Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMikeallojee
Coolaid smile
Male User Gallery


Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
Re: Penis Envy Spores [Re: PrimalSoup]
    #12119719 - 03/01/10 11:43 AM (13 years, 10 months ago)

How does sporeworks get their PE spores?


Extras: Filter Print Post Top
OfflinePrimalSoup
hyperspatial illuminations
Other User Gallery


Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 1 year, 5 months
Trusted Cultivator
Re: Penis Envy Spores [Re: Mikeallojee]
    #12120460 - 03/01/10 01:40 PM (13 years, 10 months ago)

Well, the same way anybody else does - see above replies!

But occasionally caps open enough to drop spores, I understand.

If somebody crossed PE with the odd sporeless cube, I wonder what they'd try to name it? :shake:

Peace
-PS


Extras: Filter Print Post Top
OfflineMikeallojee
Coolaid smile
Male User Gallery


Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
Re: Penis Envy Spores [Re: PrimalSoup]
    #12184832 - 03/11/10 05:12 PM (13 years, 10 months ago)

I did read the relplies and they don't really answer the question. I tried to extract spores via the sqirt water into the gills method and came up with some but not much, barely visable mostly mushroom material. Yes I understand that they are very small and if you see a clump there are thousands.

But when I bought my syringe from Spore Works it had massive amount of spores in it, way more than I was able to extract from two large caps. So the question is asked again how does sporeworks get their spores? The only thing I think of is that they have a strain that does drop. If this can be verified or debunked I would appreciate it. myc


Extras: Filter Print Post Top
OfflinePrimalSoup
hyperspatial illuminations
Other User Gallery


Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 1 year, 5 months
Trusted Cultivator
Re: Penis Envy Spores [Re: Mikeallojee]
    #12188207 - 03/12/10 04:15 AM (13 years, 10 months ago)

PE throws up fruits that look more normal and those drop spores - this has been reported often enough, particularly (possibly only) from MS cultures.  Whether MS from those spores will resemble the phenotype I don't know, it likely depends on how deep the genetic barriers are.

Swabbing will possibly get you a lot more spores out, then rinsing that for the syringe might help.  If I ever grow PE I'll check some of this out.

Peace
-PS


Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery


Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
Trusted Cultivator
Re: Penis Envy Spores [Re: Mikeallojee]
    #12190289 - 03/12/10 01:35 PM (13 years, 10 months ago)

It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Edited by Workman (03/12/10 01:36 PM)


Extras: Filter Print Post Top
Invisiblewisp
Registered: 04/13/08
Posts: 5,304
Re: Penis Envy Spores [Re: Workman]
    #12191969 - 03/12/10 06:23 PM (13 years, 10 months ago)

Very interesting. How did you come to work out that method Workman? Or was it a pre-existing method which you found effective for working with PE?


Extras: Filter Print Post Top
Offlineozzysmygod
Late Night PC'ing Enthusiast
 User Gallery


Registered: 11/26/08
Posts: 835
Last seen: 9 years, 3 months
Re: Penis Envy Spores [Re: wisp]
    #12194574 - 03/13/10 11:17 AM (13 years, 10 months ago)

Yeah thats really interesting, how do they keep everything sterile? as surely the top of the cap isnt sterile...

Someone who uses this method should post a video!


Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery


Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
Trusted Cultivator
Re: Penis Envy Spores [Re: wisp]
    #12194628 - 03/13/10 11:31 AM (13 years, 10 months ago)

Its an adaptation of an existing method.  I forget where I first read about it but I want to say Mycelium Running by Stamets.  I'd have to confirm that.  The method is self cleaning since the contaminates are lighter than the spores and deposit on the top of the pellet.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Extras: Filter Print Post Top
Offlineozzysmygod
Late Night PC'ing Enthusiast
 User Gallery


Registered: 11/26/08
Posts: 835
Last seen: 9 years, 3 months
Re: Penis Envy Spores [Re: Workman]
    #12194635 - 03/13/10 11:34 AM (13 years, 10 months ago)

Ahh okay, so the mushroom spores are heavier than all other contaminants, such as trich spores? What about other mushroom spores?

I really need to get my hands on that book


Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery


Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
Trusted Cultivator
Re: Penis Envy Spores [Re: ozzysmygod]
    #12194662 - 03/13/10 11:39 AM (13 years, 10 months ago)

Spores of similar size and density are going to pellet out together so I imagine spores of different species of mushrooms that are similar in size are going to be mixed.  It isn't a completely foolproof method.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Extras: Filter Print Post Top
Offlinehoudinihar
Adventurous Learner
 User Gallery

Registered: 09/12/08
Posts: 1,111
Last seen: 3 years, 9 months
Re: Penis Envy Spores [Re: Workman]
    #12195353 - 03/13/10 01:39 PM (13 years, 10 months ago)

Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.





absolutely awesome Workman. thanks again for sharing your expertise to us here.
always great to learn new things from pros.

houdinihar


Extras: Filter Print Post Top
Invisiblewisp
Registered: 04/13/08
Posts: 5,304
Re: Penis Envy Spores [Re: houdinihar]
    #12196252 - 03/13/10 04:54 PM (13 years, 10 months ago)

I wonder how the method was first thought up? It's really quite ingenious.


Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery


Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 3 hours, 42 minutes
Trusted Cultivator
Re: Penis Envy Spores [Re: wisp]
    #12200361 - 03/14/10 01:14 PM (13 years, 10 months ago)

I left out a step.  You should coarsely filter the mushroom material before centrifuging.  Its probably obvious that you don't need large chunks in the centrifuge.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Extras: Filter Print Post Top
InvisibleSmartweed
Psychelica
 User Gallery


Registered: 06/08/05
Posts: 1,033
Loc: Cloud 9
Re: Penis Envy Spores [Re: Workman]
    #12210961 - 03/16/10 03:15 AM (13 years, 10 months ago)

Very cool workman I will try this out!


--------------------
I believe that if people would learn to use LSD's vision-inducing capability more wisely, under suitable conditions, in medical practice and in conjunction with meditation, then in the future this problem child could become a wonderchild. - HOFFMAN

My Print/Syringe Trade List


Extras: Filter Print Post Top
OfflineMikeallojee
Coolaid smile
Male User Gallery


Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
Re: Penis Envy Spores [Re: Smartweed]
    #12212740 - 03/16/10 01:31 PM (13 years, 10 months ago)

Thank you Workman for brining me up to speed. I had no idea it was this intensive. I will stick to swabs.


Extras: Filter Print Post Top
OfflineMikeallojee
Coolaid smile
Male User Gallery


Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 10 years, 11 months
Re: Penis Envy Spores [Re: Mikeallojee]
    #12251339 - 03/22/10 08:33 PM (13 years, 10 months ago)

I did the water on the gills then I poured it off into a shot glass. I did this with the whole cap and sucked all of the water out. When I was done I didn't see much in the syringe. I went to check it out a few days later and there was a shit load of spores in the bottom of the syringe where they had had a chance to settle. I haven't had a chance to inoculate anything yet but that worked really well. The syringe is loaded, unfortunately there is also some tissue in it so I wouldn't be able to send it through the mail bummer. myc


Extras: Filter Print Post Top
Offlineiluvfungi
Male User Gallery

Registered: 06/17/09
Posts: 1,488
Loc: Oakland, CA USA Flag
Last seen: 12 years, 8 months
Re: Penis Envy Spores [Re: Mikeallojee]
    #12266964 - 03/24/10 11:35 PM (13 years, 10 months ago)

I think his name is almost as bad as mine. But if you can't feel me, then something is definitely wrong with you.


Extras: Filter Print Post Top
OfflineConawaaay
Stranger
 User Gallery
Registered: 03/10/10
Posts: 44
Last seen: 12 years, 10 months
Re: Penis Envy Spores [Re: iluvfungi]
    #12268215 - 03/25/10 08:16 AM (13 years, 10 months ago)

woah
just woah


Extras: Filter Print Post Top
Offlinesilas simon
get it
 User Gallery


Registered: 03/21/10
Posts: 570
Last seen: 1 year, 10 months
Re: Penis Envy Spores [Re: PrimalSoup]
    #12273770 - 03/25/10 11:35 PM (13 years, 10 months ago)

Quote:

If somebody crossed PE with the odd sporeless cube, I wonder what they'd try to name it? :shake:



PE vasectomy variant


--------------------


ever so curious about psilocybe baeocystis. do please pm me if you can facilitate some hands on learning!


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Experience with long-term storage in distilled water?
( 1 2 all )
ragadinks 7,103 26 10/25/03 09:16 PM
by Cow Shit Collector
* Re: any one with even pin set please respond Anonymous 947 7 04/23/00 01:02 PM
by WildLASER
* Can DI water be used for spore syringes. *DELETED* OOKLA 1,142 8 09/13/03 01:21 PM
by ExtravagantDream
* lots of spores Wapo 1,991 12 06/22/03 08:59 PM
by Raadt
* Homemade Glovebox? Minimum CFM??? socratesmind 3,727 15 09/24/02 12:51 PM
by socratesmind
* glovebox q and q K0r 1,178 7 03/15/02 11:37 AM
by BeppoMarx
* Honey water spore syringes
( 1 2 all )
flawed 5,543 30 11/15/02 02:54 PM
by DrJoseph
* Does H2O2 KILL spores or just inhibit germination?
( 1 2 all )
micro 7,070 37 11/26/03 09:24 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
6,789 topic views. 1 members, 4 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.005 seconds on 13 queries.