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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #28997335 -

But if I leave the fuzzy side up I should be good right? I’ll foil up in the PC. To protect the porous side I suppose


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Agar stuff

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Re: Ask Quick Questions, Get Quick Answers [Re: MycoKyle]
    #28997340 -

I honestly think it works either way. I don't use foil with the sunny side up and don't have issues, but if you keep the sfd dry after taking it out of the pc you are probably fine.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #28997359 -

I use fuzzy side up and no foil and don’t have any issues


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Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: MycoKyle]
    #28997366 -

MycoKyle said:
But if I leave the fuzzy side up I should be good right? I’ll foil up in the PC. To protect the porous side I suppose


I made all of mine fuzzy side up. I haven't had any issues that I'm aware of. I too siliconed around the edge of lid hole AND around the edge of the disc itself. I would assume that the hydrophobic material would be waterproof on both sides, so I figured having the waterproofing glued directly over the hole would provide the most protection.


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Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28997371 -

It appears as though I was over thinking it haha thanks everyone.


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Agar stuff

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Re: Ask Quick Questions, Get Quick Answers [Re: MycoKyle]
    #28997381 -

PC question - does the venting timer start once you can see steam coming out? or would you consider it finished venting at that point


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buy the ticket , take the ride

here and here are good places to start

:bunnypeace:

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Re: Ask Quick Questions, Get Quick Answers [Re: squigglez]
    #28997384 -

When steam starts coming out


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Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: Wall.E]
    #28997395 -

when using a sab, do you guys
1. spray the alchohal and clean out the box then load it
2.  do you clean, load then spray again everything again
3. clean the box, let everything settle then clean the items (jars, agar plates) then put them in and let the air settle again?

do i need to put cling wrap over the holes?

I keep getting contam and i have tried so many different things.

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Re: Ask Quick Questions, Get Quick Answers [Re: squigglez]
    #28997406 -

squigglez said:
PC question - does the venting timer start once you can see steam coming out? or would you consider it finished venting at that point


The venting timer starts when you have a strong hard constant stream of steam coming out.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28997415 -







Is this the right way for make a Spore Print? I clean everything with Iso and place the B+ Cap on the foil drop a bit water on the cap and put the lid on it.

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Re: Ask Quick Questions, Get Quick Answers [Re: gmburns]
    #28997418 -

gmburns said:
when using a sab, do you guys
1. spray the alchohal and clean out the box then load it
2.  do you clean, load then spray again everything again
3. clean the box, let everything settle then clean the items (jars, agar plates) then put them in and let the air settle again?

do i need to put cling wrap over the holes?

I keep getting contam and i have tried so many different things.


I don't mess with anything inside the SAB. I have a cooling rack to keep everything off the floor of the tub and a couple empty dishes to set things on, but don't spray them down. I throw the SAB on my bed and let it chill for maybe an hour and then get to work. Gloved hands get sprayed with iso, scalpel gets sprayed down and flame sterilized outside the tub, and any jars or plates that are going in get sprayed. That's it. I'm very careful of my movement outside the SAB as well as inside it. No spraying toward the arm holes or blasting the torch flame towards the SAB. And moving my hands into and out of the SAB is always slow. Try not to bump it. Leave things open or uncovered for as little time as possible, and try not to move your hands over uncovered plates or jars. Everything happens slowly and methodically. Plan out everything.

I feel like I'm super lazy and clumsy with my SAB work and am convinced every time I pour plates in there that half of them are going to be contaminated, but they never are. :shrug:

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28997448 -

Wall.E said:
When steam starts coming out


Mr Piggy said:
The venting timer starts when you have a strong hard constant stream of steam coming out.


dope thx


--------------------
buy the ticket , take the ride

here and here are good places to start

:bunnypeace:

Edited by squigglez (10/23/24 07:46 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: gmburns]
    #28997454 -

gmburns said:
when using a sab, do you guys
1. spray the alchohal and clean out the box then load it
2.  do you clean, load then spray again everything again
3. clean the box, let everything settle then clean the items (jars, agar plates) then put them in and let the air settle again?

do i need to put cling wrap over the holes?

I keep getting contam and i have tried so many different things.


I was having contam issues and for me wearing a mask, flame sterilizing my scalpel, letting the SAB sit undisturbed for like 10min before, showering, and spraying 70% ISO on hands/arms helped a ton.

Also make sure all windows are closed, all fans are off, and ur arm holes are big enough that you can move without hitting the edge (this was happening to me as well)

Good luck!


--------------------
buy the ticket , take the ride

here and here are good places to start

:bunnypeace:

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #28997475 -

I agree with you about the hydrophobic side being up and allowing less water in. That is exactly what I was getting at.


However maybe if you're using it for an LC jar you would flip it the other way that way in case when you're stirring or shaking or tilting the jar to suck some out. anything.  if the LC inside happens to touch the filter then it has the same effect. Keeps the filter from getting wet lc from getting out.


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Edited by callmehippie (10/23/24 09:22 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28997480 -

For LC that makes sense totally. But for grain jars if it doesn’t really matter then I’ll just keep it rolling


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Agar stuff

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #28997551 -

Fiet said:






Is this the right way for make a Spore Print? I clean everything with Iso and place the B+ Cap on the foil drop a bit water on the cap and put the lid on it.


If you put iso on the foil, your print will be dirty. Foil is sterile off the roll, just tear it off, lay it inner-side-up (or tear off another piece first so it won't really matter) and put the cap down on the foil where you haven't touched it.

I've never had to put water on a cap to get it to print, but if you're having trouble getting it to drop spores, go for it.

https://www.shroomery.org/forums/showflat.php/Number/24461957#24461957


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28997573 -

So I had read a post were they advised someone not to prep coir in the same bucket you use to soak seed/grain as you will end up with nutrients in to and it will contam. Can anyone confirm this and/or explain why washing it wouldn't fix the issue ?


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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #28997583 -

ansr said:
Trying the dead GT print again, now on water agar. Left pic drops of sterilized water that I dropped the specs of spores into, made a little crater in the middle for the water to sit in, read somewhere to try that, right pic dropped specs of spores and spread out some of it with an inoculation loop.




So these two now. Holding it up to the light because it's so thin it's hard to take pictures of.

Mold or cubensis? Or something else? It's not uniform, it's thin and it looks like it's growing into the agar?


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Re: Ask Quick Questions, Get Quick Answers [Re: polyflakes] * 1
    #28997585 -

That is absolutely true you will cross-contaminate.

Don't do it. I believe it's because the tiny little microbes and contaminants that are inside the Oats only die during sterilization so if you're not doing this then simply washing it out won't help. You will end up cross-contaminating and wishing you didn't do so.

I'm not sure if this is a proper explanation why somebody else could chime in but it definitely is a fact that you shouldn't do it.


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28997591 -

Ok that makes sense. Thanks yeah I had got an extra bucket I used for the coir just have 14 quarts coming ready this weekend and trying to avoid buying a 3rd bucket or having to make half now half later as I need to prep like ~2450g of coir


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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