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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #28898303 -

Oh alright thanks for the info. I'll have to rewatch it when I get back home

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28898304 -

Seriously_trippin said:
Yeah the only real goal is to work in there so arms straight out does sound very cumbersome. Raising your seat sounds like a good option. I actually used my dad's shower chair to get some more height. Practice makes perfect.




Speaking of which I got my own 5 alarm crap fire now possibly. I think I'm on Day 7 of colonization and the first 6 days my jars looked like they were straight out of a time-lapse of perfect mycelium growth. They still are looking great but we just had a day where the humidity got up to damn near 100% and it looks like my jars unfortunately caught some of that moisture in the air. They're on a wire rack in a shelf in a closet I only opened once today.


And they look like this:


Should I be worried? Is there any place I can put them to be protected from the humidity?

Before today there was no visible moisture. All of it was at field capacity and growing perfect. Fuck. I also got my dad's GroMagic bag coming in tomorrow. Wondering if I should wait to inoculate now :smh:



Just bumping so it doesn't get lost in the thread.


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28898317 -

Quote:
Seriously_trippin said:
And they look like this:



Should I be worried? Is there any place I can put them to be protected from the humidity?

Before today there was no visible moisture. All of it was at field capacity and growing perfect. Fuck. I also got my dad's GroMagic bag coming in tomorrow. Wondering if I should wait to inoculate now :smh:



Looks normal to me, doubt it's from the humidity in the air; in my experience brf cakes will look a little bit sweaty as they colonize more


--------------------
So anyway, uh...

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Re: Ask Quick Questions, Get Quick Answers [Re: So Anyway] * 1
    #28898320 -

Oh thank god. I can sleep well tonight. Probably just a coincidence. It's was so humid today and I live in a desert I'm not very firmiliar with humidity. If it's natural then I'm still loving my grow and just now am seeing the natural process at work. Truly thanks :hug:


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Polkahontass]
    #28898321 -

Do that, videos can be really helpful sometimes, and you can also practice doing it outside the SAB like I've setup in the collage.

I feel like I should issue a disclaimer, I don't think this technique is the best if you don't have a solid sterile technique and high confidence in your SAB work, my 30 odd jars is a small sample number and I take zero credit for this technique.

I could be incredibly lucky, I mostly tried it out because I had way too many grain jars and plates, I expected some of them to fail, none of them did so I kept doing it, and now I'm spawning jars to ziplocs to stuff wherever I can fit them :rofl2:

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Re: Ask Quick Questions, Get Quick Answers [Re: space.madness]
    #28898338 -

space.madness said:
Coconutz said:
Hey guys, can you stack jars on top of each other when doing a steam bath for PFtek?



Yes, but you will reduce the amount of surface area that absorbs heat from the steam, so it will take longer for each jar to reach temp, reducing the amount of microbe reduction that occurs




Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: Coconutz]
    #28898375 -

Coconutz said:
space.madness said:
Coconutz said:
Hey guys, can you stack jars on top of each other when doing a steam bath for PFtek?



Yes, but you will reduce the amount of surface area that absorbs heat from the steam, so it will take longer for each jar to reach temp, reducing the amount of microbe reduction that occurs




Thanks!



With a PC you can stack jars and still do about 60-90 min? Doing my first homemade sterile jars later today.


--------------------
Hitchhiker's Guide to the Shroomery


“Once in a while you get shown the light, in the strangest of places if you look at it right.”

Edited by UtahGetMeTwo (08/03/24 08:03 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28898463 -

Once a jar is fully colonized is it okay to open it up very quick in front of an FFU to smell it before setting everything up to make sure it's good and doesn't smell bad? Or does that risk contamination?
Because the other week I set everything up to spawn but once it was time to pour the grains into the tub I opened up the grain jar and it smelled very sour. Wasted my time. The jar looked good but it smelled bad. I have jars fully colonized right now so I was just wondering. Don't wanna waste my time setting up again


--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #28898559 -

I have a few stupid questios because of the juggling of various teks I'm starting to confuse myself and give myself anxiety about missing something.

I've finally just got my first ever grain fully colonized in bags and jars, and of dozens of jars and bags only one was outright contaminated so I was pretty happy there.

The question is for my CVG mixture, once I pasteurize it using Bucket Tek and then mix it with my substrate, I have head various perspectives on whether I'm supposed to mist it on top of the casing layer after I'm done evening out the top layer or if the substrate when at proper field capacity requires an additional misting at this point at all. So if the field capacity is good in my CVG mix, do I still need to mist prior to closing the lid? Or is it simply a matter of watching from this point forward to make sure it doesn't get too dry at any point? Also I forgot to 'soak' my colonized grain spawn after opening the jars and bags, but this will only effect the quality of my yield, not whether I yield at all?

I followed bods unmodified monotub Tek where I flipped lid upside down and made no distinction between fruiting and colonization once I spawned to bulk, so I'm guessing this means I need to be a bit more deliberate to ensure the tub doesn't dry out?


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00]
    #28898566 -

If you put everything at field capacity, there’s no reason to spray after you just made it. That would mean that it wasn’t at field capacity so you had to spray it.

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00]
    #28898598 -

The misting of the top layer is done by those that prep the substrate UNDER field capacity....in theory,  gives the mycelium a direction to grow towards and reduces side /bottom pins.

Congratulations on first s2b you're hard work is almost paid off, now the hard part.... learning to LITFA!

Edited by LewDoja (08/03/24 11:44 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #28898613 -

Jrey16 said:
Once a jar is fully colonized is it okay to open it up very quick in front of an FFU to smell it before setting everything up to make sure it's good and doesn't smell bad? Or does that risk contamination?
Because the other week I set everything up to spawn but once it was time to pour the grains into the tub I opened up the grain jar and it smelled very sour. Wasted my time. The jar looked good but it smelled bad. I have jars fully colonized right now so I was just wondering. Don't wanna waste my time setting up again




You  can shake them a head of time, since they need to be shaked any ways to spawn, and if they don't recover its bad. If they start to recover within 24-48hrs send it ...

Also if your jar lid holes are big enough, sometimes after shaking, you'll be able to smell that potent good myc or foul sourness. Not always but worth trying .

But to answer the question if you think it's ready for s2b, it's fine to open, don't need the ffu... just don't wait too long to spawn, cause it'll need FAE not  just GE then. As molds prefer stagnant environments.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #28898618 -

Is it okay to stack pf jars in a PC without adding extra time to the PC cycle?


--------------------
Hitchhiker's Guide to the Shroomery


“Once in a while you get shown the light, in the strangest of places if you look at it right.”

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Re: Ask Quick Questions, Get Quick Answers [Re: UtahGetMeTwo] * 1
    #28898627 -

Since Pf tek doesn't require a PC, and stacking normal grain spawn jars/bags is fine I'm gonna say yea your good . Though I have no experience with it. 60-90 mins with a proper venting  should be adequate with stacked jars.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28898642 -

Phaethon said:
StophJS said:
GenesisCorrupted said:
Stopping you right there.
Why make a post about you not wanting to use the very easy (less than a dollar) method.
:agar:
Buying grain or lc sent through the mail wouldn’t make me feel better.
I want to see a pic of these “fine” lids.






Thought I did a good job with them, but perhaps not. As I said I will use agar next time. But I don't want the same thing to happen, and I suspect it will.



In addition to the earlier recommendations to test LC before using it and avoid the injection ports (you can just open the lid in your SAB and add liquid culture), I am a little suspicious of the almost see-through filter. At least the ones I've used are quite thick, and when placed on a table like that you can't see the hole in the lid.

I think the other things are more likely culprits, but you could run a control with an unmodified lid, left just a bit loose while colonizing. Unmodified has some nice advantages - notably, you can dishwash them, you never need to worry about the filter getting wet, and there is no seal around the filter that can fail - you might find you prefer it anyway.

If the filter is one of those SFD stickers, you might at least put one on the inside of the lid as well.

Edit: another control you should run is leaving a jar uninoculated, but shaken on the same schedule as some inoculated ones. If the uninoculated jar gets contaminated, you have a problem with grain prep or lid/filter efficacy.



Thanks for that food for thought. I think the thing that strikes me about how these jars have gone is just how instantly the oats seem to reject mycelium growth. They end up colonizing but are almost rubbery in the end. Here's a couple of pictures for reference.





There's nothing normal about it even for a minute. Kind of funny but I did send some if these to bulk as an experiment. I got a handful of healthy mushrooms after a month and then the rest began aborting and having slimy deformed caps.

Edited by StophJS (08/03/24 01:20 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS]
    #28898720 -





First try with agar. I’m not sure if I did the clone properly, or if it’s just slower than LC. The one that says “water drop” had soapy water drop in from the top of my SAB (lesson learned, don’t spray the roof) and red dots are popping up in that spot. Should I transfer away from that? I have a lot of reading left to do for what comes after making the plates but I haven’t had the time this week so I’m not really sure when to transfer anything honestly.

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28898759 -

Clone tissue can take a little while to establish. That looks fine to me for two days since plating.

As for the plate contaminated by the water drop, yeah, make a transfer soon and remember not to send that plate to grain :wink:

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28898841 -

Hi all. I'm confused by a comment I've read on this site many times. How come a SHIP is a larger contam vector than lifting a lid and squirting LC on the grains? I can't get my mind around it.

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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28898844 -

The idea would be that the SHIP leaves it a tiny bit vulnerable forever, whereas inoculating in a SAB with proper sterilization leaves it very minimally vulnerable for only a moment.

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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28898845 -

If the jar and its contents are sterile. And you’ve pressure cooked your broth and you’ve pulled it into a sterile needle inside of a still air box.
Or in front of a flow hood.
That will be cleaner than some thing that you’ve shoved fluids through.
If that makes sense.

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Jump to top Pages: < First | | 1987 | 1988 | 1989 | 1990 | 1991 | 1992 | 1993 | 1994 | 1995 | 1996 | 1997 | 1998 | 1999 | 2000 | 2001 | 2002 | 2003 | 2004 | 2005 | 2006 | 2007 | | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Substrate Mix


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