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Re: Spore cleaning without a centrifuge or agar [Re: Baba Yaga] * 1
    #29133916 -

is a centrifuge necessary or is there another method to get a clean start point that you would say is adequate? I'm willing to put in the work but I don't have a centrifuge.

or if you know any vendors that you know sell spore solutions that have been cleaned with a centrifuge.


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Yummy!


LAGM 2025
https://www.shroomery.org/forums/showflat.php/Number/29174693#29174693

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Re: Spore cleaning without a centrifuge or agar [Re: syllable] * 1
    #29133928 -

Don't know why I couldn't think past a centrifuge earlier. The easiest method that doesn't require any more equipment than most people already have would be to grow fruits and print under sterile conditions. An in vitro grow would get you fruits free from contamination. Do the rest in front of a flow hood and you should be able to make clean stock solution.

I guess one could make this work in a SAB as well, at least eventually.


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🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

Edited by Baba Yaga (02/21/25 09:45 PM)

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Re: Spore cleaning without a centrifuge or agar [Re: Baba Yaga] * 1
    #29134005 -

hmmm, working on making a flowhood. it'll be a month before that happens or more. in the meantime I'll work on growing and revisit this. if all the materials used were sterilized for instance do a grow inside a jar and use sterilized sub. then print on some sterilized aluminium foil, print in a sterile petri dish.

That may be over complicating things. any advice mush appreciated.


--------------------
Yummy!


LAGM 2025
https://www.shroomery.org/forums/showflat.php/Number/29174693#29174693

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Re: Spore cleaning without a centrifuge or agar [Re: syllable] * 1
    #29134076 -



Ok so the 160f 4 hour dehydrator jars on the left. The pre cook syringe on right.

I'll give another week before tossing both. I made up syringe from a fresh print day before the make post.

At least I know my lc isn't as fucked on the same jars as these. Anyone got growth on agar yet.


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The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

Edited by vicepope (02/22/25 02:03 AM)

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Re: Spore cleaning without a centrifuge or agar [Re: vicepope] * 3
    #29134365 -

syllable said:
is a centrifuge necessary or is there another method to get a clean start point that you would say is adequate? I'm willing to put in the work but I don't have a centrifuge.

or if you know any vendors that you know sell spore solutions that have been cleaned with a centrifuge.


sporeworks

workman is the first person i ever remember talking about this. not sure if it was a trade secret sort of thing but he talked about it many years ago. i imagine he took the method from laboratory standards or research papers ect but i think it was his own sort of idea he applied to harvesting pe spores in bulk easier/more efficient than swabbing ect. i never really looked into it more than just what workman said in some posts

for posteritys sake

Penis Envy Spores
How are commercial PE syringes made?

does workman add any additional details in his recent interviews? i have not got around to watching them yet

Edited by catnip40 (02/22/25 12:15 PM)

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Re: Spore cleaning without a centrifuge or agar [Re: catnip40] * 4
    #29134376 -

Streaked a plate today with Ps cyanescens MSS, pretreated 3 hrs at ~80 *C / 176 F.

Flamed needle, spritzed a little to cool, one drop to plate, spread out with inoculation loop.

If this germinates I'll do a dehydrated gill fragment too.


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No nation, no border
Fight law and order


Species grown so far

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap] * 9
    #29134598 -

Alright, so I took two known contaminated syringes.

Tico: Wild print from 2022 I put in a syringe, white mold, bacteria, and I'm sure others. This would grow myc on BRF pucks.
PESA: Old print that was bacterial, but would grow myc on BRF pucks.

I flamed the needles and put a drop of each on 2x plates.


I took a large pot of water and brought it up to 150-170F.

Removed from bath to verify the syringes were also up to 140-160F with an IR thermometer gun.

Put them back in the bath for a half hour. Moving them and stirring the water occasionally.


Let cool in my SAB, then put a drop of each on 2x plates.


Update 3-1-2025:


Only one of the wild print Tico plates had anything show up, and it was on the pre pasteurized / dirty sample plate... and it looks like myc and something else :shrug:


The PESA did have a notable change though.

Dirty sample:


Pasteurized:


It's seemingly gotten rid of some of the contamination, but there is definitely something still there.

I need to pour more plates before I can do anything else.

Update 3-12-2025:



Dirty sample:







Pasteurized sample:


Just a checkup on these as I figure this might be useful info. The dirty samples both grew mycelium, and one of them overpowered the contamination. But there is no mycelium present in the pasteurized plates.

I ran another set of 2 syringes in the water bath and did 1hr, and 1.5hrs, nothing grew, not even on the control plates. So I'm writing that experiment off as a loss. 

I will update this comment as things progress.

Edited by Axman (03/12/25 09:45 PM)

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Re: Spore cleaning without a centrifuge or agar [Re: Axman] * 2
    #29134835 -

TIL my hot water comes out at pasteurization temperature straight from the tap.



Syringes are currently soaking in water that is 153F. Gonna go for a 3hr soak instead of 6 because I wasn't expecting it to get to pasteurization temps so quickly. I'll upload the rest of my pictures later tonight.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Spore cleaning without a centrifuge or agar [Re: Axman]
    #29134918 -

I love the stickers you're using, much cooler than my sharpie xD


--------------------
Yummy!


LAGM 2025
https://www.shroomery.org/forums/showflat.php/Number/29174693#29174693

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Re: Spore cleaning without a centrifuge or agar [Re: catnip40]
    #29134938 -

I've yet to watch them as well, no time for much of anything with finals around the corner


--------------------
Yummy!


LAGM 2025
https://www.shroomery.org/forums/showflat.php/Number/29174693#29174693

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Re: Spore cleaning without a centrifuge or agar [Re: syllable] * 1
    #29135984 -

These syringes were purchased in 2011, so this experiment will really be pushing the limits of what syringes can do.

I inoculated two plates two weeks ago. One of BHT, and the other of Golden Mammoth.

BHT



GM



As you can see, they are contam city. The BHT actually showed some white growth 1 week after inoculation, but it was intertwined with the black mold. The GM showed nothing.

I used my Ninja All in One cooker. It can PC, crock pot, air fry, and has a keep warm function. For this, I'm using the keep warm function.

I filled the pot with hot water from my tap (144F) and used a digital candy thermometer to check the temperature after about 45 minutes on the keep warm cycle. It seemed to hover between 153-154F



I put the syringes in the water for 3 hours and let them cool overnight before innoculating a single plate from each syringe with a single drop each. I did not use an inoculation loop or sterile swab, just a single drop in the center. inoculation was done inside my SAB after letting the air settle for 10ish mins.

The agar mix is 2% agar with .5% LMEA. I didn't bother to take a picture of the plates because what's the point.

I'm curious if I'll get any growth. Obviously because of the extreme age of the syringes, zero growth can not be taken as a sign that the heat killed the spores. There just isn't a way to know that there was viable spores inside the syringe to begin with.

If it does work, it will be exciting news because I have multiples of many strains that should have some less-divided genetics. Most are common varieties (amazon, B+, Cambodian, etc..), but they hav 14 less years of clone/spore cycles.

I'll post again in a week or two with results.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (02/23/25 08:04 PM)

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Re: Spore cleaning without a centrifuge or agar [Re: PuffM4gicDr4gon]
    #29136787 -

This post is gold!!!! I will try my GT print has been stored for 5 years.

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Re: Spore cleaning without a centrifuge or agar [Re: len53]
    #29136972 -

Had some delays. But the syringes I'm using provided me these jars 🤣 pasteurization will begin once our yogurt is finished lo

l.


--------------------
How I make my LC jars

Super Simple, No Rinse, Hard Boil Whole Oat Prep Tek Like a Boss: By Hamloaf
HAVING TROUBLE WITH OLD SPORES??
TRADELIST


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Re: Spore cleaning without a centrifuge or agar [Re: myceliups] * 5
    #29141539 -






Flush either didn't make spores, or made very few.  Doesn't appear albino.

Anyway, I shoved the dirty cap in a cryovial and into a cup to prevent cross contamination.

Planning to put drops on a few plates and use a cell spreader, and then several drops on a brf puck.

Looking forward to everyone's results with this.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Spore cleaning without a centrifuge or agar [Re: tree frog]
    #29142103 -

I'll have to give this a try and post the details of the experiment AFTER (not before) I get results (unlike some people on this thread - jeez!).

Anyhow, has anybody tried putting spores into a hydrogen peroxide solution? I'll give that a try as well.

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Re: Spore cleaning without a centrifuge or agar [Re: tom of alchemy]
    #29142129 -

Damn, I feel called out :lol:

Edit: Hydrogen peroxide experiments


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (03/01/25 01:37 AM)

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Re: Spore cleaning without a centrifuge or agar [Re: tree frog]
    #29142170 -

tree frog said:
Damn, I feel called out :lol:

Edit: Hydrogen peroxide experiments


Thanks for the link. I was considering putting some hydrogen peroxide inside of a spore syringe. I'm thinking about something like:

  • sterilizing a syringe
  • scraping spores into sterile water
  • drawing 90 cc of spore solution into the syringe
  • drawing 10 cc of hydrogen peroxide into the same syringe
  • inoculating agar with the spore syringe
  • wait and observe
  • post a new thread detailing the experiment and result

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Re: Spore cleaning without a centrifuge or agar [Re: tom of alchemy]
    #29142236 -

Soaking spores seems to usually kill them. But I haven't tried with different percentages. I did once try germinating spores in liquid culture and hydrogen peroxide but I can't remember what percentage I used 🤔 never worked. Im definitely curious to hear the results with your spore syringe experiment

Edited by PhenoDreamers (03/01/25 06:37 AM)

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Re: Spore cleaning without a centrifuge or agar [Re: PhenoDreamers]
    #29142489 -

SupaThaRipper said:
Soaking spores seems to usually kill them. But I haven't tried with different percentages. I did once try germinating spores in liquid culture and hydrogen peroxide but I can't remember what percentage I used 🤔 never worked. Im definitely curious to hear the results with your spore syringe experiment


That's good to know. These contams are pissing me off so much that I'm going to try anyway.

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Re: Spore cleaning without a centrifuge or agar [Re: tom of alchemy] * 2
    #29142505 -

Let's stay on topic here, please. The intention of this thread is heat pasteurization, not cultivation shower thoughts.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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