Churpee Choozle said:

I really like Josex' posts, and have been saved more than once by the Josex poke. And yes, I have been raised with the endospores too, everything that went wrong was always endospores (while shooting entire sporeprints in jars of wet grain, lol). I was just taken aback by his belief in them at the time he started that thread. And I guess even more by the notion that clear LC is by definition clean while agar is hard to judge. It's cool to see how young the current status quaestionis is...
I feel stupid for not seeing the big picture with GWA, having read Rachel Carson (and her ideas on the accumulation of toxins) and having fiddled around with Claviceps / Gloeotinia a lot in the past ('discovering' the astounding amount of species that live in a single ear of grain).
Thanks for sharing man. It was a really interesting read.

I am really confused as to what I think of GWA now. Especially since I used it for LAGM 25. The problem with the subaeruginosa is probably just the culture itself. I have ovoids and IWL on the exact same batch of agar and they're fine. Just to be sure, I'll put the subs on different agars tonight.

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haniblecter said:

Churpee Choozle said:

I really like Josex' posts, and have been saved more than once by the Josex poke. And yes, I have been raised with the endospores too, everything that went wrong was always endospores (while shooting entire sporeprints in jars of wet grain, lol). I was just taken aback by his belief in them at the time he started that thread. And I guess even more by the notion that clear LC is by definition clean while agar is hard to judge. It's cool to see how young the current status quaestionis is...
I feel stupid for not seeing the big picture with GWA, having read Rachel Carson (and her ideas on the accumulation of toxins) and having fiddled around with Claviceps / Gloeotinia a lot in the past ('discovering' the astounding amount of species that live in a single ear of grain).
Thanks for sharing man. It was a really interesting read.

I am really confused as to what I think of GWA now. Especially since I used it for LAGM 25. The problem with the subaeruginosa is probably just the culture itself. I have ovoids and IWL on the exact same batch of agar and they're fine. Just to be sure, I'll put the subs on different agars tonight.

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Ive been deep diving into this post, can someone define the acronyms used? searching and the two acronym threads I have bookmarked are not helping.

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haniblecter said:

not too early, but it'd be hard to not beat up the myc too much with a scapel...assuming you use a scapel.

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gibilan11 said:

Hey! Why is the myc on these plates growing in a "ring"-like pattern with inconsistent density?



The only thing I could think of is the fact that I've been away for a few days during the holidays and the temperature may have dropped to a few degrees lower than usual (17°C instead of the usual 21-22°C). These are Nats, T2 6 plates and they're all more or less in a similar pattern. T1 from WA seemed more uniform.

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wichita_jack said:

I have these plates from two LCs...SNAPE and a Tidal Wave x APE cross.

This is my first time getting LC to take on agar...I had only done clones and clone transfers previously.

Finally just went ahead and made my own plates, not as hard as had feared and thus far better looking results.

Couple quick questions...

How do these look for taking transfers?

Where on each are the best spots to transfer from?

Appreciate any help.



Also having a terrible time getting formatting down on this site...any teks for that?!?

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Phaethon said:

wichita_jack said:

I have these plates from two LCs...SNAPE and a Tidal Wave x APE cross.

….

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Generally, take small transfers from the leading edge of nice uniform-looking growth. Some ideas:
- SNAPE 1: most anywhere from center colony, 2 o'clock from upper-right colony
- SNAPE 2: needs more time
- TWxAPE
  - 1: 7-10 o'clock center colony
  - 2: probably toss, but could try 4 o'clock bottom colony
  - 3: lots of sus, maybe top colony, 3-5 o'clock
  - 4: looks uniformly sus but could just be condensation, take your pick

Those align=left properties on your img tags were wreaking havok (I removed them in my reply). Just copy the bbcode directly from the pics overview. Don't try to force a particular layout and you and everyone will be happier.

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bluespirit13 said:

Hey, I just did some of my first transfers and I had some unexpected issues pop up so I just wanted to ask some questions.

I used Pasty Plates made in Glad Mini Round containers and I followed the tek exactly.

Before PC, the lids kinda snapped on. After the PC it seems the lids are no longer snapping on and they seem to lift right off the container with ease. I didn’t see anyone else talking about this issue so I’m trying to figure out where I may have went wrong.

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bluespirit13 said:

Yeah I hit it with some micropore tape. Just seemed weird since I’ve seen plenty of people use them without issue. It’s like they don’t seal at all anymore after PC. Idk I think im gonna have to look for a different way to do agar.

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