Dkeppel said:

Asura have you managed to find some time to put bbar to agar?

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tree frog said:

Also depends on how big your plates are. If you're taking a bunch of fast transfers to 60 mm plates or little takeout cups that's different than letting a 90 mm plate grow out completely.

I just moved over to 90 mm myself and what I've been doing is taking a quick transfer off of my germ plate and putting three wedges on that first 90 mm plate.  So my t1 I'm not even growing out 60 mm.  Because my t1 plates have three wedges on them.

And if the growth looks good I don't even let it grow out very far before transferring it by itself to a 90 mm.  If that second transfer has nice radial growth I inoculate from there.  And usually I'm making enough transfers off of my germ plate doing it this way, like six to nine, the I'm going to get a couple of clean plates off of it assuming I'm not working with dirty or wild prints.  And if that's the case I would re-terminate and probably cabin sequester everything on the t1 or germinate on BRF pucks.

So, long story short. It's not so much an issue of what transfer you are on but how much you are letting them grow out on the plates.

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PuffM4gicDr4gon said:

Hey Panatics! I just put some Australian Red Down Under to a t2 transfer. I picked out the fastest and whispyist growth and made about 10 plates. I'm going to let those grow, and select the fastest/whispyist of those.

I've seen several mentions about Pans not being worthwhile going past T2. Is that related to growth type, or overall cleanliness of the culture? Like if some of the T2s don't have clean or even growth, is it worth taking them to T3 to clean them up?

I tried some BVI last year and failed because I was still learning agar and how to spot healthy cultures.

I'm just trying to understand if I have to hope/pray that the t2s come out clean and start over if they don't, or if I can clean them up as I would a cube culture?

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That would be T1, not T2 my friend. Just needed to clarify that for the sake of fellow members reading

tree frog said:

Also depends on how big your plates are. If you're taking a bunch of fast transfers to 60 mm plates or little takeout cups that's different than letting a 90 mm plate grow out completely.

I just moved over to 90 mm myself and what I've been doing is taking a quick transfer off of my germ plate and putting three wedges on that first 90 mm plate.  So my t1 I'm not even growing out 60 mm.  Because my t1 plates have three wedges on them.

And if the growth looks good I don't even let it grow out very far before transferring it by itself to a 90 mm.  If that second transfer has nice radial growth I inoculate from there.  And usually I'm making enough transfers off of my germ plate doing it this way, like six to nine, the I'm going to get a couple of clean plates off of it assuming I'm not working with dirty or wild prints.  And if that's the case I would re-terminate and probably cabin sequester everything on the t1 or germinate on BRF pucks.

So, long story short. It's not so much an issue of what transfer you are on but how much you are letting them grow out on the plates.

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Same here yayy, i love those 90mm plates, they're really nice

Regarding pan transfers, i do let the mother plate grow enough (7 days worth of growth, that is) to cherry pick the best looking spots which isn't that difficult if you're used to how they express themselves on agar. Pan Bispo proved a bit of a challenge when the mycelium turned out light gray and very thin..almost mold like. Semps on the other hand, those do get 6 or 7 T1s and with good reason, myceliated grain jars are next level treasure. The thing with pan cyans i learned through the years, they do well with a couple of jars colonizing per pan cyan variety, i.e. 2 jars for Peace River, 2 jars for Texas maybe 1 jar for TTBVI and so on. They run very well around 1:3~1:4 in my experience. That being said, growing conditions will differ for everyone and what they can work with. I'm an agar maniac so its a lot more agar work than actual growing and for that i'm rightfully guilty as charged lol

ps,
Love your work, sister