but thanks for the answers!

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #29142644 -

Take a transfer from what you think is the healthiest looking leading edge.
You want to see uniform growth. So there’s definitely something going on in that plate.


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29142665 -

:whathesaid:

Those are too splotchy for my likes.

At least one xfer will do You good, take from leading edges (tiny sample)

The first pic 11-2:00 ish  looks like it's starting to organize

Edited by LewDoja (03/01/25 01:36 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Studebaka] * 1
    #29142668 -

Studebaka said:
Are these looking good? Oats inoculated with sporex lq early January but I lost heat for a week and it definitely slowed growth, I don’t see any odd colors but didn’t know if the strands were contam?






Give em a good shake and report back in a day or two

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29142825 -

has anyone used burpee coir bricks? package says "100% coco coir" but they're for gardening so i'm wondering if they're treated with trich. searched and found a couple old threads but nothing definitive. link


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Re: Ask Quick Questions, Get Quick Answers [Re: CultiV8] * 1
    #29142829 -

ya it works :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: Ask Quick Questions, Get Quick Answers [Re: CultiV8] * 1
    #29142848 -

CultiV8 said:
has anyone used burpee coir bricks? package says "100% coco coir" but they're for gardening so i'm wondering if they're treated with trich. searched and found a couple old threads but nothing definitive. link




I believe that being compressed it's clean (of intentionally added trich)

Those are usually bags of loose material iirc

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29142864 -

tripdawg420 said:
ya it works :shrug:


LewDoja said:
I believe that being compressed it's clean (of intentionally added trich)

Those are usually bags of loose material iirc


much appreciated

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29143351 -

Someone just sent me a few "monos" so I can use to cross breed with them. He said i can cross a mono with any other culture of same species or do a Mon/Mon cross. What does he mean by that? Idk what a mon is and I know nothing about cross breeding


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29143427 -

You have very generous hard working friends :biggrin:
“names already suggest, monokaryotic hyphae have only one cell nucleus and dikaryons have two cell nuclei (“mono” meaning “one”, and “karyon” meaning “nut”, “kernel” or, in this sense, “core” or “nucleus”). Dikaryons originate from two monokaryons that fused together and exchanged cell nuclei.“
You can fuse them in a agar plate and sample from the intersection (now dikaryon, the only that will fruit) to grain or you can also, separately, inoculate grain jars/bags and fuse the 2 (mon/mon or di/mon) in a tub
Have fun :rockon:


--------------------
“All this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaedra]
    #29143437 -

Phaedra said:
You’re going to have to experiment here with the consistency and moisture content. If your composted manure is too wet you may have to use a lot of rice hulls. That can increase structure and drainage of a substrate, but they don’t have a high water holding capacity.

I would say try out 25% rice hulls to 75% composted manure and pasteurize.

This doesn’t come from nowhere, I use rice hulls in my garden soil. Lots of good silica and carbon as they break down into organic matter in the soil.


Sounds good, thanks. I could bake in a closed container for either pasteurization or sterilization, or PC for sterilization. I don't want to pour hot water over it! I'm thinking PC will be easier, but is sterilization necessary? Would there be a benefit to pasteurization?

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine]
    #29143454 -

Do grains really need to be not too wet for spawn?

I opened a bag this morning of what I thought was rye, it appeared to be barley. I forgot that. I really like the husk. Anyways, I cooked it and it is now drying, but why actually doesn't mycelium like wet grains, as it loves water with a bit of sugar?

Just trying to get this understood in my head. :smile:

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 3
    #29143468 -

The two biggest issues with wet grains in my experience are clumping and standing water.

If these aren't happening, it's not to wet imo.



These were on the verge of clumping. And likely would have if I wasn't running mixed grains.

They still looked very wet out of the PC, but as they cool the grains absorbed the remaining water.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 5
    #29143475 -

Spawn has to be 100% clean if there's excess moisture.

Any bacteria will have much more motility and proliferate much more easily.

Edited by LewDoja (03/02/25 07:31 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 1
    #29143483 -

cybork said:
Do grains really need to be not too wet for spawn?

I opened a bag this morning of what I thought was rye, it appeared to be barley. I forgot that. I really like the husk. Anyways, I cooked it and it is now drying, but why actually doesn't mycelium like wet grains, as it loves water with a bit of sugar?

Just trying to get this understood in my head. :smile:


Being a bit wet won't exactly hurt the mycelium but it does add benefit for bacteria. Bacteria loves to spread though water. So if your sample is clean wet grain is no major problem but if there is any bacteria at all it will likely out complete the mycelium over time.


--------------------
This hobby has taught me many things.
Most of those things are patience.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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