Seriously_trippin said:

This could be a dumb question because I've successfully done the pf brf verm tek but I'm going to inoculate a new batch tomorrow is there any parts of the process from sub mixing to still air procedure to SAB inoculation technique that you often forget to do or almost forget?

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PBJ710 said:

callmehippie said:

Letsfly24 said:

I’m not sure about that one but I just got this one and like it very much. Plenty big for an at home guy. So nice to have a fan!!

H14 HEPA Filtration Laminar Flow... https://www.amazon.com/dp/B0D1VZ4ZZR?ref=ppx_pop_mob_ap_share

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That one's not really a laminar flow Hood though right? It's more of an ffu? I was looking at that one and others like it as well but then I got super concerned after reading a lot of the reviews. Especially since there were some from people practicing mycology saying it's absolutely not a flowhood. However getting to actually ask you personally instead of reading the review is much better. So how long have you had it? Long enough to judge whether or not it works sufficiently?

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I love my 4x2 FFU that I got from Myers...it may not be a true 'flowhood', but it produces consistent laminar flow across all of the filter and I can leave petris sitting out in the clean output for hours with no contams.  That little bonzai unit is so tiny that you'll have difficulty working on anything more than A2A or jar inoculations and then only a few at a time.  Bags would be next to impossible with it and jar to jar expansions would be difficult at best due to the small area of clean air to work in front to.

The Official Fan Filter Unit(ffu) thread of power

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PBJ710 said:

That FFU on amazon was linked by LetsFly24, but any similar 99.99 @ .3u filtration FFU should work just as well.  The nicer ones have easily replacable prefilters (you may have to rig something for the one linked below) and the fan will have multiple speed settings to allow you to tune things.

Here's an in stock version from the similar items links at the bottom of that page:

Vabiooth FFU

As with any FFU, thoroughly check the filter for damage or torn pleats when it arrives as that will determine it's usefullness.  If the vendor isn't willing to return/replace damage products stay away from them.

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callmehippie said:

And that one's just the filter right? But I can search for that brand then

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PBJ710 said:

Seriously_trippin said:

Also I have an AIO bag starting to fruit in the place I'm gonna put my jars to colonize should I move that bag for possible cross contam?

Aios are prone to contams pretty much by nature and I need that closet for a clean colonizing space

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It's not an issue if your GE filtration works as its designed to.  AIOs are prone to issues due to the lack of testing that often occurs when using vendor MS/LC directly to grain bags that may have not been prepared correctly, are very old or were damaged in shipping/handling.  When people prepare their own cultures knowing they are clean and properly prepare their own grain most of those issues disappear completely.

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Yeah I've definitely been seeing it as a bit of an experiment because I know how easily they can get contaminated from production failures in virtually any step. My dad wanted to see if I could do it and it seems to be going okay I got like 5 pretty strong health pins so far so wish me luck

But I bought a shroomery sponsored one and I think they put them on the list because it was so professionally done. Field capacity seemed spot on and I'm getting some really good fruits even though I don't think the sub fully colonized :/ hard to explain why I initiated fruiting. Rash judgment.

Anyways glad it won't contaminate my colonizing pf cakes in the same closet that was my main worry. And I suppose if I see something concerning in the bag I can move it to the watertub room or move the colonizing jars to a wooden drawer or something thanks

PBJ710 said:

The only thing I can think of that's not exactly what I would consider a perfect FFU would be the lack of prefilter housing, but you can tape a prefilter over the opening if you're not OCD about things like that.  Otherwise it's good to go.

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PBJ710 said:

The PFTek pucks are a somewhat more resistant to bacteria than agar would be due to the physical structure that PFTek has.  I've always used agar for spores and it made it easy to see what was going on, but if the spores were really dirty I could see the advantage of PFTek pucks.  You can tranfer from PFtek to agar or vice versa just like any tissue sample - it's just another growth medium.

Yeah, mine had a metal slot to drop the prefilter into.  At the time it was a little under $800 delivered for a 4x2 - prices have come down on the Chinese units since I bought it 4 years ago.  I did buy mine from an American distributor that has a really good rep in the myco community as a bit of insurance against issues that fortunately never occurred so that made it a bit more expensive than the cheaper ones shipped directly from China.

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ThurmanMurman said:

Wall.E said:

ThurmanMurman said:

Crosspost from Agar thread - this is BAPE. The myc looks wild, but theres no other indication of contamination. It's cool as hell, but is this normal?

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That just looks like wildly tomentose growth. I wouldn’t worry about it

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Good deal. I've never seen it like that, but I like it!

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Sarnath said:

So I was at the grocery store today, picked up some paprika and curry powder at the spice shelf, and next to them were sugars, sweeteners and different baking additives. And today I learned that they use agar-agar in baking too, as some kind of substitute for gelatin. Is that grocery store stuff suitable for our shroomy interests, or are the additives that make it unsuitable?

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Rufusybin said:

EDIT: by ‘sides’, I mean the top around the perimeter, not the actual side view of the side of the entire layers from top to bottom.

Hey alll, did my first s2b and it’s been almost 72 hours post s2b and I’m experiencing slow/uneven recolonization. The sides all around very quickly recolonized, but very little in the mid section. 
I had very healthy colonized grain and used a 1:2 ratio. I know I padded it down “not so gently” and I did not mist any of the pseudo-casing directly; just misted the top and sides and tubs been closed since. I know it’s early, but the fact that the sides colonized so quick and the middle, not, has me scratching my head.

Is there anything I should do at this point? Would opening her up and giving the casing a slight mist be a bad idea? I really appreciate the feedback. I see so many threads on handling s2b fruiting surface conditions, but virtually no troubleshooting on recolonization phase (perhaps that’s because doing nothing is best, not sure) ☮️🤞

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