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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: suicideprevention] * 4
    #29116361 -

suicideprevention said:
Can someone point me in the direction of a link for preparing bulk with coir and verm?  I have built a tub and have 3 quarts of spawn ready to go to bulk this weekend. I also have coir and vern.  Do I boil water and mix it with coir? mix it with coir and verm?  Ratio of coir to verm to water to spawn, etc.  Is there a tek that lays this out?


Welcome, here you go. The hitchhikers guide covers all the basics.
https://www.shroomery.org/forums/showflat.php/Number/28501530#28501530

This is directly to the coir prep.
https://www.shroomery.org/forums/showflat.php/Number/28507557#28507557


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29116484 -

ThurmanMurman said:
If a culture is being refrigerated on agar in a holy grail, would it be advised to still leave the cap cracked so it can breathe or tighten it down so it dries out slower*. I figured if we're storing genetics in an air tight cryovial, killing it outright probably isn't a concern?


I'm not quite familiar with these... are the lids threaded? If not wouldn't leaving cracked present contamination problems?

But if in the refrigerator the mycelium is practically dormant and I'd assume is fine to close.  Since most slants are sealed for stasis as well.

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Re: Ask Quick Questions, Get Quick Answers [Re: Studebaka] * 2
    #29116487 -

As long as it was clean clean clean.... the moisture is not that big of problem but should be avoided.

What did you follow for to prep grains?

Grain spawn is probably the biggest/most important aspect of this hobby. And preparing clean, properly hydrated spawn is essential but takes practice and diligent note Taking for repeatability.

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29116500 -

ThurmanMurman said:
If a culture is being refrigerated on agar in a holy grail, would it be advised to still leave the cap cracked so it can breathe or tighten it down so it dries out slower*. I figured if we're storing genetics in an air tight cryovial, killing it outright probably isn't a concern?


Close them up , if u have an Insulated lunch kit it will help with temp swings and condensation


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29116518 -

If my main goal for a culture on my plate is strictly to get it as clean as possible. If this is achieved on the first transfer is there any point in making more transfers?

I'm a little confused I'm just trying to figure out when I should be taking my next transfer.. like the first transfer taken from The Germ plate is taken from the fastest Leading Edge as soon as you have growth.

Here's where I'm confused From this point on If you take a transfer that early What if the plate grows out and ends up being clean and transfer was unnecessary?

So I guess I'm wondering after T1 do we only go for the next transfer when we see visible contamination? Otherwise we just leave it be?

All these plates I'm about Post are T1 And I'm trying to figure out when they're ready for T2 Or If T2s are even necessary at this point. Other than the desire to achieve Rhizo which has no actual effect on the grow right?

I know it's still hard to tell because it's so early on. But that's part of the question. do I let them  grow more And see what may show up  first?

My current number of usable dishes with agar ready at the moment are kind of limited so I'm wanting to only take transfers and use them as absolutely necessary.









I can post backlit pictures if necessary. Didn't know if y'all needed that to answer my question or not. So I just went without it to start.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 3
    #29116520 -

If a plate is clean then the only reason to transfer further is to maintain the culture for the future.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 1
    #29116528 -

Clean is clean we only need to expand to save the culture or expand for more jars :super:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 2
    #29116530 -

Awesome.  That's what I had figured.

Thanks for the extremely fast responses.
:rockon:


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29116531 -

Can air bubbles somehow show up in agar months after it was prepared and put into dishes? You see in that post of all my dishes. the very last green plate has two little air bubbles right about at 10:00 on the outer edge of the culture. I swear I don't remember those being there before. I think they just showed up over the past couple days. I'm so confused. LOL I mean. can contamination show up as air bubbles? Has anybody ever seen this before?
Let's say they weren't there before and they did just show up. How?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29116535 -

if they just showed up its probably bacteria but it does just look like a couple bubbles to me


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29116640 -

callmehippie said:
Has anyone tried the "buffered" coco bliss.




As opposed to coco bliss "premium coco coir pith"



I never noticed they had one that was buffered.


This is what buffered means


100% Buffered Coco Coir for Plants - Our coco coir block has been pre-buffered to cut down excess salts and stabilize pH levels, saving you the time and hassle of buffering it yourself. This enhances nutrient availability and soil balance, making Buffered Coco Bliss an ideal soil amendment for indoor plants, outdoor plants, and more.

Does it make a difference?


Buffered I believe is supplemented with calcium. It's pH buffering is what they are talking about. Coconut coirs base pH is around 5.

I'm pretty sure they wash the salt out of both.

As far as how much difference any of this makes, it kind of depends on if you are using a proper casing or not. High salt content as well as low pH can interfere with pin formation. But that really only matters on the surface. And both of those matter more with less domesticated species than cubes.



Mystery SA 'cased' with pH adjusted coir (calcium catb and gypsum, but I buffered it myself).


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29116744 -

I have a few spore swabs that I procured about nine months ago but couldn’t use earlier due to personal reasons. Recently, I prepared agar using 3.5g of agar in 250ml of water, with a pinch of manure and grain water. This is the medium I typically use for propagating agar transfers and cloning.

For germination, I swiped the swab across the agar plate and also placed a small piece of the swab in the center of the plate. However, in my previous attempts, nothing germinated even after two weeks. What eventually worked for me was placing the swab piece in a liquid culture (LC), allowing it to germinate, and then cleaning it up through multiple agar transfers.

Most sources online suggest that simply swiping the swab on agar should be sufficient for germination. Am I missing something? Has anyone else experienced this issue? I’d love to hear your methods for successfully germinating spores from a swab onto agar.


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Happy growing and may your harvests be plentiful! 🍄✨
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Re: Ask Quick Questions, Get Quick Answers [Re: MalluShroomer] * 1
    #29116751 -

My first swab attemp on agar produced nothing but bacteria. You could see spores left behind but they would not germinate.

I had to put my swab pieces in BRF pucks.

Search pristine brf pucks

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Re: Ask Quick Questions, Get Quick Answers [Re: aShogunNamedMarcus]
    #29116754 -




Should I pick these 2?
And are they legit although the fuzzy feet?

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Re: Ask Quick Questions, Get Quick Answers [Re: killedSanta] * 4
    #29116766 -

How do they feel? They look close to ready.

Don’t worry about things like “fuzzy feet.” That’s not fuzzy feet. Plenty of varieties just get wooly at the base. Has nothing to do with health. Fuzzy feet is a condition resulting from too little oxygen, and the mushroom grows extra surface area to try and get more. You’ll know it when you see it, because it looks kinda wacky.

Even still that wouldn’t make your mushrooms inedible or bad.

Here’s what you want to not see:



--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29116770 -





Would you open the Bag and transfer to a Shoebox ore let the side pins grow?

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet] * 1
    #29116778 -

I’d just let that do its thing. It’s already going 🤷‍♂️


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #29116792 -

:whathesaid:


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29116793 -

Smellyhobbit said:
I’d just let that do its thing. It’s already going 🤷‍♂️



OK my friend :laugh:

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet] * 2
    #29116797 -

You're finally getting shrooms to grow and want to change?

Take a breath... relax... watch the magic happen!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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