ansr said:

Phaethon said:

ansr said:

Phaethon said:

Not mold. Congrats!

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Awesome

I'm guessing there's a lot of phenos in these since it's the first transfer from spores? I will make a bunch more transfers to individual plates

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Really depends on the spores - many varieties are very stable, so you are not likely to get huge variation even from spores. The closer to wild or a cross between varieties, the more likely you will get a lot of variation.

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No I didn't mean for variation, I meant just to make it closer to monocultures so there's less competition.

btw, are you 100% it's cubensis mycelium and not some mold? I'm still worried because that print only made mold before. On agar and on PF cakes and pucks, green mold, and on the other plate I transferred four pieces to, from one of those two plates, three of them quickly got green mold, almost like the mold woke up after transferring because the T0 plates still don't have any green mold? (Used a flamed to red glowing scalpel, can't imagine it was contaminated?) And whatever it is on those two T0 plates, it's growing inside the agar, not on top of it.

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Meanwhile said:

Long story short I kinda suffocated these guys for 2 weeks having the lid latched and they've stalled. Unlatched later on but it's day 30 now. Is there anyway to 'wake up' or shock them into producing or just start over?

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ThurmanMurman said:

Idk if there's an answer to this, but which grain is easiest to determine proper hydration? Idec if it's more work to get it there, I just need to get the hell away from wbs.

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ReverendMyc said:

Another vote for wheat. I haven't run rye yet.

It is easy to use a scalpel to cut open the wheat to see exactly how hydrated it is.

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RockinRobot said:

Meanwhile said:

Long story short I kinda suffocated these guys for 2 weeks having the lid latched and they've stalled. Unlatched later on but it's day 30 now. Is there anyway to 'wake up' or shock them into producing or just start over?

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Just Let the ride.

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mist3rfortunate said:

If I had PC'd them they could last awhile though, right?

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thirdeyewild said:

mist3rfortunate said:

If I had PC'd them they could last awhile though, right?

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Yes

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9months is my 'expatriation date'. also depends on the use.

i have knocked up over year old grain jars with a heafty lc dose. they grew shrooms

certainly no standard practice or anything. freshiest is bestiest

Phaethon said:

Let me post your original pic again to get some other eyes on it:

ansr said:

8 days since transfer from one of those plates. It's very thin and whispy but this is not mold right? Or can mold grow like this too?

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To me that all looks clean. And I think if you had a plate that never had sporulating mold (even after taking wedges from it), but its transfer plates did, you most likely contaminated them while making the transfers. There's a lot more to sterile technique than whether the blade was flamed. In any case, I would be shocked if any of the four colonies in that picture had mold - they look very typical for cubensis to me. I'm a noob compared to many around here (so hopefully a more experience cultivator can also chime in), but I've worked with many different cultures on agar.

Btw, it's not uncommon for mycelium to grow through agar. We talk about two-dimensional growth, but what we really mean is two relatively unrestricted dimensions. The third is restricted "up" by there being no media to colonize and "down" by the media having only a few millimeters of depth. It will still grow into the media to extract the available nutrients, especially as more time passes.

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I did it in a SAB wiped with 70% iso, iso'd gloves, same as I always do but never had green mold on a plate before using spores from this print.

I placed the pieces on the new plate by cutting into the agar so they didn't stick to the scalpel and on three of the four transfers (on another plate, not this one) the mold started from that cut in the agar as if I dragged out the mold from the pieces that had it growing inside. Or it somehow got on the scalpel but I don't see how that happened unless the air in my place is really bad.

It's still very thin and whispy and see through, not thick white, more than how it looks in the picture.
Some other plates from this print are even thinner than this. Almost can't see the mycelium even though it covers the whole plate?

What causes it to grow into the agar instead of growing on top like this?

Fiet said:

Contamination
??

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