Fiet said:

Good morning guy's,

4 day's ago i transfer some new Strains to Agar from a Spore Print and from Swaps today i couldn't see any growth how long it takes for the Spores to Germ? Is there maybe a chance the transfer has failed?

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can take from a few days up to weeks. be patient

Fiet said:

Good morning guy's,

4 day's ago i transfer some new Strains to Agar from a Spore Print and from Swaps today i couldn't see any growth how long it takes for the Spores to Germ? Is there maybe a chance the transfer has failed?

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Arkyhog said:

When putting a Plin to a new agar plate, do you bury it or just lay it on the top of the new plate?

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ThurmanMurman said:

If a box contaminates the stumps after the 2nd flush, would that be an indication of anything in particular? I will say that I may have pushed it with the bottom watering- soaked between flushes and then did it again while fruits were probably half grown. Usually 12-18hrs at a time though. May have had excess water on the surface from spilling/draining...

I was just curious if the stumps contaminating was an indication of overdoing it on my part and if I should dial back the watering. Sub was still shrunken a good bit, so figured it might want more water.

Disclaimer: I wont even be pushing for a 3rd after this round. Between getting behind with spawn and doing a bit of a test with my spawn health, I just wanted to see how far I could take it.

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Smellyhobbit said:

ThurmanMurman said:

If a box contaminates the stumps after the 2nd flush, would that be an indication of anything in particular? I will say that I may have pushed it with the bottom watering- soaked between flushes and then did it again while fruits were probably half grown. Usually 12-18hrs at a time though. May have had excess water on the surface from spilling/draining...

I was just curious if the stumps contaminating was an indication of overdoing it on my part and if I should dial back the watering. Sub was still shrunken a good bit, so figured it might want more water.

Disclaimer: I wont even be pushing for a 3rd after this round. Between getting behind with spawn and doing a bit of a test with my spawn health, I just wanted to see how far I could take it.

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Ime stumps shouldn’t contaminate at all. It’s still living tissue, so it should be fine. If your stumps are infected, your sub is probably also infected.

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I appreciate your honesty. They have been shaken 2 or 3 times so far, with the first one probably being too early. The timeline hasn't worried me since it's a PE variety, but what do I know?

I have more of this culture on agar, it has been transferred a few times and is looking better than what went in these jars. These jars are my first attempt at grain prep, so I used what would have been a throwaway plate to knock these up. I've been fully prepared for these to fail, it was meant to be a test of my skills at prepping rye jars.


Let me see if I can get a better pic, that one has weird lighting. So y'all can say "yeah dude, those look fucky". 

Mr Piggy said:

No cube is that slow on grain.  It looks like they are fighting something pretty hard, but doesn’t look like bacteria.  I can’t get good zoom resolution on this phone, but I wouldn’t be surprised to see a lil yeast.

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Well dang. Here are a few more pics of 2 of the jars, hopefully they are better:

mishro said:

Theres definitely mycelium in there. Unfortunately there a ton of mold myc too.

I hsd this happen on 2 jars where the filter wasnt sealed right. Maybe youre lucky and thats the cause. Its a simple fix.


Im sure your next run will go better!

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Thanks for your input, I know the next run will go better 'cause I'm optimistic like that!

I'm using plastic lids with a SHIP and a whatman filter, but I think I took the silicone rings off the lids before use. Could that be the issue? Or should I re-silicone all the filters before the next go?

Also, is there any point in trying to get these to colonize some outdoor type substrate as I dump them? Or just let the squirrels and chipmunks spread the myc around naturally?

Mr Piggy said:

Yeet em into the sun.

I'm not a fan of ships or whatmans.  I like synthetic filter disks and I toss agar directly onto grain.  There's a lot less steps where something can go wrong that way.

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