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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet]
    #29030095 -

Thanks homies


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29030184 -

I have had trouble colonizing slants in the past. My agar dishes do ok. Some slants do ok, but others just never colonize. I usually squirt a few cc of liquid culture into a slant, and cap it, and let it sit. The LC is good. Sometimes, i also drop wedges, and colonization stops half way. Is this because of lack of gas exchange? How do you position the cap during colonization?


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Re: Ask Quick Questions, Get Quick Answers [Re: space.madness]
    #29030227 -

I can't buy spore prints in my country. Customs don't allow importing them.

I'm planning to have a trip to EU (Austria, Germany, Netherlands). I'd like to know if I can buy spore prints there in person from a shop. If so, can you let me know some good places?

And second question is, does spores contain any metal? I saw them usually on tin foils. I'm asking because I don't want to get question about it in the airport.

For example, seems like this can be found in Amsterdam. Does it contain any metal?


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Re: Ask Quick Questions, Get Quick Answers [Re: space.madness] * 1
    #29030238 -

space.madness said:
I have had trouble colonizing slants in the past. My agar dishes do ok. Some slants do ok, but others just never colonize. I usually squirt a few cc of liquid culture into a slant, and cap it, and let it sit. The LC is good. Sometimes, i also drop wedges, and colonization stops half way. Is this because of lack of gas exchange? How do you position the cap during colonization?



If you fully tightened the cap, it's probably stalling due to lack of gas exchange. If you back the cap off a quarter turn or so and wrap it with a bit of parafilm or cling wrap, it should keep growing. (Unfortunately it will also pin if you forget about it...)

It's a little weird that it works fine when you use LC though. :shrug:

I haven't made slants in a while. After I lost quite a few of them to a fridge freezing event (I have had terrible luck with this), I started using the cryovial tek. It's so much easier to prepare and takes up so little space (none in the fridge).

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Re: Ask Quick Questions, Get Quick Answers [Re: tripground]
    #29030254 -

tripground said:
I can't buy spore prints in my country. Customs don't allow importing them.

I'm planning to have a trip to EU (Austria, Germany, Netherlands). I'd like to know if I can buy spore prints there in person from a shop. If so, can you let me know some good places?

And second question is, does spores contain any metal? I saw them usually on tin foils. I'm asking because I don't want to get question about it in the airport.

For example, seems like this can be found in Amsterdam. Does it contain any metal?





Hey there. As much as I’m sure people would like to help you it is actually against the rules to source or discuss sourcing materials, especially when you may be breaking laws.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29030263 -

Thanks. Exactly what I needed to know


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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29030270 -

Thank you!

It's legal to use spores for microscopy in my country. So, there is actually no issue about legality of having a spore. But I understood you, that's okay.

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Re: Ask Quick Questions, Get Quick Answers [Re: tripground]
    #29030272 -

If it's legal to use spores then it's legal to mail them.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29030274 -

Yes, it's correct. Mailing is not illegal, but it's totally up to the officer at the customs. Most of them, doesn't allow them to be imported because they don't believe that it'll be used for microscopy. I mean if you're lucky, then they will allow it to be imported. But most of time, you only lose your money.

So, buying it in person, and taking it to home with me is the best way to go. Because in this situation, they cannot send it back, because it's legal to have it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 1
    #29030276 -

Mr Piggy said:
Fiet said:





How to avoid this Agar pices are hanging on the Glas I can't shake it off

I do a transfer from Agar today the Strain i use are P. Natalensis ore so i getting this from the Marltplace :smile:




Before taking the lid off to inoculate tip your jar to the side, then tip it back up slowly so the grain is sitting at a slant instead of level.  After you drop your wedge in, tilt the jar to the side again to cover up the wedge.  Do not shake until 1/3 colonized, skip the initial shake.




Cool Tip thanks

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet] * 2
    #29030291 -

This is one of those iykyk... and is still a pain in the ass at times.... Welcome to the frustration club!:minigun:

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Re: Ask Quick Questions, Get Quick Answers [Re: tripground]
    #29030363 -

tripground said:
Yes, it's correct. Mailing is not illegal, but it's totally up to the officer at the customs. Most of them, doesn't allow them to be imported because they don't believe that it'll be used for microscopy. I mean if you're lucky, then they will allow it to be imported. But most of time, you only lose your money.

So, buying it in person, and taking it to home with me is the best way to go. Because in this situation, they cannot send it back, because it's legal to have it.



Assuming you will use them for microscopic purposes only… if getting them mailed is legal, then I’d just try it a couple of times. They are not going to open every regular letter, I assume, so I’d say the chance that it arrives is much bigger than that is doesn’t arrive.

Edit: I actually meant getting prints from the marketplace as pqueue is referring to. I understand you don’t want to try buying a print a couple of times.

Edited by ambigu (11/20/24 03:59 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: tripground] * 1
    #29030404 -

tripground said:
But most of time, you only lose your money.



Hang out here a little longer and get Marketplace access. I'm sure you could get in on one of the spore print giveaways


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:crankbowcloud:

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29030473 -

LewDoja said:
This is one of those iykyk... and is still a pain in the ass at times.... Welcome to the frustration club!:minigun:



Like when a wedge stick to a lid.

:epicfacepalm:


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29030487 -

Question for the farmers here who might know:

When cubensis naturally grow from manure in a pasture, what % of the colonized patty do they output. 10% of the total mass? more?

Each cow shits 30kg per day.

On that note, RR once sneakily mentioned that the trick is to just put some spore prints in the drinking through, and let nature do the rest. They eat spores all of the time on grass, so its not doing them any harm. Maybe LI would be fine lol.

On that note, do we know for certain that the spores only start colonizing the poop after its out, and the spores with it, or do they actually colonize the digested grass still inside of the cow?


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29030546 -

The manure tek I saw mentioned letting the poo compost for at least 6 months.  I believe the poo is the moisture source and the spores colonize the grains in it?

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi]
    #29030553 -

Nah, remember that they eat grass in the wild. According to chatgpt, they only colonize once the poop is out, but the spores are already present, which is what I've learned elsewhere, although I think Paul Stamets says they start colonizing inside of the cow and its very gentle stomachs.

Cows shit 30 kilos per day, the dry content of that is 25% at best, so 7.5kg.

The bio-availability for cubensis on cow poop is apparently 10% of the dry mass, therefore in ideal situations that is 750g wet, 75g dry. Per day.

Six cows could be 1 pound dried per day assuming ideal conditions once its the first flush has happened.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29030556 -

They started to grow in the jar, should i just take it out and put it in the SGFC without dunk and roll 🤔🤔🤔???

Edited by killreflection (11/20/24 01:07 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: killreflection]
    #29030560 -

Potency question:
Basically how do you test potency?  Consuming obviously. But like what measurements should I be looking for to gauge?  Idk how to make it make sense.

lol!

I took 5.5 g GT last night that I grew from all in one bag and LC. very light and short trip.  Still learning


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Agar stuff

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Re: Ask Quick Questions, Get Quick Answers [Re: MycoKyle]
    #29030564 -

MycoKyle said:
Potency question:
Basically how do you test potency?  Consuming obviously. But like what measurements should I be looking for to gauge?  Idk how to make it make sense.

lol!

I took 5.5 g GT last night that I grew from all in one bag and LC. very light and short trip.  Still learning



You can get testing kits but they're not 100% accurate. You have to sacrifice some material to get the test result so it's kind of like testing a hand grenade by pulling the pin.

Ssris and other medication can lower the potency and genetics also plays a factor. I had a betch of cambodians where you had to eat practically 10 G to get a half decent trip. It happens sometimes


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (11/20/24 01:34 PM)

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