Jrey16 said:

I was pouring agar earlier in front of an FFU and the agar bottle ending up solidifying right in the middle of me pouring into petri dishes. Can I still be able to reheat it for later use? If so, which is the best way? I have no microwave by the way. I was thinking of putting it in hot water if tht will work, or re-PCing it very quickly just to get it to melt. I stored it in the fridge for now.
And for the presterilized plastic petri dishes that were left over that were fresh out of the packaging.. are they still considered sterilized? Or will I have to sanitize them before pouring? I put them into ziplock bags in front of an FFU and stored them.

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Just throw the bottle in the pressure cooker again for 15-20 minutes or so. No harm done. Hot water wouldn't be enough - I've tried.

Why did you store the media bottle in your fridge? I genuinely don't see the point. Just put it in a cupboard.

If you didn't open the Petris, they're still sterile inside. Oh and if they weren't sterile anymore, there'd be no way to "sanitise" (I think you meant sterilise) them again.

Put the bottle in your PC and pour the rest of your plates. Have fun!

ThurmanMurman said:

Is there any issues with prepping grain and not using it right away? I prepped 14 jars maybe 2-3 weeks ago and put them in a drawer for later. Unmodded lids on tight...I was trying to get it done when I knew I had the time, couldn't see why it would be an issue if I didnt open them, but I saw someones post stating that they wanted to make sure their culture was good before sterilizing grain, which made me wonder if they didnt already have the grain prepped for a reason. Different strokes for different folks and all, it just made me question what I'm doing.

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GenesisCorrupted said:

No.
If you can fit it in your dishwasher, you shouldn’t have any issues afterwards.
People have scrubbed it out with isopropyl alcohol or just soap and water and reuse them no problem.

The filters are dead though.

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Dad_Shroomer said:

Multiple agar 🧫 plates are pinning!! What do I do? Clone…….. How do I do that?

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thirdeyewild said:

ThurmanMurman said:

Is there any issues with prepping grain and not using it right away? I prepped 14 jars maybe 2-3 weeks ago and put them in a drawer for later. Unmodded lids on tight...I was trying to get it done when I knew I had the time, couldn't see why it would be an issue if I didnt open them, but I saw someones post stating that they wanted to make sure their culture was good before sterilizing grain, which made me wonder if they didnt already have the grain prepped for a reason. Different strokes for different folks and all, it just made me question what I'm doing.

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They'll be fine, especially with unmodded lids. No moisture loss that way.

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Bigdogg said:

Dad_Shroomer said:

Multiple agar 🧫 plates are pinning!! What do I do? Clone…….. How do I do that?

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Take one of those pins and put it on a new plate. Tweezers make it easier. Might want to rip the caps off first so there is no chance you germinate spores in there.

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monorail64 said:

callmehippie said:

Phaethon said:

You don't need to test anything on agar to use PF tek. The whole point is its resistance to bacterial contamination and small size so in the event of a rogue contaminant, you've only lost a little jar.

You can also go from BRF to agar, once you have germination. Just make a short BRF puck without a top layer of dry verm, and inject it with the syringe in a still air box. When it's got nice growth you can cut out with a scalpel, make that transfer to agar.

The point of LC is to inoculate grains or sometimes Muda bottles (all in ones, look up Muda bottle tek). It usually goes faster than agar wedges, so people like it for that, and it's easy to make enough to inoculate a ton of jars with very little effort. Requires care to ensure it's squeaky clean.

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Do you have to take any extra measures when making the brf puck without the top dry layer of verm? Cuz doesn't that dry layer help keep it from getting contaminated from the top? Should I be putting micro four tape or something over the holes if I'm not doing the dry layer?

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I've seen people convincingly argue that the dry verm layer is not needed and that it's enough to put a piece of tin foil on top and just leave the jars well alone. The argument being that any contamination with PF would be due to incomplete sterilisation or would occur during inoculation.

That being said, I'd err on the side of caution and either go with the dry verm layer (why wouldn't you, after all?) or slap some micropore tape over the holes.

That way, if you're just starting out, it's easier to determine what may have gone wrong if you do get contamination.

Edit: If you're working with pucks and are planning on transfering to agar from them, I'd definitely bother with the micropore tape/SFDs. To inoculate pucks, open the jars a smidge inside your SAB as described in Josex' Poke and squirt a tiny bit of spore solution in there.

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callmehippie said:

Has anyone posted a tek of the brf to agar process? I'd like to read into more if possible.

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callmehippie said:

Does anyone know if the flow Hoods from Bonsai are any good?

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Letsfly24 said:

4 feet by 4 feet?!

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