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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: TheAtlantean]
    #29002326 -

How would you produce 2 dl of spores? If Obama wanted to buy concentrated by volume cubensis spores in large volume, how would it be produced and gathered?

Ya'all can print some spores but can you solve this?


--------------------
Willpower is the one true virtue


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Re: Ask Quick Questions, Get Quick Answers [Re: smalltalk_canceled]
    #29002395 -

smalltalk_canceled said:
How would you produce 2 dl of spores? If Obama wanted to buy concentrated by volume cubensis spores in large volume, how would it be produced and gathered?

Ya'all can print some spores but can you solve this?


Just keep expanding lc’s

And Thurman, I’m pretty sure English is smalltalk’s second language. Think he’s Eastern European if I remember correctly.


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Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: Wall.E] * 1
    #29002406 -

Wall.E said:
smalltalk_canceled said:
How would you produce 2 dl of spores? If Obama wanted to buy concentrated by volume cubensis spores in large volume, how would it be produced and gathered?

Ya'all can print some spores but can you solve this?


Just keep expanding lc’s

And Thurman, I’m pretty sure English is smalltalk’s second language. Think he’s Eastern European if I remember correctly.


Well now I feel like a real cunt. I thought we were on drunken political bullshit or somethin, but likely a case of autocorrect gone bad. My fault, small talk.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29002412 -

Would it be too risky to take a sporulating cap from an invitro fruit and put it to LC? I feel like that'd be a nifty way to score some MS LC, if one could get away with it.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29002419 -

QQ: so the other day I learned the hard way that it's possible for one tub to cross contaminate another tub with its sporulation and then it takes over the whole bin. This happened with my KSSS, which took over two natalensis bins entirely.

What is the science behind this? Is it literally just KSSS mycelium starts overtaking natalensis mycelium completely, or do the spores mix with whatever culture is already in the tub?


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

Edited by QuantumZ00 (10/27/24 07:18 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29002421 -

Hi guys, how long does it usually take for in vitro pins to appear after the plate is fully colonized? If the times are wildly variable your personal experiences would help as well. Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: Radamanto] * 1
    #29002436 -

Radamanto said:
Hi guys, how long does it usually take for in vitro pins to appear after the plate is fully colonized? If the times are wildly variable your personal experiences would help as well. Thanks!


I had 2 plates pin at about 7 weeks, if that helps ya any.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00]
    #29002556 -

ThurmanMurman said:
Wall.E said:
smalltalk_canceled said:
How would you produce 2 dl of spores? If Obama wanted to buy concentrated by volume cubensis spores in large volume, how would it be produced and gathered?

Ya'all can print some spores but can you solve this?


Just keep expanding lc’s

And Thurman, I’m pretty sure English is smalltalk’s second language. Think he’s Eastern European if I remember correctly.


Well now I feel like a real cunt. I thought we were on drunken political bullshit or somethin, but likely a case of autocorrect gone bad. My fault, small talk.


It’s fine, I’m sure he wouldn’t care. I was just letting you know that a surprisingly large amount of people on here speak English as a second or third language. He’s a cool dude worth getting to know.

And if your pin is sporulating on a plate, you could definitely drop that into lc. I’d just be sure to test it prior to putting it to grain.


QuantumZ00 said:
QQ: so the other day I learned the hard way that it's possible for one tub to cross contaminate another tub with its sporulation and then it takes over the whole bin. This happened with my KSSS, which took over two natalensis bins entirely.

What is the science behind this? Is it literally just KSSS mycelium starts overtaking natalensis mycelium completely, or do the spores mix with whatever culture is already in the tub?


In this instance I have no idea. Maybe the kss is colonizing grain? It’s hard for me to imagine nats losing a battle to another species unless it was pans or something fast. You pulled off the unfathomable.


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Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: Wall.E]
    #29002637 -

It was completely unintentional, which makes it more frustrating. I'd like to be able to control such things. I only ever let one single bin sporulate, the others I all harvest before veil fully burst. And only one that sporulated? A KSSS bin. That was sitting next to my bin of recently harvested noodly natalensis. My best canopy to date it was, even!

I was waiting fit a second flush and the bin was taking forever, which shocked me because the entire grow these natalensis genetics took off like a bat out of hell. I was like "what's taking so long?"

The story of how this bin:



Turned into this bin on the second flush:




Will haunt me until I figure out how it works, and if I could turn it to my benefit somehow. Like is this a way I can get a cross type in the long haul, or is that not possible this method?


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00] * 1
    #29002648 -

QuantumZ00 said:
It was completely unintentional, which makes it more frustrating. I'd like to be able to control such things. I only ever let one single bin sporulate, the others I all harvest before veil fully burst. And only one that sporulated? A KSSS bin. That was sitting next to my bin of recently harvested noodly natalensis. My best canopy to date it was, even!

I was waiting fit a second flush and the bin was taking forever, which shocked me because the entire grow these natalensis genetics took off like a bat out of hell. I was like "what's taking so long?"

The story of how this bin:



Turned into this bin on the second flush:




Will haunt me until I figure out how it works, and if I could turn it to my benefit somehow. Like is this a way I can get a cross type in the long haul, or is that not possible this method?


I assume there's no possibility of having accidentally swapped the positioning of the boxes? Is the box that is supposed to be ksss fruiting yet to confirm that it isn't the nat box? I just ask bc I pull my boxes off the shelf and bottom water from the sink, so I could see how someone could accidentally swap things if they moved more than one box at a time...


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00]
    #29002650 -

If it was second flush then there might’ve been more available nutrients for the kss. I know some people have used spent subs as substrate, but it’s not always a guarantee. Crossing at that stage isn’t really the best way to go, that’s better done on a plate or if you want to get really crazy, a microscope and a hood.


--------------------
Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29002663 -

ThurmanMurman said:
I assume there's no possibility of having accidentally swapped the positioning of the boxes? Is the box that is supposed to be ksss fruiting yet to confirm that it isn't the nat box? I just ask bc I pull my boxes off the shelf and bottom water from the sink, so I could see how someone could accidentally swap things if they moved more than one box at a time...


No dude, I make mistakes but I don't make those types of mistakes. And the reason I can guarantee it is because all my KSSS bins except one 12qt are outside composting in the garden. That KSSS bin that sporulated was my last six qt KSSS bin. So all that was left percolating was a bunch of Natalensis bins on a second flush, one 12qt KSSS bin, and a bunch of penis envy jars.

In other words, there was no bins to mix it up with


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

Edited by QuantumZ00 (10/27/24 10:45 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Wall.E]
    #29002668 -

Blows my mind people are able to isolate spores. I'd love to give it a go, but a LFH and suitable microscope,  not to mention the other required equipment, must cost a small fortune.

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #29002674 -

Yeah, it’s not a part of the hobby I’d like to explore


--------------------
Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: Wall.E]
    #29002681 -

If I had infinite money, I'd try it all at least once.


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

Edited by QuantumZ00 (10/27/24 10:59 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #29002682 -

What else would you need besides the lfh and scope? If it's just one more small step of a microscope I dont feel its too bad of an investment.


On a different note I am like hypnotized by your profile picture everytime I see it. If thats your dog its gorgeous!

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Re: Ask Quick Questions, Get Quick Answers [Re: mishro]
    #29002691 -

Yeah it is a pretty nice dog indeed. striking eyes, loyal expression. Prize winner.


One of my dogs Luna:



Old and haggard now, almost 12 years old (in November, she was a birthday dog for me years ago). Been through thick and thin.


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29002787 -

When spawning to bulk, can you put multiple jars of grain spawn each inoculated with different agar plates? One whole plate per each jar. The agar plates are of same genetics that came from the same swab. I was told it's risky for some reason, but idk if it's true or not. But I know Shroomery got me  :thumbup:

Edit: Actually I think all the agar plates are from 2 different swabs that were swabbed from the same mushroom, I can't recall. And I dont know which one is which


--------------------


Edited by Jrey16 (10/28/24 01:15 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29002821 -

That's perfectly fine fam no worries at all


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00] * 3
    #29002825 -

QuantumZ00 said:
Yeah it is a pretty nice dog indeed. striking eyes, loyal expression. Prize winner.


One of my dogs Luna:



Old and haggard now, almost 12 years old (in November, she was a birthday dog for me years ago). Been through thick and thin.




My shadow
He is now my 5 year Olds dog though 😂  just ditched me for my kid


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Mono Tub Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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