catnip40 said:

RoscoeReturns said:

catnip40 said:

how far do you reckon this goes in the other direction? spores surely last below freezing right?

I've heard people mention that cubes dont exist outside their native range not because of the cold winters/weather its because outside the gulf/native range theres not enough moisture for them to exist. but in reality it prob has to do with both of those equally

i'm not sure i have ever actually tried to find out what temp or how long at "what" temp a cube spore dies though and i dont really think theres any actual information on that either lol

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I have no experience with spores, but cubensis mycelium survives freezing for extended periods. I’ve revived cubensis mycelium that was in the freezer for a year. I would think spores would be even more cold hardy. I guess my question is what do you gain by freezing spores? I understand the utility of pasteurized spores when trying to save a contaminated culture, but freezing?

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dont know, dont care!! just popped into my head, i told stipe i wasnt trying to derail the thread.

there are some papers about freezing cultures and or spores to deal with mites but i think heat might prove more beneficial for that as well

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RoscoeReturns said:

I have no experience with spores, but cubensis mycelium survives freezing for extended periods. I’ve revived cubensis mycelium that was in the freezer for a year. I would think spores would be even more cold hardy. I guess my question is what do you gain by freezing spores? I understand the utility of pasteurized spores when trying to save a contaminated culture, but freezing?

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Thank you for this. I've been trying to find a way to preserve semperviva spores for longer than a year to no avail, unlike the mycelium that can be kept in stasis for 2+ years (in my own experience) and brought back to life without a problem (well, not really hibernating, are they? lol)

Case in point:



If we can preserve the integrity of such 'short shelf life' stubborn spores for at least 10 years, we'll be golden

ps,
Apologies @Stipe, not trying to derail the thread or anything, just adding more food for thought

Stipe-n Cap said:

I've proven the concept for cube spore survival at 160°F, at least (hyperlinked above). We know that vegetative bacteria, yeasts, and molds die at pasteurization temperatures, obviously, otherwise pasteurization wouldn't work; so, with these basic facts combined it seems reasonable that we could get away with it. Dropping the temp down from 160°F to 145°F should provide a greater likelihood of active spore survival, in any case.

145°F for 60 mins will be my recommended basiline, with adjustments made if necessary.

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Stipe-n Cap said:

Keep in mind that we don't know how many of our spores die off when exposed to those temps. That's why I suggested an initially short exposure, and low temperature. To maintain heterozygosity with wild spores this will definitely be a consideration.

So, what we should be focusing on is who can get the best results at the lowest temp and exposure time. I wouldn't go lower than 30 mins, but 1 hour seems reasonable.

All data points are valuable so even if someone chooses longer exposure at higher temps, the results will help plot and dial our preferred outcome.

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Bigworm said:

What about a moistened spore swab in vacuum sealed bag in a sous vide bath at 165f? Or would I want to go higher temps to get to 165f?

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Stipe-n Cap said:

The objective is to destroy heat sensitive  vegetative competitor organisms while inflicting minimum damage on our active spores.

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Stipe-n Cap said:

I'm all ears if you have any suggestions.

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