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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #28945479 -

ThurmanMurman said:


Crosspost from Agar thread - this is BAPE. The myc looks wild, but theres no other indication of contamination. It's cool as hell, but is this normal?



That just looks like wildly tomentose growth. I wouldn’t worry about it


--------------------
Mehhh



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Re: Ask Quick Questions, Get Quick Answers [Re: Wall.E]
    #28945485 -

Wall.E said:
ThurmanMurman said:


Crosspost from Agar thread - this is BAPE. The myc looks wild, but theres no other indication of contamination. It's cool as hell, but is this normal?



That just looks like wildly tomentose growth. I wouldn’t worry about it




Good deal. I've never seen it like that, but I like it!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28945490 -

Whats BAPE, I know SNAPE, and APE, but not BAPE.

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Re: Ask Quick Questions, Get Quick Answers [Re: Azhiaaziam]
    #28945517 -

Azhiaaziam said:
Whats BAPE, I know SNAPE, and APE, but not BAPE.



Blue APE. At least I think that's what it was. I had it given to me and I haven't grown it out yet, so I guess we'll find out together. Lol


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28945524 -

In CA the only site I found with really cool experimental genetics isn't a shroomery sponsor. Has good reviews but I trust the shroomery sponsors with my life and they haven't steered me wrong so far. So it's just B+ and Tifal wave for me for a while.


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 4
    #28945529 -

GenesisCorrupted said:
Letsfly24 said:
4 feet by 4 feet?!







That's 2 feet by 2 feet.


--------------------
🌾💮🫘🥖

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Re: Ask Quick Questions, Get Quick Answers [Re: Letsfly24]
    #28945536 -

Letsfly24 said:
I’m not sure about that one but I just got this one and like it very much. Plenty big for an at home guy. So nice to have a fan!!

H14 HEPA Filtration Laminar Flow... https://www.amazon.com/dp/B0D1VZ4ZZR?ref=ppx_pop_mob_ap_share




That one's not really a laminar flow Hood though right? It's more of an ffu? I was looking at that one and others like it as well but then I got super concerned after reading a lot of the reviews. Especially since there were some from people practicing mycology saying it's absolutely not a flowhood. However getting to actually ask you personally instead of reading the review is much better. So how long have you had it? Long enough to judge whether or not it works sufficiently?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: hellofresh] * 1
    #28945541 -

hellofresh said:
GenesisCorrupted said:
Letsfly24 said:
4 feet by 4 feet?!







That's 2 feet by 2 feet.



:braindamage:
I’m gonna go to bed soon soon. Yes, it is.

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #28945542 -

ThurmanMurman said:
Azhiaaziam said:
Whats BAPE, I know SNAPE, and APE, but not BAPE.



Blue APE. At least I think that's what it was. I had it given to me and I haven't grown it out yet, so I guess we'll find out together. Lol



Haha awesome, I'm here for it!

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28945559 -

I'm coming up on the last of my what I'm calling My Money Pit inventory. Basically all the kit supplies and stuff I bought before finding this site. What I have are two 1 lb bags of popcorn that were sterilized and shipped to me as well as one 3 lb bag of white milo. And then I've got a small little 4 or 8 oz(not sure) block of brf.

That was sterilized and sent to me. Oh yeah and last. I have one pasteurized  5 lb bag of CVG.. all of these were bought online and shipped to me.

What should I do with them? inoculate them cross all my fingers and toes and hope to hell I get something. Which probably isn't likely except for contamination of course... I'm not naive. Anymore.lol

Or should I toss them all into my pc and let them go for a round?  is that even possible to do with these bags that are already sealed? I am unsure of the process of pressure cooking bags if they're sealed before or after. That's why I ask.

In regards to the CVG should I put it through the bucket again to be safe? Is this possible considering it's already at field capacity? Or essentially I guess I could just use it for casings right?

What should I do with the brf? It's just a bag of sterilized brf I think about 8 oz. No dry verm on top. It would be pretty helpful if I could get some opinions please.

Please put yourself in my shoes when offering up opinions. Meaning you you completely understand you fucked up and already wasted money on it and now its just sitting there. is there any way to  any of it better to use and what's the best way to go about it.
All of this is in regards to cubes btw.


PF TEK AWAITS.  I've got the time and I am putting together 24 jars tomorrow. So all of this money Pit inventory  will be a distant memory soon enough.








That's the little block of brf above.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28945566 -

I think you can safely use the BRF bag with a spore syringe (or LC), just treat it like a PF cake.

The grain, inoculate with LC if you have it. Small enough bags you can probably inoculate with an agar wedge in a still air box; I personally was never comfortable opening bags in a SAB, but I've read of others having success.

CVG you should just be able to use as substrate - in the bag or in a monotub - once you have clean grain spawn. Should not need reprocessing.

IMO sterilizing the grain again is probably not worth the effort. If it's sterile, the bags will keep it that way. It may dry out a bit over time, but a few months won't hurt it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28945612 -

Does using Tyvek, courtesy of local post envelope, negate the need to leave jar lids loose? What I'm asking is if there is enough air flow through filter to prevent pressure building up too much so the jars would explode? I hate the idea of air sucking back into jars through loose lids.

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #28945623 -

Yes

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Re: Ask Quick Questions, Get Quick Answers [Re: Cpt.Planet]
    #28945638 -

Awesome. Thanks for the tips


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 4
    #28945641 -

Yay for my 50th post. Everyone who gave me good advice had good ratings coming their way. :grin::grin::mushroom2::crazy2:


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28945658 -

Would something like this



be okay for squirting LC from a syringe into. So I could then easily dip a inoculation Loop into it and make transfers. Seems like this would make  easy to use just the smallest amount of LC necessary and really stretch out what I got. Would they be good for storage as well?

This one is one of the larger ones it's 30 ml. They also have ones that are 10 mL. And probably some in between.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28945663 -

This could be a dumb question because I've successfully done the pf brf verm tek but I'm going to inoculate a new batch tomorrow is there any parts of the process from sub mixing to still air procedure to SAB inoculation technique that you often forget to do or almost forget?


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

Edited by Seriously_trippin (09/14/24 02:27 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #28945678 -

callmehippie said:
Letsfly24 said:
I’m not sure about that one but I just got this one and like it very much. Plenty big for an at home guy. So nice to have a fan!!

H14 HEPA Filtration Laminar Flow... https://www.amazon.com/dp/B0D1VZ4ZZR?ref=ppx_pop_mob_ap_share




That one's not really a laminar flow Hood though right? It's more of an ffu? I was looking at that one and others like it as well but then I got super concerned after reading a lot of the reviews. Especially since there were some from people practicing mycology saying it's absolutely not a flowhood. However getting to actually ask you personally instead of reading the review is much better. So how long have you had it? Long enough to judge whether or not it works sufficiently?



I love my 4x2 FFU that I got from Myers...it may not be a true 'flowhood', but it produces consistent laminar flow across all of the filter and I can leave petris sitting out in the clean output for hours with no contams.  That little bonzai unit is so tiny that you'll have difficulty working on anything more than A2A or jar inoculations and then only a few at a time.  Bags would be next to impossible with it and jar to jar expansions would be difficult at best due to the small area of clean air to work in front to.

The Official Fan Filter Unit(ffu) thread of power

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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
    #28945679 -

Also I have an AIO bag starting to fruit in the place I'm gonna put my jars to colonize should I move that bag for possible cross contam?

Aios are prone to contams pretty much by nature and I need that closet for a clean colonizing space


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28945684 -

Seriously_trippin said:
Also I have an AIO bag starting to fruit in the place I'm gonna put my jars to colonize should I move that bag for possible cross contam?

Aios are prone to contams pretty much by nature and I need that closet for a clean colonizing space



It's not an issue if your GE filtration works as its designed to.  AIOs are prone to issues due to the lack of testing that often occurs when using vendor MS/LC directly to grain bags that may have not been prepared correctly, are very old or were damaged in shipping/handling.  When people prepare their own cultures knowing they are clean and properly prepare their own grain most of those issues disappear completely.

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Shop: North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Myyco.com APE Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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