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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 3
    #29064980 -

Received this from vabiooth..


Seems it’s been happening to others as well so beware if you got the 90mm recently.. some of them might melt

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #29064996 -

At least they're willing to reimburse.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja] * 7
    #29065002 -

I told them I wasn’t seeking a refund but I do want the ones that melt replaced.  I don’t want possibly contribute to the only autoclavable plastic petris going away lol

I asked if I could contact them at a later date when/if I have a decent amount that melted, getting those replaced with 60mm all at once.  Also asked to keep me informed with what their investigation finds.  I think there was just a mistake made somewhere with a batch.  They asked about the packaging because they also produce non-autoclavable petris but based on the video on Amazon, it’s a pretty automated system so who knows.  I’ll keep ppl posted

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #29065164 -

fahtster said:
Received this from vabiooth..


Seems it’s been happening to others as well so beware if you got the 90mm recently.. some of them might melt



Glad to see that they seem to be responsive. I have run the 60 and 90 mm many cycles without any troubles. Vabiooth sells both Autoclavable and disposable petri dishes in the 90mm size. I wonder if some got mixed up somewhere along the way.

I don't think they are intentionally scamming since I have literally hundreds of plates that have gone though many cycles without any warping. But clearly someone messed up somewhere with those.

Hope they get you sorted out.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 4
    #29065180 -

fahtster said:
I told them I wasn’t seeking a refund but I do want the ones that melt replaced.  I don’t want possibly contribute to the only autoclavable plastic petris going away lol

I asked if I could contact them at a later date when/if I have a decent amount that melted, getting those replaced with 60mm all at once.  Also asked to keep me informed with what their investigation finds.  I think there was just a mistake made somewhere with a batch.  They asked about the packaging because they also produce non-autoclavable petris but based on the video on Amazon, it’s a pretty automated system so who knows.  I’ll keep ppl posted



Received this email today.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Henry Swanson] * 3
    #29065191 -

Oh yeah, don’t think anyone is cramming anyone and they’re being super cool.  I do think it was one of the 10 packs I initially bought and not the 100 as the box clearly says it’s the autoclavable kind.  The second 10 pack I got came like weeks before it was suppose to because they were out.. it just showed up and I thought oh cool and didn’t pay much attention to the packaging because it looked like the first one.  I also only got one 10 pack when that order was suppose to be 30.. I cancelled the other 20 and said fuck it and ordered a 100 box lol. So in hindsight, that’s suspicious. 

It was probably on amazons end I’m assuming

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Henry Swanson] * 3
    #29065197 -

Henry Swanson said:
fahtster said:
I told them I wasn’t seeking a refund but I do want the ones that melt replaced.  I don’t want possibly contribute to the only autoclavable plastic petris going away lol

I asked if I could contact them at a later date when/if I have a decent amount that melted, getting those replaced with 60mm all at once.  Also asked to keep me informed with what their investigation finds.  I think there was just a mistake made somewhere with a batch.  They asked about the packaging because they also produce non-autoclavable petris but based on the video on Amazon, it’s a pretty automated system so who knows.  I’ll keep ppl posted



Received this email today.




Puts pitchfork away
:skol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Jebi] * 8
    #29066171 -

Just to put everyone’s mind at ease that may be worried, after thinking about the steps of what happened, I’ve come to the conclusion that the second 10 pack of plates I ordered weren’t actually vabiooths autoclavable dishes.  I inspected the first box I received because it was a new product to me and checked everything out.  After using them, I quickly figured out 10 dishes isn’t going to be nearly enough so I ordered 30 more

Well on the day they were to be delivered, I got a notification that it was going to be pushed out 2 weeks.. then a few days later, a single 10 pack showed up without notice.  I thought “cool” and not “weird” which is what I think now in hindsight.. well I cancelled the other 20 and ordered the 100 box.

I didn’t look at the box printing at all since it looked just like the first box essentially (just a brown box) and used the plates and threw away the box.  Odds are that someone Amazon mixed up the non autoclavable type when they ran out of the autoclave ones.  So if your box had/has the company printing on them that says it’s autoclavable, you’re fine.

Sorry about the scare

I pc’d 5 more and one of them melted so that brings it up to 6 I’m expecting to find 4 more at some point

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #29066606 -

LC tests - what do you think? TTBVI and machine elf looks sus right? apart from that id send it. All from the 18.12.24. Was just dreaming a bit while labeling


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Re: AGAR ENVY! (Anything and All things agar!) [Re: tittlez]
    #29066649 -

Is fluffy agar a sign of bacteria or other contamination?





All seem to be from my Nat plates. Just trying to figure if I should do more transfers.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PuffM4gicDr4gon] * 1
    #29066651 -

Was going to ask if those were nats until I got to the bottom of the post. Nats just kind of do that. They can be fluffy and aerial on agar. Still might want to take 1 more transfer though. Maybe lower your nutes in next batch if not already at 1.5%


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #29066654 -

AspectOfTheCreator said:
Was going to ask if those were nats until I got to the bottom of the post. Nats just kind of do that. They can be fluffy and aerial on agar. Still might want to take 1 more transfer though. Maybe lower your nutes in next batch if not already at 1.5%



Will do! The plates should be around 1%. Thanks for taking the time to reply


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PuffM4gicDr4gon] * 5
    #29069283 -

LAGM 2025 agar prep, part 1:


15x PDA plates ready to go. And since I used a french press for the potato water, they are crystal clear!

Will be doing 12-15 LME plates next.


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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: AGAR ENVY! (Anything and All things agar!) [Re: KlarnEvil9] * 4
    #29069386 -

BC Nat plate



Not a BC Nat plate


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FuzzyFoot]
    #29069741 -

Thats COOL AF!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja] * 1
    #29072779 -

Hello all, I need to up my posts so I will share some plates I have been working with lately. Does anyone her have any success with this kind of trenching method? I think it would be helpful for explicitly bacterial cultures but mold and myc mixed together I am not so sure.


Here is a Yeti plate that has what I believe to be a mold colony at 6 oclock but promising transferable edges most everywhere else.



Here is what I am talking about highlighted.



Here is a rusty whyte culture that I trenched. Originally I had trenched it because the germ plate and T1 looked rough, now that it has grown out I wonder If it did it any good. Looking up at the top of the plate It looks like a clean transferable edge, very comparable to the myc that crossed the trench.



Anyway, do any of you use this method, or is the sandwich method more popular for dirty cultures.


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We stand on the shoulders of giants


Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mids] * 2
    #29072784 -

If mold is enmeshed, the plate is probably fucked. :frown: I'd transfer from furthest away from the weird growth on the chance it's really localized.

The best method for cleaning up bacterial cultures in my humble opinion is the BRF puck.

Edit to add that what you observed with that RW plate is how most of my trench experiments have gone. Probably because the culture wasn't bacterial to begin with but was growing weird during recovery.

Edited by Phaethon (12/30/24 09:30 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #29073083 -


the way the leading edge at 9 o'clock looks...Is that what im looking for around the entire circle?  Should it be taking off like like evenly around entire edge?  Just keep taking samples of those leading edges until I have even leading edges??  Can I take just a piece of that 9 o'clock leading edge and make LC out of it?  Looks like very clean sample?

thanks

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Re: AGAR ENVY! (Anything and All things agar!) [Re: chfbrd]
    #29073084 -

just zoomed in and noticed its not very clear...I will try and get better, clearer pic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: chfbrd]
    #29073101 -

From what I can see in the pic, 3-5 and 7-12 seem nice, 5-8 seems funky and 9 might be a reaction (aerial rhizo) to 5-8. Pic isn't clear enough though

Says dr shitpics

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