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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29063923 -

All this talk about fresh shrooms... And it's Xmas holidays goddamnit... Tomorrow the kids are going to their mom's place for a week... :crazy2::mushroom2::crazy2:

I greatly prefer fresh too. More intricate visuals. Faster come-up. I sincerely hope that next year I'll be growing woodlovers year round, just for the fresh shrooms. I mean, ovoids, IWL, cyans, azures, it must be possible


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00] * 1
    #29063998 -

QuantumZ00 said:
I mean if I cared about legality I'd probably have taken a lot different roads in my life including not growing shrooms lol

:snoopyes:

Argh maybe just one weed plant then... I already have a grow tent...



Fully understood 🤣 but nobody says “damn it smells like magic mushrooms around here!?!?” 🫣

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Re: Ask Quick Questions, Get Quick Answers [Re: PancyanterA]
    #29064013 -




¿¿Why caps broke?? 23°C with 97%HR...

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Re: Ask Quick Questions, Get Quick Answers [Re: Sinopense]
    #29064025 -

What are we actually looking at here?
Is it a grow kit, if so, did you follow the instructions?

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle]
    #29064028 -

It is not a grow kit.

It’s grain spawn with a casing layer of vermiculite. I placed it on perlite because I always get side fruiting, and this way I achieve better pin growth.

I’ve grown them a few times before, but this is the first time the caps have broken.


Edited by Sinopense (12/22/24 09:04 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29064042 -

Churpee Choozle said:
As has been said before, the grains probably haven't had enough time to get properly sterilized. Wouldn't throw in my most valuable stuff...



Is pressure sterilization able to be achieved in stages?

Say 90 minutes required could be completed in 60 and then 30 later?



LewDoja said:
When you do lemon Tek do you throw away what's left over in the filter when you squeeze the juice out afterwards or do you eat that as well?



I have never had nausea from mushrooms. It's been about pushing the intensity and shortening the time. I just mix with the liquid and down the entire lot.

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Re: Ask Quick Questions, Get Quick Answers [Re: Sinopense] * 1
    #29064043 -

Sinopense said:
It is not a grow kit.

It’s grain spawn with a casing layer of vermiculite. I placed it on perlite because I always get side fruiting, and this way I achieve better pin growth.

I’ve grown them a few times before, but this is the first time the caps have broken.






Well...
I recommend reading The Hitchhiker's Guide to the Shroomery

If you've decided to case grain spawn with verm you've not followed any viable tek that I know of.

Edited by MycCycle (12/22/24 09:26 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mustache Spinner]
    #29064046 -

Mustache Spinner said:
Churpee Choozle said:
As has been said before, the grains probably haven't had enough time to get properly sterilized. Wouldn't throw in my most valuable stuff...



Is pressure sterilization able to be achieved in stages?

Say 90 minutes required could be completed in 60 and then 30 later?






No.

It's the total continuous uninterrupted exposure to temperature that kills microorganisms.
If you've done 60 minutes, put it back for 90.

However, 60 minutes may be efficient depending on grain, jar size and temps used.

Edited by MycCycle (12/22/24 09:30 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mustache Spinner] * 1
    #29064050 -

You can PC them again for at least 90 minutes, let cool and use. It isn't a cumulative thing. Plus, (speaking from my experience, not saying you're going to), it seems cool to just wing it and see what happens, until you're super excited over your nice myc and your jars turn to shit.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 1
    #29064061 -

Churpee Choozle said:
You can PC them again for at least 90 minutes, let cool and use. It isn't a cumulative thing. Plus, (speaking from my experience, not saying you're going to), it seems cool to just wing it and see what happens, until you're super excited over your nice myc and your jars turn to shit.



Okay, good to know. I've done agar 2 or 3 times now and pretty happy with the outcome. Have a few plates of clean myc on only the second transfer. So, there are more plates than I have anything to do with at the moment. Going to use the best plate for my first attempt at LC which is why I had the PC out to begin with. Had some brown rice, but want to use oats when done with the "experimental" "can I do this" stage.

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle]
    #29064163 -

Churpee Choozle said:
All this talk about fresh shrooms... And it's Xmas holidays goddamnit... Tomorrow the kids are going to their mom's place for a week... :crazy2::mushroom2::crazy2:

I greatly prefer fresh too. More intricate visuals. Faster come-up. I sincerely hope that next year I'll be growing woodlovers year round, just for the fresh shrooms. I mean, ovoids, IWL, cyans, azures, it must be possible



From the description you've given before of your location, those should do great.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29064223 -

The cyans and azures do great (even though I don't eat azures anymore, still grow them). I used to do a run of galindoi in spring time, and maybe some cubes, for the fresh shrooms. Really hope the ovoids and IWL will take their place...

Oh and the cinctulus of course, forgot I was running those again...

Oh and the Gyms of course, because I had enough projects and shit...

Did I mention I just transferred subaeruginosa? :eek:


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 1
    #29064252 -

Disclaimer: Posted this somewhere else but after some thought I didn't feel it was appropriate there. Removed the previous one and decided to ask here.

Was thinking about changing up my agar recipe for a project and wanted some insight before I take the plunge. I don't have the mental patience to deal with percents and find straight numbers easier for me to work with so forgive me.

Long story short I got my hands on some spore prints(2 +1 unlabeled/forgotten that was cultivated, more than likely a 20 year old PE) and syringes(pan, mex, and azur that I don't plan on touching atm) that are between 10-20 years old. The plan is to hopefully get some mycelium to catalogue away in safer storage, IE dh2o cryo vials for longer term storage. I'm looking for more rhizo growth and just wanted some insight from much more experienced members before I waste my time, plus I have no idea what I'm doing when it comes to such old spores. I don't currently have any extra syringes to hydrate the prints for 24h, perhaps there's an alternative method I haven't been able to find searching.

My new recipe would be as follows:
500ml of dh20
7g lme
8g living jin agar
0.5g of nutritional yeast(boiled for 5 minutes then strained in 100/500ml of water)

Should I even bother with yeast and stick with 10g of lme?

Thank you in advance for your time and patience, cheers!


--------------------
15 year lurker

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile]
    #29064257 -






P. Natalensis


How my Jar looking too you? What you would do when it's ready in the next Day's?

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile] * 1
    #29064261 -

People use percents because it lets us compare recipes without doing math to convert to our own batch size. It doesn't take much to get comfortable with, and at worst adds a few seconds of calculator time (there are useful patterns you may notice that will mostly make it possible to do the calculation in your head, especially when you're using a round water number like 500 ml).

Agar doesn't require yeast. Go ahead and try without. Try adding yeast if you find the growth too light and wispy. Use less nutrient if it's not rhizomorphic enough for your taste.

Edit re. old spores: check out the BRF puck tek. Dip swab in sterile water, swipe print, jam swab into BRF puck.

Edited by Phaethon (12/22/24 01:34 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile]
    #29064315 -

I think that's  a decent recipe...  I've never strained it before. And it doesn't fully dissolve so you might want to add a touch more (1.25g total)
though it's not necessarily going to help with old spore germination.  The pressurized syringe might be worth trying if the regular route doesn't work out.

I've had 10yr+ spores on agar germinate with a hot pour (about the same consistency as this recipe).

Is there a reason you're wanting to change?

Edited by LewDoja (12/22/24 03:15 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29064340 -

If I was to store spores in a sealed medical vial suspended in water, how long would they likely be good for?  And should they be kept at room temp or in the fridge?


--------------------
“Order is needed by the ignorant but it takes a genius to master chaos.”
― Einstein


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29064352 -

LewDoja said:
I think that's  a decent recipe...  I've never strained it before. And it doesn't fully dissolve so you might want to add a touch more (1.25g total)
though it's not necessarily going to help with old spore germination.  The pressurized syringe might be worth trying if the regular route doesn't work out.

I've had 10yr+ spores on agar germinate with a hot pour (about the same consistency as this recipe).

Is there a reason you're wanting to change?



So, based on what Meanwhile said, 14% of LME sounds good? 14% sounds like very much, but I just fuck around really. And I haven't revived anything older than a year yet. But I'm interested in if so, what are the benefits of higher percentages than I do now.

I also use 4% agar by the way. 8gr = 16%. Sounds like way too much. But I can be wrong there as well.

This is what Meanwhile mentioned:
Meanwhile said:
(...)
500ml of dh20
7g lme
8g living jin agar
0.5g of nutritional yeast(boiled for 5 minutes then strained in 100/500ml of water)
(...)




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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29064371 -

KauaiOrca said:
If I was to store spores in a sealed medical vial suspended in water, how long would they likely be good for?  And should they be kept at room temp or in the fridge?



https://www.shroomery.org/forums/showflat.php/Number/27778467/fpart/1/vc/1
It's an ongoing experiment but the read is worth it. It's how I've been cataloging everything I have. It's also great because when I transfer I also make a 'save point', IE after I transfer to a new dish I also throw a couple of small cubes of agar in a vial just in case. No need to store in a fridge.

To reply to everyone else's comments concerning what I ended up doing I went:
1000ml h20
20g agar
16g lme

No yeast or anything like that. I'm wondering if I went too hard on the agar to be honest..


cybork said:
LewDoja said:
I think that's  a decent recipe...  I've never strained it before. And it doesn't fully dissolve so you might want to add a touch more (1.25g total)
though it's not necessarily going to help with old spore germination.  The pressurized syringe might be worth trying if the regular route doesn't work out.

I've had 10yr+ spores on agar germinate with a hot pour (about the same consistency as this recipe).

Is there a reason you're wanting to change?



So, based on what Meanwhile said, 14% of LME sounds good? 14% sounds like very much, but I just fuck around really. And I haven't revived anything older than a year yet. But I'm interested in if so, what are the benefits of higher percentages than I do now.

I also use 4% agar by the way. 8gr = 16%. Sounds like way too much. But I can be wrong there as well.

This is what Meanwhile mentioned:
Meanwhile said:
(...)
500ml of dh20
7g lme
8g living jin agar
0.5g of nutritional yeast(boiled for 5 minutes then strained in 100/500ml of water)
(...)






It'd be 1.4% no?


--------------------
15 year lurker

Edited by Meanwhile (12/22/24 04:04 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Meanwhile]
    #29064380 -

Meanwhile said:
KauaiOrca said:
If I was to store spores in a sealed medical vial suspended in water, how long would they likely be good for?  And should they be kept at room temp or in the fridge?



https://www.shroomery.org/forums/showflat.php/Number/27778467/fpart/1/vc/1
It's an ongoing experiment but the read is worth it. It's how I've been cataloging everything I have. It's also great because when I transfer I also make a 'save point', IE after I transfer to a new dish I also throw a couple of small cubes of agar in a vial just in case. No need to store in a fridge.

To reply to everyone else's comments concerning what I ended up doing I went:
1000ml h20
20g agar
16g lme

No yeast or anything like that. I'm wondering if I went too hard on the agar to be honest..


cybork said:
LewDoja said:
I think that's  a decent recipe...  I've never strained it before. And it doesn't fully dissolve so you might want to add a touch more (1.25g total)
though it's not necessarily going to help with old spore germination.  The pressurized syringe might be worth trying if the regular route doesn't work out.

I've had 10yr+ spores on agar germinate with a hot pour (about the same consistency as this recipe).

Is there a reason you're wanting to change?



So, based on what Meanwhile said, 14% of LME sounds good? 14% sounds like very much, but I just fuck around really. And I haven't revived anything older than a year yet. But I'm interested in if so, what are the benefits of higher percentages than I do now.

I also use 4% agar by the way. 8gr = 16%. Sounds like way too much. But I can be wrong there as well.

This is what Meanwhile mentioned:
Meanwhile said:
(...)
500ml of dh20
7g lme
8g living jin agar
0.5g of nutritional yeast(boiled for 5 minutes then strained in 100/500ml of water)
(...)






It'd be 1.4% no?



Ah yes. Indeed. Pff. And then actually 8g of agar sounds a bit low to me :smile:.

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