Jrey16 said:

Can I do a transfer from an agar plate and put it back in the fridge for later use?

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Mustache Spinner said:

My question is: I ended up with instant potato consistency rather than a more liquid (what would cool to gelatin consistency).

Will this be an issue? Thanks!

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venetian-cubes said:

Thanks mate, I guess at this stage I'm wondering if I did anything wrong or if it was just the genetics I started with? Impossible to know for sure I guess?

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LewDoja said:

AspectOfTheCreator said:

Is it obvious when LC is contaminated? Ive never messed with LC before, but I recently poured some in a few freshly sterilized grain jars. It looked super clear and clean and is colonizing the grains well. Im assuming if there was a problem with the LC it would be evident at this point?

I know the official recommendation is to test it but to me it just seems the very act of testing it would open it up to contamination, thus making the test pointless. I got my LC in an agar media bottle

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If you're worried about testing on agar FIRST... you can do it LAST.

Since it's already opened for grain inoculations, after the last one put it to agar that way you'll KNOW for sure if at any point (say half way thru) you accidentally contaminated it .

  Or if you really want to get technical.
agar first-->grain jars(×n)-->agar last.

So if the first plate is clean but the last isn't you'll  know it was your technique.

Plus there's the added bonus you get if you succeeded,
CLEAN AGAR PLATES for next time.

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Quote:

You can even thin slice the potato that the mycelium will not turn his face to the dish. Next time do more time in heat to dissolve it and use a french press to filter out the remaining potato flakes

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venetian-cubes said:

Not sure if this helps

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Mustache Spinner said:

Quote:

You can even thin slice the potato that the mycelium will not turn his face to the dish. Next time do more time in heat to dissolve it and use a french press to filter out the remaining potato flakes

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So, should I remake or reconstitute what I've made or will it work as is this time?

My thinking was that it seems like too much potato flake, it consumed all the water pretty much straight away (rather than it need cooking longer).

My assumption is that if it's got nutrients and isn't in a liquid state and pressure sterilized there won't be any problem but I'd rather just get it straight from the get-go.

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Quote:

Potato infusion can be made by boiling 200 grams (7.1 oz) of sliced (washed but unpeeled) potatoes in ~ 1 litre (0.22 imp gal; 0.26 US gal) distilled water for 30 minutes and then decanting or straining the broth through cheesecloth. Distilled water is added such that the total volume of the suspension is 1 litre (0.22 imp gal; 0.26 US gal). 20 grams (0.71 oz) dextrose and 20 grams (0.71 oz) agar powder is then added and the medium is sterilized by autoclaving at 15 pounds per square inch (100 kPa) for 15 minutes.

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- from the Wikipedia article on Potato dextrose agar

It's what I do to make my PDA plates.

PBJ710 said:

venetian-cubes said:

Not sure if this helps

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It looks pretty healthy to me...that makes me think the genetics are likely the cause.  Be patient and maintain the surface conditions.  Some genetic pairings are just slower than others. 

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mishro said:

If you're worried about testing on agar FIRST... you can do it LAST.

Since it's already opened for grain inoculations, after the last one put it to agar that way you'll KNOW for sure if at any point (say half way thru) you accidentally contaminated it .

  Or if you really want to get technical.
agar first-->grain jars(×n)-->agar last.

So if the first plate is clean but the last isn't you'll  know it was your technique.

Plus there's the added bonus you get if you succeeded,
CLEAN AGAR PLATES for next time.

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Quote:

mushroom said
And after all inoculation and aspirating is done I will test again

Long term Im definitely leaning toward agar being a much better solution. LC just seemed appealing when I was starting

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