Seriously_trippin said:

I'll give the lions mane link a consideration thanks


But I specifically want to learn how to grow Pan cyans I don't know if it's high humidity and fae or not. I've searched it here but can't find a guide. But either way I won't be fanning so if it needs more fae than my closed watertub can give I'll have to modify it or maybe cracking the lid ascew is enough I can't really form a plan without knowing what it requires.

I'm thinking watertub but have no idea if I can do a pf tek or if it can't be a watertub because of poor inherent fae because getting the humidity requires a closed or ascew lid.

I'm just wondering from folks who've grown P cyans how I could do this. I don't have a PC and realize I may need 1 for this but if I could do a pf tek or Ef tek or something that'd be great.


Also if there's a common tek for it I'd appreciate the link

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I found the thread it's pinned I'm just tired. I am curious if I can do it with the pf or ef tek and watertub though. The answer to that isn't there.

hydrohomie said:

Hey all. Got some Nats that are hesitant to fruit. Was wondering if anyone had some advice or some recommended condensed reading as to what I can do to induce fruiting?

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Seriously_trippin said:

Seriously_trippin said:

I'll give the lions mane link a consideration thanks


But I specifically want to learn how to grow Pan cyans I don't know if it's high humidity and fae or not. I've searched it here but can't find a guide. But either way I won't be fanning so if it needs more fae than my closed watertub can give I'll have to modify it or maybe cracking the lid ascew is enough I can't really form a plan without knowing what it requires.

I'm thinking watertub but have no idea if I can do a pf tek or if it can't be a watertub because of poor inherent fae because getting the humidity requires a closed or ascew lid.

I'm just wondering from folks who've grown P cyans how I could do this. I don't have a PC and realize I may need 1 for this but if I could do a pf tek or Ef tek or something that'd be great.


Also if there's a common tek for it I'd appreciate the link

---

I found the thread it's pinned I'm just tired. I am curious if I can do it with the pf or ef tek and watertub though. The answer to that isn't there.

---

MacNCheese said:

Likely will make a thread on this topic as the most recent one I found was over 5 years old, and it'd be cool to get into more detail regarding it; but has anyone here tried adding antibiotic agents like myxin/penicillin to their agar?
I recently had a very notable toque fruit that I cloned. The problem is that my sterile technique during this cloning process was less than stellar, and now I am struggling to save these genetics from bacterial contamination. Having done agar work on bacteria in non-sterile conditions, I was wondering if using the same methods used to prevent the growth of contaminant bacteria could be implemented in mycology, making the clean transfer of healthy mycelium much easier?
TLDR: Can you add antibiotics to your agar to reduce bacterial contamination so it is easier to clean up over transfers?

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Seriously_trippin said:

I am curious if I can do it with the pf or ef tek and watertub though. The answer to that isn't there.

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Exotics Fanaticus cakes- V.L.

Chiefychops said:

First time with agar.  And first question,  trying from a spore print I was kindly gifted.
Followed the 101 how too etc, and gone for pastys no pour. Pc done, and made a basic sab, sterilised all etc, using the little pp takeaway sauce pots with the push on lid.  They've got a Fae hole with tape.
Once I've scrapped a teensy bit of spore in and closed the lid, do I wrap the join with a strip of saran? 
And second Q , where is best to keep it? Temp wise etc.    anything else I need to do? 

Thanks in advance

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Seriously_trippin said:

I'll give the lions mane link a consideration thanks


But I specifically want to learn how to grow Pan cyans I don't know if it's high humidity and fae or not. I've searched it here but can't find a guide. But either way I won't be fanning so if it needs more fae than my closed watertub can give I'll have to modify it or maybe cracking the lid ascew is enough I can't really form a plan without knowing what it requires.

I'm thinking watertub but have no idea if I can do a pf tek or if it can't be a watertub because of poor inherent fae because getting the humidity requires a closed or ascew lid.

I'm just wondering from folks who've grown P cyans how I could do this. I don't have a PC and realize I may need 1 for this but if I could do a pf tek or Ef tek or something that'd be great.


Also if there's a common tek for it I'd appreciate the link

---

Chiefychops said:

First time with agar.  And first question,  trying from a spore print I was kindly gifted.
Followed the 101 how too etc, and gone for pastys no pour. Pc done, and made a basic sab, sterilised all etc, using the little pp takeaway sauce pots with the push on lid.  They've got a Fae hole with tape.
Once I've scrapped a teensy bit of spore in and closed the lid, do I wrap the join with a strip of saran? 
And second Q , where is best to keep it? Temp wise etc.    anything else I need to do? 

Thanks in advance

---

Phaethon said:

Yep, tighten them up and also consider storing them in a freezer Ziploc and a cooler inside the fridge to avoid temperature fluctuations drying out (and/or freezing) your cultures.

Also check out the Cryovial Tek. You don't need to rely on refrigeration to preserve cultures.

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Amethyst_Scepter said:

Phaethon said:

Yep, tighten them up and also consider storing them in a freezer Ziploc and a cooler inside the fridge to avoid temperature fluctuations drying out (and/or freezing) your cultures.

Also check out the Cryovial Tek. You don't need to rely on refrigeration to preserve cultures.

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Thank you, I believe it was you who turned me onto cryovial tek a couple weeks back. I just got my vials so im going to do that as well! Whats the longest you've gone with a cryovial? I really appreciate you turning me onto that! I wanna get a shaving cream can like Jurassic park hahahaha

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Oops, sorry - I just always link that when anyone mentions slants because I find them a lot easier.

I haven't been using them all that long, so my longest stasis time to revival so far is about 6 months. But Mycoplex just posted pictures from a fruiting of a culture that was in stasis for two years: https://www.shroomery.org/forums/showflat.php/Number/28987527#28987527