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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] * 1
    #29011273 -

ansr said:
Since regular grown mushroom fruits aren't sterile, are all more or less contaminated? If you clone a mushroom using a piece from the outside of it, will it show contamination?


Gotta get all up in dem guts, amigo. Split it open like string cheese and take a bit. Just keep your dick beaters off the flesh so you dont contaminate it.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29011295 -

ThurmanMurman said:
ansr said:
Since regular grown mushroom fruits aren't sterile, are all more or less contaminated? If you clone a mushroom using a piece from the outside of it, will it show contamination?


Gotta get all up in dem guts, amigo. Split it open like string cheese and take a bit. Just keep your dick beaters off the flesh so you dont contaminate it.


I haven't tried it, I'm just asking for knowledge.

Since it is so important to use tissue from inside, does it mean the outside contaminated? How do you know when a fruit is too contaminated to be safe to consume then?

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #29011306 -

It’s because mold spores and bacteria are living on the outside of the fruit. It doesn’t mean that the fruit itself is contaminated, but it will eventually rot from other molds and bacteria.


--------------------
Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29011324 -

How should I transfer mycelium to substrate monotube?

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Re: Ask Quick Questions, Get Quick Answers [Re: Wingnut 13]
    #29011348 -

A bit vague?


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29011363 -

So, in bulk casing grows in a monotub....
I have experienced in a 3rd flush, odd growing patterns in my stems, split stems, starry seams, and a few side shoots. also the appearance of the caps on the 3rd on seperate fruits has show signs of wavy caps.

This is a popular landrace, would this be indicative of a pheno split, or would this be something more akin to a bacterial or biological nuance or a weakening of the genetics or possible dehydration? the cake has been rehydrate for each subsequent flush, also fae and rh has remained stable at fruiting condtions.

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] * 1
    #29011448 -

[
ansr said:
ansr said:
Trying the dead GT print again, now on water agar. Left pic drops of sterilized water that I dropped the specs of spores into, made a little crater in the middle for the water to sit in, read somewhere to try that, right pic dropped specs of spores and spread out some of it with an inoculation loop.




So these two now. Holding it up to the light because it's so thin it's hard to take pictures of.

Mold or cubensis? Or something else? It's not uniform, it's thin and it looks like it's growing into the agar?




8 days since transfer from one of those plates. It's very thin and whispy but this is not mold right? Or can mold grow like this too?


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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] * 2
    #29011461 -

Not mold. Congrats! :smile:


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29011477 -

There is white stuff on cap of my PE, what is it? Safe to continue to grow them or contam?

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Re: Ask Quick Questions, Get Quick Answers [Re: puppersbrew]
    #29011481 -

There is white stuff on cap of my PE, what is it? Safe to continue to grow them or contam? Forgot to add photos to last post



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Re: Ask Quick Questions, Get Quick Answers [Re: puppersbrew]
    #29011492 -

Phaethon said:
Not mold. Congrats! :smile:


Awesome :grin:

I'm guessing there's a lot of phenos in these since it's the first transfer from spores? I will make a bunch more transfers to individual plates

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Re: Ask Quick Questions, Get Quick Answers [Re: puppersbrew]
    #29011493 -

Yes they are safe it's mycelium


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #29011640 -

ansr said:
Phaethon said:
Not mold. Congrats! :smile:


Awesome :grin:

I'm guessing there's a lot of phenos in these since it's the first transfer from spores? I will make a bunch more transfers to individual plates


Really depends on the spores - many varieties are very stable, so you are not likely to get huge variation even from spores. The closer to wild or a cross between varieties, the more likely you will get a lot of variation.


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29011658 -

Phaethon said:
ansr said:
Phaethon said:
Not mold. Congrats! :smile:


Awesome :grin:

I'm guessing there's a lot of phenos in these since it's the first transfer from spores? I will make a bunch more transfers to individual plates


Really depends on the spores - many varieties are very stable, so you are not likely to get huge variation even from spores. The closer to wild or a cross between varieties, the more likely you will get a lot of variation.


No I didn't mean for variation, I meant just to make it closer to monocultures so there's less competition.

btw, are you 100% it's cubensis mycelium and not some mold? I'm still worried because that print only made mold before. On agar and on PF cakes and pucks, green mold, and on the other plate I transferred four pieces to, from one of those two plates, three of them quickly got green mold, almost like the mold woke up after transferring because the T0 plates still don't have any green mold? (Used a flamed to red glowing scalpel, can't imagine it was contaminated?) And whatever it is on those two T0 plates, it's growing inside the agar, not on top of it.

Edited by ansr (11/04/24 05:14 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #29011689 -

is this amount of moisture normal? 


 

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi] * 1
    #29011693 -

Jebi said:
is this amount of moisture normal? 


 


looks quite ok, mycelium seems to dig it!


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29011888 -

ThurmanMurman said:
ansr said:
Since regular grown mushroom fruits aren't sterile, are all more or less contaminated? If you clone a mushroom using a piece from the outside of it, will it show contamination?


Gotta get all up in dem guts, amigo. Split it open like string cheese and take a bit. Just keep your dick beaters off the flesh so you dont contaminate it.


Well, sometimes you can get contams from inside the stipe... I recently found a living larva on an agar plate a day or two after plating the clone tissue! I was not expecting anything like that from this hobby!


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 1
    #29011899 -

KlarnEvil9 said:
ThurmanMurman said:
ansr said:
Since regular grown mushroom fruits aren't sterile, are all more or less contaminated? If you clone a mushroom using a piece from the outside of it, will it show contamination?


Gotta get all up in dem guts, amigo. Split it open like string cheese and take a bit. Just keep your dick beaters off the flesh so you dont contaminate it.


Well, sometimes you can get contams from inside the stipe... I recently found a living larva on an agar plate a day or two after plating the clone tissue! I was not expecting anything like that from this hobby!


I'm far from squeamish, but that might actually give me a jump. Thats crazy shit man!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29011909 -

It made me jump a little. Never expected to see anything moving on a plate!


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29011911 -

Idk if there's an answer to this, but which grain is easiest to determine proper hydration? Idec if it's more work to get it there, I just need to get the hell away from wbs.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Extras: Filter Print Post Top
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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