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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #28995192 -

I don't think there's much you can do at that stage except pick whatever is fully grown and the little pins and start a new flush. I'm pretty sure misting it this second won't make those pins have a growth spurt, buy hey I've been surprised before.


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Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

Edited by QuantumZ00 (10/22/24 06:35 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28995198 -

My friend recently had a bunch of jars go and spawn bacteria, inoculated over 3 sessions in a s.a.b. (cleaned before each session) using 3 different plates. Everything else inoculated/transferred during those sessions turned out fine. The only common factor between the contams so far is that the three plates were transfers from the same original plate. The plates were fully colonized and looked totally fine to the untrained eye but I'm wondering if there could've been embedded bacteria that wouldn't have been noticeable to a beginner?
or is it more likely just a coincidence?


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I'm doing research for a character/s in a book that I'm writing. At times I may need to post in-character to realistically develop the character/s and/or plot further.

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Re: Ask Quick Questions, Get Quick Answers [Re: nffk]
    #28995203 -

I mean yes of course there was embedded bacteria somewhere imo. And yes some bacteria can be quite hard to spot early on. I still get confused about identifying it not infrequently.


But also as far as I'm aware, even if you do everything "right", there is always a nonzero percent chance of tubs falling to contam. Happens even to the best. However since all three tubs failed, I have to imagine the origin plate was contaminated without realizing it.


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

Edited by QuantumZ00 (10/22/24 06:45 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: nffk]
    #28995206 -

nffk said:
My friend recently had a bunch of jars go and spawn bacteria, inoculated over 3 sessions in a s.a.b. (cleaned before each session) using 3 different plates. Everything else inoculated/transferred during those sessions turned out fine. The only common factor between the contams so far is that the three plates were transfers from the same original plate. The plates were fully colonized and looked totally fine to the untrained eye but I'm wondering if there could've been embedded bacteria that wouldn't have been noticeable to a beginner?
or is it more likely just a coincidence?



Would need to see the plates in question to answer that. Could just have been bad aseptic technique also

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00]
    #28995222 -

Cheers, with every other bad plate I've seen pretty obvious signs the mycelium wasn't alone in there. It was the lack of red flags that had me questioning if there were bacterial contamination's that just don't interrupt the mycelium on agar. Of course I cant ever rule out the possibility of it all being a coincidence.


--------------------
I'm doing research for a character/s in a book that I'm writing. At times I may need to post in-character to realistically develop the character/s and/or plot further.

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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot]
    #28995225 -

Didn't take pics before using them up unfortunately. Might need to start photographing the plates before going to grain in future :grin:


--------------------
I'm doing research for a character/s in a book that I'm writing. At times I may need to post in-character to realistically develop the character/s and/or plot further.

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Re: Ask Quick Questions, Get Quick Answers [Re: nffk]
    #28995282 -

Hey all, quick question here! I'm 2 for 2 in obtaining a harvest of Golden Teachers, and this time I tried out Blue Meanies. They did great, and I got them in my tub, and they were doing great but have plateaud over the last 4 days. I'll provide a picture. Im just curious on everyone's take. The growth looks a pot different than my Golden Teachers, and I'm not sure if I should just wait longer before introducing some air into it or maybe it's ruined? Any feedback will be greatly appreciated. Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #28995284 -

How pathetic is this? Lol. I mean hey, at least I have mushrooms. But this is by far the worse I've ever had side pins. Guess I'll be getting my clone from the side... lol.





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:greyalien:



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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel] * 2
    #28995291 -

towerofbabel said:
Hey all, quick question here! I'm 2 for 2 in obtaining a harvest of Golden Teachers, and this time I tried out Blue Meanies. They did great, and I got them in my tub, and they were doing great but have plateaud over the last 4 days. I'll provide a picture. Im just curious on everyone's take. The growth looks a pot different than my Golden Teachers, and I'm not sure if I should just wait longer before introducing some air into it or maybe it's ruined? Any feedback will be greatly appreciated. Thanks!


They need air from the start. Don’t restrict FAE on your tubs 👍


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28995304 -

Smellyhobbit said:
towerofbabel said:
Hey all, quick question here! I'm 2 for 2 in obtaining a harvest of Golden Teachers, and this time I tried out Blue Meanies. They did great, and I got them in my tub, and they were doing great but have plateaud over the last 4 days. I'll provide a picture. Im just curious on everyone's take. The growth looks a pot different than my Golden Teachers, and I'm not sure if I should just wait longer before introducing some air into it or maybe it's ruined? Any feedback will be greatly appreciated. Thanks!


They need air from the start. Don’t restrict FAE on your tubs 👍


I had one tub of gt where I restricted all fae (accidentally). It pinned and stayed that way for 2 weeks. Grew just fine once I corrected the mistake


--------------------
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 2
    #28995311 -

Huskies said:
Grab a bit from the 9, 10 and 12 o clock and put them on their own plates.

Since its a t2 (second transfer) I wouldn't quite trust this on grains yet. If you have a spare jar ready then why not. But I wouldn't necessarily use it for g2g. The next agar plates from the sectors above, then sure be confident with it.

So, don't make LC yet, but you can consider it based on your next plates.

If you are confident that the LC you have is good, then  you can inoculate more LC with that. If all of the agar plates are fine then it's probably a safe bet. Just a tiny drop should do.

Overall, my advice is don't grain to grain unless you are VERY certain that you have a clean jar. Starting off with a grain lot that is a tiny bit bacterial sucks.

Are you going to use your current LC to inoculate grain jars? What is your plan for your first batch of grains?


I hope to use my LC to inoculate many jars eventually - I did knock up 4 grain jars with my 1st agar from it. still colonizing. The plates were all made from an LC that I expanded using 2cc of the original culture. I put it to agar to make sure it is clean + find the best growing, most aggressive looking myc to grow out. I feel pretty confident in the cleanliness of the mother jar at this point, and I just want to make sure I'm going at the process correctly. Maybe I'm going overboard trying to preserve genetics since I have nothing left of the white GTs I grew earlier this year (they were really potent and fruited like wild).


ReverendMyc said:
:whathesaid:
Never would I ever ... make an LC or g2g with a culture that I have not grown out yet. Once it is a trusted culture and you are 100% confident in your sterile tek and grain prep, then move ahead with mass production methods.

No sense in mass producing a culture that fruits poorly or has low potency, imho.


Well, I did take my original 2 agar plates from this culture and split them amongst 4 myco qts of rye. So it will be fruiting soon if all stays on track. I really just did that part as a self test - my 1st agar pucks went into my 1st set of grain jars. So far, everything looks clean and appears happy. I guess I'm getting ahead of myself, but the agar work is fun, and I'd like to get my fruiting on a staggered schedule so I always have something fruiting most of the time. I've been at a (fruiting) standstill for a couple of months, just me and my plates.

Much appreciation to everyone here! Thanks to you all for sharing your wisdom!


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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Blueberry Muffin]
    #28995341 -

Coolio. Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28995343 -

Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #28995411 -

Should I pick off and discard the black slimy looking cluster here?



Before anyone says anything I know the cakes aren't cased, I couldn't get the cakes out so I was trying to top fruit but I was getting so many pins inside the jars so I decided to solve the problem with a hammer :wow: and bottom water them until the second flush.

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Re: Ask Quick Questions, Get Quick Answers [Re: Pineapples7]
    #28995414 -

Yeah might as well they won't be growing more, looks like aborts. How slimy?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28995424 -

Huskies said:
Yeah might as well they won't be growing more, looks like aborts. How slimy?


The one on the right isn't too bad, the ones on the left are pretty bad. I wouldn't eat any of them if they were from a shop.

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Re: Ask Quick Questions, Get Quick Answers [Re: Pineapples7]
    #28995430 -

Then toss them and pick earlier if you see aborts going on


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28995435 -

Huskies said:
Then toss them and pick earlier if you see aborts going on


Thanks will do! It was slightly harder to identify as they got a bit bruised stuck in the jars. Next time I will know for sure!

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #28995531 -

towerofbabel said:
Hey all, quick question here! I'm 2 for 2 in obtaining a harvest of Golden Teachers, and this time I tried out Blue Meanies. They did great, and I got them in my tub, and they were doing great but have plateaud over the last 4 days. I'll provide a picture. Im just curious on everyone's take. The growth looks a pot different than my Golden Teachers, and I'm not sure if I should just wait longer before introducing some air into it or maybe it's ruined? Any feedback will be greatly appreciated. Thanks!


Everything looks good right now. I would take out those red plugs and put in the foam filters that come with it. At this point in time Fae is definitely beneficial

The different growth is really nothing to worry about that is extremely common even with the same variety. You never really know what you're going to get unless you're cloning and isolating to a point where you force those attributes.


Ps.  How do you like that Boomer shroomer inflatable tub just curious?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #28995538 -

Fiet said:





Looks like the tiny Pins not realy show up they tiny small but the Big ones looking good. What would you do in my case?


I would Harvest those big ones as soon as they're ready clone one. Give those tiny pins just a couple days if you don't see anything happening with them by then I would pick them and move on to your next flush.imo


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Shop: North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Substrate Mix   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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