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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29064407 -

Hey guys, first time grower here.

I have a tub that hasn’t fully colonised but have noticed a few pins have shown up.
Are these likey ‘aborts’?
Should I start fruiting this container? It’s going on 3wks now and the sides look good but the top is yet to 100% colonise?
The spawn mixture was a millet and manure substrate all in one bag.
Once bag was 80-100% colonised I broke up and transferred to monotub mixing coir/peat into substrate. Then top layering with a thin layer of coco coir/peat. (starting to think I didn’t need to add the coir/peat). 
Then sealed up and unopened for roughly 2wks. Top substrate looked a lil dry so I misted lightly. Went to check tub last night and noticed these pins poking through!
Was trying to get 100% colonisation before fruiting but should I start fruiting this mono?




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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29064437 -

Quote:
Ah yes. Indeed. Pff. And then actually 8g of agar sounds a bit low to me :smile:.


I've done 8 before and it's worked for me. Depends on the brand though, some brands can be harder others more gelatinous. I went down to 8 with some hard to germinate spores at one point and it did the trick, I'm guessing more available moisture. I was considering 8 to help rehydrate those 20 year old spores I have but I may just swab and impregnate a brf cake because that sounds more fun tbh 🤣


--------------------
15 year lurker

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile]
    #29064442 -



What type of contamination is this? Never seen this before.

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29064447 -

cybork said:
LewDoja said:
I think that's  a decent recipe...  I've never strained it before. And it doesn't fully dissolve so you might want to add a touch more (1.25g total)
though it's not necessarily going to help with old spore germination.  The pressurized syringe might be worth trying if the regular route doesn't work out.

I've had 10yr+ spores on agar germinate with a hot pour (about the same consistency as this recipe).

Is there a reason you're wanting to change?


So, based on what Meanwhile said, 14% of LME sounds good? 14% sounds like very much, but I just fuck around really. And I haven't revived anything older than a year yet. But I'm interested in if so, what are the benefits of higher percentages than I do now.

I also use 4% agar by the way. 8gr = 16%. Sounds like way too much. But I can be wrong there as well.

This is what Meanwhile mentioned:
Meanwhile said:
(...)
500ml of dh20
7g lme
8g living jin agar
0.5g of nutritional yeast(boiled for 5 minutes then strained in 100/500ml of water)
(...)





500×2%=10

500×1.6%=8

500×1.4%=7


Edit:
Quote:
It'd be 1.4% no?


Quote:
Ah yes. Indeed. Pff. And then actually 8g of agar sounds a bit low to me :smile:.



Yes it is a little soft.  Wouldn't want to go much lower. But that is a decent  brand of agar. Which should well for spore germ. 
Higher than 2% gets difficult for stubborn spores but can be a life saver for hard to clean bacterial plates.

Edited by LewDoja (12/22/24 05:35 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca] * 1
    #29064463 -

KauaiOrca said:
If I was to store spores in a sealed medical vial suspended in water, how long would they likely be good for?  And should they be kept at room temp or in the fridge?



It would be best to have a dedicated fridge for keeping constant Temps and reducing possible contamination. Shelf life is probably longer than 5 yrs barring any freezing or temp malfunctions.

If your spore solution is sterile you're likely able to keep 2-5+ yrs at room temp maybe longer.

Avoid light

Edited by LewDoja (12/22/24 05:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: bongoman]
    #29064467 -

Hard to tell... other than looks like mold

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile]
    #29064471 -

Meanwhile said:
Quote:
Ah yes. Indeed. Pff. And then actually 8g of agar sounds a bit low to me :smile:.


I've done 8 before and it's worked for me. Depends on the brand though, some brands can be harder others more gelatinous. I went down to 8 with some hard to germinate spores at one point and it did the trick, I'm guessing more available moisture. I was considering 8 to help rehydrate those 20 year old spores I have but I may just swab and impregnate a brf cake because that sounds more fun tbh 🤣


I'd do both the brf and stick the swab in the soft agar (maybe swipe around on another plate first. Especially if it's likely to be dirty.)

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Re: Ask Quick Questions, Get Quick Answers [Re: LobstaBob]
    #29064479 -

Well it's fruiting already so I'd put it in fruiting condition (most ppl don't differentiate and straight to FC after spawn.) 
But your scenario makes sense as to why you're seeing pins the perfect conditions to FORCEFULLY trigga' fruiting
(high co2 --> drastic decrease of CO2 ++ dry sub --> not dry sub =increased humidity/FAE )

The peat is probably to variable that is giving you appearance <100%

Edited by LewDoja (12/22/24 06:00 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29064578 -

There was a thread around here somewhere about micro pore tape, and somebody took a microscope and looked at it to show you how big the holes in it were.

Does somebody have a link for it?


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29064586 -

why


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29064587 -

I wanna use it for a write up.
When I initially made it, I recommended mpt. Then I heard about that thread and saw it at some point. The pictures were devastating.

I want it to be linked in that place that I initially suggested it to dissuade people from thinking it’s acceptable anymore.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

Edited by GenesisCorrupted (12/22/24 08:52 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29064610 -

LewDoja said:
KauaiOrca said:
If I was to store spores in a sealed medical vial suspended in water, how long would they likely be good for?  And should they be kept at room temp or in the fridge?



It would be best to have a dedicated fridge for keeping constant Temps and reducing possible contamination. Shelf life is probably longer than 5 yrs barring any freezing or temp malfunctions.

If your spore solution is sterile you're likely able to keep 2-5+ yrs at room temp maybe longer.

Avoid light


Just out of curiosity, how do you think a sealed medical vial in the fridge could get contams inside?


--------------------
“Order is needed by the ignorant but it takes a genius to master chaos.”
― Einstein


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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca]
    #29064648 -

Anyone ever have issues venting their pc? IE I've been trying to vent this freakin thing for the past 2 hours. The locking mechanism works and pops up, it's spewing hot air, but for some reason I can't get a solid stream of steam. I have plenty of water in there. I have it on the hottest setting for my stove. It's like this thing has a mind if it's own all of a sudden.


--------------------
15 year lurker

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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca]
    #29064649 -



Accidentally set the dehydrator at 180. They were also pretty coated in spores - black from spore load or burned? Im leaning toward burned...are they salvageable and just ugly? I can't believe I did that...🤦‍♂️


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29064651 -

https://www.shroomery.org/forums/showflat.php/Number/28749207#28749207
https://www.shroomery.org/forums/showflat.php/Number/27761725#27761725

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29064653 -

Spore load...

I usually wipe with damp paper towel when heavily spored

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile]
    #29064655 -

Maybe turn it down a little bit once it starts ... not much to it, only trying to to displace all the air with steam, so after 20 min max it should be ready to increase pressure

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29064659 -

ThurmanMurman said:


Accidentally set the dehydrator at 180. They were also pretty coated in spores - black from spore load or burned? Im leaning toward burned...are they salvageable and just ugly? I can't believe I did that...🤦‍♂️


I once air-fried my mushrooms at like 400F, needless to say mine were not salvageable, and looked worse. I've had heavy spore loaded caps look the same when dehydrated. You're probably good.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29064662 -

LewDoja said:
Spore load...

I usually wipe with damp paper towel when heavily spored


Yeah, they do be ugly, but they'll go in capsules anyway, so no harm really. Thanks amigo - I was a little worried bc they almost smelled burnt, but coulda been something else in there. Wouldn't hurt to clean it. Lol


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29064663 -

Axman said:
ThurmanMurman said:


Accidentally set the dehydrator at 180. They were also pretty coated in spores - black from spore load or burned? Im leaning toward burned...are they salvageable and just ugly? I can't believe I did that...🤦‍♂️


I once air-fried my mushrooms at like 400F, needless to say mine were not salvageable, and looked worse. I've had heavy spore loaded caps look the same when dehydrated. You're probably good.


Yeah, this was with the dehydrator setting on my air fryer. Thats a damn shame though - hopefully it was a small harvest.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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