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Mikeallojee
Coolaid smile



Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
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How does sporeworks get their PE spores?
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Well, the same way anybody else does - see above replies!
But occasionally caps open enough to drop spores, I understand.
If somebody crossed PE with the odd sporeless cube, I wonder what they'd try to name it? 
Peace -PS
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Mikeallojee
Coolaid smile



Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
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I did read the relplies and they don't really answer the question. I tried to extract spores via the sqirt water into the gills method and came up with some but not much, barely visable mostly mushroom material. Yes I understand that they are very small and if you see a clump there are thousands.
But when I bought my syringe from Spore Works it had massive amount of spores in it, way more than I was able to extract from two large caps. So the question is asked again how does sporeworks get their spores? The only thing I think of is that they have a strain that does drop. If this can be verified or debunked I would appreciate it. myc
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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PE throws up fruits that look more normal and those drop spores - this has been reported often enough, particularly (possibly only) from MS cultures. Whether MS from those spores will resemble the phenotype I don't know, it likely depends on how deep the genetic barriers are.
Swabbing will possibly get you a lot more spores out, then rinsing that for the syringe might help. If I ever grow PE I'll check some of this out.
Peace -PS
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
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It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores. Even these rarely drop decent prints but the spores are there. These are gently dried, which tends to cause even more spores to mature before the cap completely dries out. The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged. Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial. Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done. This is dried and used as needed.
It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores. This method can also be used with regular cubensis caps after normal printing. There are a surprisingly large amount of spores clinging to gills. Swabbing is easier and works just as well for archiving spore samples.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
Edited by Workman (03/12/10 01:36 PM)
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Penis Envy Spores [Re: Workman]
#12191969 - 03/12/10 06:23 PM (13 years, 10 months ago) |
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Very interesting. How did you come to work out that method Workman? Or was it a pre-existing method which you found effective for working with PE?
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
Posts: 835
Last seen: 9 years, 3 months
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Re: Penis Envy Spores [Re: wisp]
#12194574 - 03/13/10 11:17 AM (13 years, 10 months ago) |
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Yeah thats really interesting, how do they keep everything sterile? as surely the top of the cap isnt sterile...
Someone who uses this method should post a video!
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
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Re: Penis Envy Spores [Re: wisp]
#12194628 - 03/13/10 11:31 AM (13 years, 10 months ago) |
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Its an adaptation of an existing method. I forget where I first read about it but I want to say Mycelium Running by Stamets. I'd have to confirm that. The method is self cleaning since the contaminates are lighter than the spores and deposit on the top of the pellet.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
Posts: 835
Last seen: 9 years, 3 months
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Re: Penis Envy Spores [Re: Workman]
#12194635 - 03/13/10 11:34 AM (13 years, 10 months ago) |
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Ahh okay, so the mushroom spores are heavier than all other contaminants, such as trich spores? What about other mushroom spores?
I really need to get my hands on that book
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
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Spores of similar size and density are going to pellet out together so I imagine spores of different species of mushrooms that are similar in size are going to be mixed. It isn't a completely foolproof method.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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houdinihar
Adventurous Learner


Registered: 09/12/08
Posts: 1,111
Last seen: 3 years, 9 months
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Re: Penis Envy Spores [Re: Workman]
#12195353 - 03/13/10 01:39 PM (13 years, 10 months ago) |
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Quote:
Workman said: It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores. Even these rarely drop decent prints but the spores are there. These are gently dried, which tends to cause even more spores to mature before the cap completely dries out. The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged. Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial. Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done. This is dried and used as needed.
It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores. This method can also be used with regular cubensis caps after normal printing. There are a surprisingly large amount of spores clinging to gills. Swabbing is easier and works just as well for archiving spore samples.
absolutely awesome Workman. thanks again for sharing your expertise to us here. always great to learn new things from pros.
houdinihar
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wisp
Registered: 04/13/08
Posts: 5,304
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I wonder how the method was first thought up? It's really quite ingenious.
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
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Re: Penis Envy Spores [Re: wisp]
#12200361 - 03/14/10 01:14 PM (13 years, 10 months ago) |
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I left out a step. You should coarsely filter the mushroom material before centrifuging. Its probably obvious that you don't need large chunks in the centrifuge.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Smartweed
Psychelica



Registered: 06/08/05
Posts: 1,033
Loc: Cloud 9
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Re: Penis Envy Spores [Re: Workman]
#12210961 - 03/16/10 03:15 AM (13 years, 10 months ago) |
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Very cool workman I will try this out!
-------------------- I believe that if people would learn to use LSD's vision-inducing capability more wisely, under suitable conditions, in medical practice and in conjunction with meditation, then in the future this problem child could become a wonderchild. - HOFFMAN My Print/Syringe Trade List
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Mikeallojee
Coolaid smile



Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
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Re: Penis Envy Spores [Re: Smartweed]
#12212740 - 03/16/10 01:31 PM (13 years, 10 months ago) |
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Thank you Workman for brining me up to speed. I had no idea it was this intensive. I will stick to swabs.
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Mikeallojee
Coolaid smile



Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
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I did the water on the gills then I poured it off into a shot glass. I did this with the whole cap and sucked all of the water out. When I was done I didn't see much in the syringe. I went to check it out a few days later and there was a shit load of spores in the bottom of the syringe where they had had a chance to settle. I haven't had a chance to inoculate anything yet but that worked really well. The syringe is loaded, unfortunately there is also some tissue in it so I wouldn't be able to send it through the mail bummer. myc
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iluvfungi


Registered: 06/17/09
Posts: 1,488
Loc: Oakland, CA USA
Last seen: 12 years, 8 months
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I think his name is almost as bad as mine. But if you can't feel me, then something is definitely wrong with you.
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Conawaaay
Stranger

Registered: 03/10/10
Posts: 44
Last seen: 12 years, 10 months
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Re: Penis Envy Spores [Re: iluvfungi]
#12268215 - 03/25/10 08:16 AM (13 years, 10 months ago) |
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woah just woah
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silas simon
get it



Registered: 03/21/10
Posts: 570
Last seen: 1 year, 10 months
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Quote:
If somebody crossed PE with the odd sporeless cube, I wonder what they'd try to name it? 
PE vasectomy variant
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ever so curious about psilocybe baeocystis. do please pm me if you can facilitate some hands on learning!
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