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tree frog
sapphic love muffin

Registered: 03/31/14
Posts: 6,997

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#29055259 - 12/13/24 09:18 AM (1 year, 10 months ago)

Assuming he's referring to Pans, which lack the genes for rhizomorphic growth, they run very slow on full nutrient agar. They're also often slow on grains.

--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/13/24 09:24 AM)

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mushboy
modboy

Registered: 04/24/05
Posts: 40,843
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tree frog]

2
#29055264 - 12/13/24 09:26 AM (1 year, 10 months ago)

nah im talking more about rhizo vs tom like why does it matter to him. are pans different?

if they lack the genes why bother? and if hes upping the nutes doesnt that defeat the purpose :confused:

:confused:

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:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,628
Loc: USA
Last seen: 7 hours, 32 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tree frog]

3
#29055271 - 12/13/24 09:36 AM (1 year, 10 months ago)

They also stretch out a lot nicer and it's easier to see clean growth.

Panaeolus sp.

Editing because I didn't see your response before. ingeli is section caerulescenta, which means it could produce rhizomorphs. I like seeing the rhizomorphs if it's capable of producing them.

Ive noticed that if I go too low in nutes, rhizos will also go away, as crazy as that sounds

Edit: I'm a dummy.. I keep mixing up igneli with natalensis. ingeli is section cordisporae. It will not go rhizomorphic 🤦‍♂️ .5% she remains

Edited by PhenoDreamers (12/13/24 03:07 PM)

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shadowpeople
None

Registered: 08/17/24
Posts: 234
Loc: Wonderland

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhenoDreamers]
#29056304 - 12/14/24 03:34 AM (1 year, 10 months ago)

My first time doing swab to agar. The plates I brushed the swab against are not doing anything yet. But the one I shoved the swab in is. It doesn't look too great though. Is the black transferable? It has a mycelium looking growth to it. I do not know if it's just spores from the swab or contamination. Plate is contaminated either way. But if the black is good, I can transfer some.

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hellofresh
𓍊𓋼🍄Ⱄⱄ🍄‍🟫

Registered: 09/22/23
Posts: 1,451
Loc: Multiple markets

Re: AGAR ENVY! (Anything and All things agar!)

[Re: shadowpeople]

2
#29056308 - 12/14/24 03:46 AM (1 year, 10 months ago)

shadowpeople said:

My first time doing swab to agar. The plates I brushed the swab against are not doing anything yet. But the one I shoved the swab in is. It doesn't look too great though. Is the black transferable? It has a mycelium looking growth to it. I do not know if it's just spores from the swab or contamination. Plate is contaminated either way. But if the black is good, I can transfer some.

I don't think anything on that plate looks good. I would wait out the other plates and look for white growth. How long has it been?

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🌾💮🫘🥖

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shadowpeople
None

Registered: 08/17/24
Posts: 234
Loc: Wonderland

Re: AGAR ENVY! (Anything and All things agar!)

[Re: hellofresh]
#29056311 - 12/14/24 04:08 AM (1 year, 10 months ago)

I did on the 10th, 4 days. So, the black not normal with a swab I take it. Didn't think so. :frown:

:frown:

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,967
Loc: South Africa
Last seen: 56 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: shadowpeople]
#29056331 - 12/14/24 04:43 AM (1 year, 10 months ago)

Nothing on that plate is usable.

Did you use clean swabs?

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shadowpeople
None

Registered: 08/17/24
Posts: 234
Loc: Wonderland

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#29056338 - 12/14/24 05:02 AM (1 year, 10 months ago)

I couldn't say how clean they are. swab was given to me along with some others I haven't used yet. They said some of them were little old.

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,967
Loc: South Africa
Last seen: 56 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: shadowpeople]
#29056341 - 12/14/24 05:11 AM (1 year, 10 months ago)

Okay I see, I thought you swabbed the fruit.

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tittlez
Mush obsessed

Registered: 11/25/18
Posts: 211
Loc: Europe
Last seen: 10 months, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#29056883 - 12/14/24 04:53 PM (1 year, 10 months ago)

You think the NE (Nepalese) LC test is looking ok or is that mold?

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catnip40
Puppet∴Master

Registered: 03/09/12
Posts: 2,760
Loc: O∴O∴
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tittlez]

9
#29057294 - 12/15/24 03:35 AM (1 year, 10 months ago)

:crankey:

--------------------

^{∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴}

_{∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴}

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Wall.E
Bacteria's Bitch

Registered: 06/05/20
Posts: 3,075
Loc: The Axiom

Re: AGAR ENVY! (Anything and All things agar!)

6
#29057401 - 12/15/24 08:37 AM (1 year, 10 months ago)

Looks kinda like Danny devito as the penguin

--------------------
Mehhh

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sloug
Strangler

Registered: 02/07/22
Posts: 554
Last seen: 9 days, 15 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Wall.E]
#29057453 - 12/15/24 10:24 AM (1 year, 10 months ago)

tittlez said:

You think the NE (Nepalese) LC test is looking ok or is that mold?

nepalese is a cube species yea?? both of those test plates look dirty. the top one has good uniformed shaped growths, which i like and would probably transfer but, where its radiating out from looks kinda gnarly. i cant tell if its actually discolored or im seeing thru the myc to the blueish agar. id probably toss the lower one but im pretty choosey with my plates

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sloug
Strangler

Registered: 02/07/22
Posts: 554
Last seen: 9 days, 15 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sloug]

1
#29057471 - 12/15/24 10:52 AM (1 year, 10 months ago)

oh also..

TEAM BOTH

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ThurmanMurman
Sandwich Artist

Registered: 06/25/24
Posts: 3,630
Last seen: 1 day, 19 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sloug]
#29057562 - 12/15/24 12:42 PM (1 year, 10 months ago)

LC test plate - 12/8

I'm not sure I like the way it doesn't want to consolidate and become one colony. I think I'm going to let it go another 3 or 4 days before calling it.

Truthfully, I'm not confident in my ability to read this. Thoughts or opinions welcome and appreciated.

--------------------

Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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chfbrd
Stranger

Registered: 09/22/23
Posts: 131
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#29057580 - 12/15/24 12:59 PM (1 year, 10 months ago)

Hello,
Just wondering what these white dots might be? think they are pins or are they contamination? If they ar epics, can I put them to LC or should I put them to Coir?

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ThurmanMurman
Sandwich Artist

Registered: 06/25/24
Posts: 3,630
Last seen: 1 day, 19 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: chfbrd]
#29057584 - 12/15/24 01:00 PM (1 year, 10 months ago)

chfbrd said:

Hello,
Just wondering what these white dots might be? think they are pins or are they contamination? If they ar epics, can I put them to LC or should I put them to Coir?

They look like knots to me, but let someone with more experience verify that. 🤘

--------------------

Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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cronicr

Registered: 08/07/11
Posts: 65,510
Loc: Van Isle
Last seen: 1 day, 19 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ThurmanMurman]
#29057602 - 12/15/24 01:13 PM (1 year, 10 months ago)

:whatshesaid:

--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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sloug
Strangler

Registered: 02/07/22
Posts: 554
Last seen: 9 days, 15 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ThurmanMurman]

1
#29057608 - 12/15/24 01:20 PM (1 year, 10 months ago)

ThurmanMurman said:

LC test plate - 12/8

I'm not sure I like the way it doesn't want to consolidate and become one colony. I think I'm going to let it go another 3 or 4 days before calling it.

Truthfully, I'm not confident in my ability to read this. Thoughts or opinions welcome and appreciated.

the area from 12-3 on the first pic looks a little funky but the images are too blurry to see whats going on exactly. its normal to have multiple sections of growth when putting LC to agar. they will more or less just grow into each other. wont really become "one colony", at least visually.

Edited by sloug (12/15/24 03:59 PM)

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chfbrd
Stranger

Registered: 09/22/23
Posts: 131
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sloug]
#29057684 - 12/15/24 02:15 PM (1 year, 10 months ago)

If they are knots....should I put the plate to coir?

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