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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29137505 -

ThurmanMurman said:
ReverendMyc said:
Never thought of that but I like it. Do you prep them like a no pour? Do you shake the jars after a few days and a couple of weeks? Seems like a pretty good way to confirm lids.

I'm gonna do this with the next jar that fails on me. Nice one, sir.



Yes, I treated them like no pours and then let them sit. I didnt shake, but that probably would have been a good idea!




Actually I'd think not shaking would be a better way to test lids/filters, as the location of contamination would be localized to the top, if you've got a spawn prep issue it will be everywhere.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29137508 -

cybork said:
LewDoja said:
cybork said:
The benefit of sourcing it yourself, is that you can better change the levels. Some use 4% sugar (and some 1%), and some higher and some even 0% sometimes. And also for agar you can differ, based on how hard or soft you want the agar plate. I use 4% by default with my standard local brand.

Good luck with your agar journey!




Holy hell batman - 4% agar.

I bet you never have any problems with bacteria, eh?



Yes, 4%. If I do less with this brand, some of the dishes stay liquid. Tried a lot, but all the time with less than 4%. I THOUGHT THAT WAS NORMAL :spoiled:

Not sure what you mean with no problems with bacteria. Frankly most times when I have contaminants, they more look like black and green molds. But not really sure whether those are bacteria or molds. Mostly the things are fluffy, not like wet dirty spots.

Edit+: Don't take my word for the 4% agar. Recipes mention lower %. For example here: https://www.shroomery.org/forums/showflat.php/Number/28959224



Standard is 2% of both agar and nutes, but usually anything higher than  3% (of decent agar) will be too much for most bacteria (at least won't spread very easily).

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29137523 -

Doing transfers today. I'll just try it on a plate and see what happens. It'd be very cool if it actually works


--------------------


Edited by Jrey16 (02/25/25 01:41 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29137560 -

There is this guy that is pretty well known here in my country for growing pans. Looking at his pics and videos it looks like he is growing it outside in open air on the ground.  It looks like he just puts the substrate on the ground outside and mixes the grain spawn in. Is it possible to cultivate pans like that? If so, I'll go ahead and try cultivating pans outside because I'm kind of scared to grow pans for my first time in the same small room that might cause contaminants where I got a bunch of projects and cubes growing. Or maybe I'm just being a pussy lol. But I am running out of space though. We live in a hot and humid country where pans is easily found growing on the ground.


--------------------


Edited by Jrey16 (02/25/25 12:27 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29137670 -

are the lids for Wide Mouth Jars universal?


Like do the wide mouth half pint, Pint, And qt jars all have the same size lids?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29137673 -

No, wide mouth is different to normal qt jars and then you get catering jars that have less thread.

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29137680 -

callmehippie said:
are the lids for Wide Mouth Jars universal?


Like do the wide mouth half pint, Pint, And qt jars all have the same size lids?



Wide mouth quart, pint, and half pint mason jars (at least in the US) have the same exact threads and accept the same lids. Same goes for their regular mouth counterparts.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29137686 -

Groovy. Thanks.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29137688 -

I've got some mycelium on BRF pucks, growing very well. Planning to transfer to agar when there are mushrooms to take biopsies from.

Once the BRF pucks are fully colonized and the mycelia consolidated, should I keep the lids of the jars on, or should I remove them prior to pins/fruiting?


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: Ask Quick Questions, Get Quick Answers [Re: Sarnath] * 1
    #29137692 -

If you leave the lid on, you can take tissue samples from the substrate itself, and clone whole mushrooms (even the outside should be sterile if it's never contacted unfiltered air). They won't grow very pretty without fresh air, though.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29137695 -

Phaethon said:
If you leave the lid on, you can take tissue samples from the substrate itself, and clone whole mushrooms (even the outside should be sterile if it's never contacted unfiltered air). They won't grow very pretty without fresh air, though.



"From the substrate itself", as in taking a small sample of the mycelium surface with an inoculation loop and putting that onto some agar?


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29137718 -

Thurman, that's a fantastic idea! I used a few jars with agar when I had my first SAB. For dry practice. Needless to say that batch grew some nice bacteria. And Lew, he meant with agar, so there wouldn't be any spawn prep issue.

MushMonkey: I only use repurposed 500 - 700 ml jars. I have a 10 qt PC and a very low carbon footprint and intend to keep it that way. Most of my jars have been in use for over a decade. I use repurposed polyfill as filter, change it every five cycles, give or take. If the coating on the inside of the lid starts tearing, it loses it's seal and I change it for a 'new' one. Drill a 8 mm hole, roll a ball of poly and jam it in there. If it's low nutrient substrate I just cover it with some dumpsterdived tinfoil. Like so

(Eryngii on woodchips supplemented with bran, ostreatus on millet)


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29137721 -

Jrey16 said:
There is this guy that is pretty well known here in my country for growing pans. Looking at his pics and videos it looks like he is growing it outside in open air on the ground.  It looks like he just puts the substrate on the ground outside and mixes the grain spawn in. Is it possible to cultivate pans like that? If so, I'll go ahead and try cultivating pans outside because I'm kind of scared to grow pans for my first time in the same small room that might cause contaminants where I got a bunch of projects and cubes growing. Or maybe I'm just being a pussy lol. But I am running out of space though. We live in a hot and humid country where pans is easily found growing on the ground.




It’s how I started growing pans full stop, find an area that doesn’t get more than an hr or 2 direct light a day, preferably under/inbetween bushes or in a garden bed with filtered light, you can spread the grain directly on the surface or mix it in a little and then spread about an inch or 2 of hpoo/cow poo (I mix about 50/50 straw) over the top and lightly water morning and night depending on how much it’s drying out till they pin, do not water the fruits after pinning as you’ll cause aborts but rain seems to be fine if not ideal, I set the hose dribbling and place it next to the areas fruiting for a few mins at a time to maintain hydration 👍

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Re: Ask Quick Questions, Get Quick Answers [Re: BonsaidMycelium]
    #29137762 -

BonsaidMycelium said:
Hey all,

Finally had a small but successful harvest a few days ago. Would like to thank you all for the help.

A little over a week ago I inoculated 6 grains jars with various strains, one in brown rice, one in millet. Followed standard recommended prep methods. Starry Night, JMF, GT respectively. 2 days ago I did break and shake. The starry night recovered really fast.

How am I looking? Yeah, I definitely overfilled the millet jars :crazy:





I've never had good luck with brown rice, it always clumped up on me, but yours looks good.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29137817 -



Am trying to squeeze out a 3rd flush from this tub of GTs, but I’m afraid contamination may have finally caught up. Somewhat new mildewy scent. See upper left quadrant for the small fruits but also some brownish stuff that looks unfamiliar to me. Should I see how this tub plays out or toss it? I was surprised to even see pins at all and there’s a few

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Re: Ask Quick Questions, Get Quick Answers [Re: goodfriend]
    #29137825 -

goodfriend said:


Am trying to squeeze out a 3rd flush from this tub of GTs, but I’m afraid contamination may have finally caught up. Somewhat new mildewy scent. See upper left quadrant for the small fruits but also some brownish stuff that looks unfamiliar to me. Should I see how this tub plays out or toss it? I was surprised to even see pins at all and there’s a few



If it were me, I'd toss it. A moldy/mildewy scent is one of those smells where you should always trust your nose, IMO.


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29137863 -

Hey all!



This is my first time using agar. I wanted to post a picture of the plates and see how they are doing. I put them together on 2/20 so they've had five days for the mycelium to grow. How do they look to you experienced folk?

Edited by towerofbabel (02/25/25 10:26 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #29137899 -

Been reading a lot of the beginner teks, following along with Bods unmodified tub. In that one he just dumps his jars of spawn straight into the tub and mixes it with the coir. Is this the best common practice or is there usually a layer of coir or perlite or something on the bottom of a grow tub.

Seems like his grew pretty well without any kind of holes in the tub and just using the upside down lid to “vent”. Is this typically enough air without using holes?

Have not bought my spawn material yet so if anyone has any suggestions for B+ I’m listening.

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel] * 1
    #29137927 -

towerofbabel said:
Hey all!



This is my first time using agar. I wanted to post a picture of the plates and see how they are doing. I put them together on 2/20 so they've had five days for the mycelium to grow. How do they look to you experienced folk?



They look like they were multispore germ plates and look good, but you don’t need to give contaminants the upper hand by being on that heat mat. 70-75 is just fine for plates, spawn, fruiting, etc. if your room with these is like 50 degrees, maybe look into a dub tub with an aquarium heater, but overall, even mid 60’s is fine with a few exceptions of some exotics.


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29138050 -

Thanks man. What is most shocking to me is how quickly the Starry Night colonized the BR all the way on the left. I did them all on 2/15. It will be fully colonized by tomorrow night it looks like.

Any special considerations for the strain? Im having good success with cubes in the fahtzip method, should be seeing pins in a day or two. Do SN/APE types need the same kind of process or something different?

Thanks!

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