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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28897994 -

Seriously_trippin said:
Tri-Polar said:
No need to do the multiple iso wipes, and alcohol sanitizes, it does not sterilize

Otherwise
:kenthumbup:



Yeah I probably shouldve cleaned it just once because it was dirty.I think it just made me less nervous. Good to know though at least I was sanitizing I honestly didn't realize.

Knowing that, it was probably more important that I shut off the air, closed the windows, washed my hands thoroughly, made slow movements putting my hands through, the water with soap dabbing it in odd places in the SAB etc.

I really thought cleaning was half the battle lol. That's why I framed it as what I did because I don't feel like I can give solid advice yet lol.




Hey, I’m always an advocate for doing whatever will make you warm and fuzziest, so long as it’s not a detriment :smile:

I still spray iso sometimes, I know I shouldn’t, but it makes me happy so I do.

But yeah the most important concept imo to internalize is that it’s a STILL air box, not a CLEAN air box. Physics is the name of the game.

Sounds like you got it down though good shit


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #28898014 -

Hey, I’m always an advocate for doing whatever will make you warm and fuzziest, so long as it’s not a detriment :smile:

I still spray iso sometimes, I know I shouldn’t, but it makes me happy so I do.

But yeah the most important concept imo to internalize is that it’s a STILL air box, not a CLEAN air box. Physics is the name of the game.

Sounds like you got it down though good shit



Why do you say that you shouldn't spray ISO? Ive been spraying it on work surfaces and SAB and now I'm wondering if there's more recent info that I missed.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28898020 -

Well if you spray iso inside your sab and get to close with a flame it's prone to explosion which some see as bad

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28898025 -

I feel like that was just misphrased. There's no problem with spraying iso unless it's in an SAB while you're working in it. I spray, let it settle and then get to work.

Assuming that spraying the iso provided a sterile environment is wrong. It will kill some stuff. It will help knock some stuff out of the air once it settles. Sterilize the environment it won't do tho.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28898028 -

True. I started out with an SAB like that. Didn't go so well. And he is right about all that fancy lookin bs they sell online. Garbage. I used to fall for products like those being sold online. They prey on beginners or people having a hard time.. or impatient people. And don't get me started on those big youtubers who prey on beginners spreading misinformation and selling their bs products... I'm still a beginner myself, I started back in January with still a lot to learn, but in my experience products like that online just made it worse for me and wasted my money.


--------------------


Edited by Jrey16 (08/02/24 08:20 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #28898049 -

boxtrotalpha said:
Well if you spray iso inside your sab and get to close with a flame it's prone to explosion which some see as bad



Yeah, if youre running your flame inside the box or near the arm holes maybe, which isn't something to do. I feel like either of those things would be bad for maintaining still air, even without the risk of explosion. I would hope the average person would know better than to blow up their SAB or at least have read someones warning about it. Man, we really do take common sense for granted sometimes...


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28898056 -

ThurmanMurman said:
boxtrotalpha said:
Well if you spray iso inside your sab and get to close with a flame it's prone to explosion which some see as bad



Yeah, if youre running your flame inside the box or near the arm holes maybe, which isn't something to do. I feel like either of those things would be bad for maintaining still air, even without the risk of explosion. I would hope the average person would know better than to blow up their SAB or at least have read someones warning about it. Man, we really do take common sense for granted sometimes...



GenesisCorrupted said:
New growers using isopropyl alcohol in their SAB.




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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28898061 -

GenesisCorrupted said:
ThurmanMurman said:
boxtrotalpha said:
Well if you spray iso inside your sab and get to close with a flame it's prone to explosion which some see as bad



Yeah, if youre running your flame inside the box or near the arm holes maybe, which isn't something to do. I feel like either of those things would be bad for maintaining still air, even without the risk of explosion. I would hope the average person would know better than to blow up their SAB or at least have read someones warning about it. Man, we really do take common sense for granted sometimes...



GenesisCorrupted said:
New growers using isopropyl alcohol in their SAB.






Do experienced growers not use Iso? Must be another practice that faded away in my time away from all this? I use a cookie rack for a floor that sits in a cupboard when not in use. Somebodys gonna have to pry the iso from my cold dead hands!!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 4
    #28898064 -

I wipe my table and inside of my SAB with iso. Sometimes. Sometimes I just use a damp cloth. The iso matters less than people assume. I don’t wipe down anything else, and a paper towel damp with sink water appears to do the exact same thing.

We’re not trying to kill germs so much as we’re trying to eliminate moving particles


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28898067 -

It still definitely has its place. I just feel like when people are starting out. They get a lot of information and they try to apply all of it.
So there are people that are spraying a bunch of isopropyl alcohol inside of their SAB.
That isn’t really necessary. You just want the air to be still it doesn’t need to be sterile inside.
Then, as has been discussed when people try to flame sterilize something. They blow up their sab.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28898075 -

Smellyhobbit said:
I wipe my table and inside of my SAB with iso. Sometimes. Sometimes I just use a damp cloth. The iso matters less than people assume. I don’t wipe down anything else, and a paper towel damp with sink water appears to do the exact same thing.

We’re not trying to kill germs so much as we’re trying to eliminate moving particles



I understand the point being to use physics to avoid contamination, but what should I be doing about the fact that my raised floor is a cookie rack that sits in the cupboard when not in use? It seems kind of pointless to use a false floor to avoid the contaminates on the floor when the cookie rack is just as dirty, no?
GenesisCorrupted said:
It still definitely has its place. I just feel like when people are starting out. They get a lot of information and they try to apply all of it.
So there are people that are spraying a bunch of isopropyl alcohol inside of their SAB.
That isn’t really necessary. You just want the air to be still it doesn’t need to be sterile inside.
Then, as has been discussed when people try to flame sterilize something. They blow up their sab.




I'm sure that some are overestimating its stopping power, but I feel like the torch shouldn't be in the box or near the arm holes anyway, when still air is the goal. Im not a seasoned vet in this hobby, but theres a ton of heat in my line of work and I would imagine the air turbulence and drafts I've experienced in buildings would be happening in the box, just on a smaller scale. Ive also been exposed to a lot of chemicals too though, so maybe I'm wandering around with one foot nailed to the floor.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 3
    #28898079 -

Sterilize outside the box. Never put your flame inside the box.

I don’t use a false bottom or damp towel. I just go right on the countertop without issue.

A SAB is literal magic. The fact that you can use a flipped over tub with arm holes to do STERILE work is magic. We overthink it a little, imo


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28898093 -

GenesisCorrupted said:
It still definitely has its place. I just feel like when people are starting out. They get a lot of information and they try to apply all of it.
So there are people that are spraying a bunch of isopropyl alcohol inside of their SAB.
That isn’t really necessary. You just want the air to be still it doesn’t need to be sterile inside.
Then, as has been discussed when people try to flame sterilize something. They blow up their sab.



Yeah when I realized this I actually thought it was a pretty beautiful concept. Contamination happens from air mostly so is it good to have a clean vs dirty SAB? Yeah, is it good to have a clean workplace? Yeah but the most important thing is the still air itself and washing your hands.

The box itself isn't touching the open inoculation holes and neither is the counter it's on. It's litterally all about that still air inside. That's also why it doesn't hurt to spray/dab on some soapy water in the SAB to catch anything floating in there.

And yes for the record I cleaned my SAB with iso like 8 hours before inoculation definitely don't right before using a torch.


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28898103 -

I have twice now tried making my own grainspawn with Bods easy AF oat tek. The oats are from a feed store and should be fine. The jars have plastic lids with two holes drilled for a SHIP and disc filter. I believe everything is sealed properly. Both times, all six jars ended up bacterial.

I used LC from one of the recommended sources. I did not test on agar, and I know the people here who do agar will stop me right there and want to say that my LC was contaminated. I think it's more likely there is something in the grain than both times in the LC. I do plan on testing on agar next go around but I believe I'll run into the same problem even if I confirm clean culture.

I'm just not sure how to proceed here. I don't want to overpay for prepared grain spawn. Should I try a different grain? I'm really not understanding how it is ending up that every single jar of these oats is bacterial.

Thanks.

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS] * 3
    #28898106 -

I’m going to stop you right there.

You used untested vendor LC in grain, of course it went bacterial.  There is no β€œtrusted source” besides your own work, period.


--------------------
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„ΈπŸ„·πŸ„ΎπŸ„Ώ


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS] * 1
    #28898107 -

Stopping you right there.
Why make a post about you not wanting to use the very easy (less than a dollar) method.
:agar:
Buying grain or lc sent through the mail wouldn’t make me feel better.
I want to see a pic of these β€œfine” lids.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28898113 -

I have twice now tried making my own grainspawn with Bods easy AF oat tek. The oats are from a feed store and should be fine. The jars have plastic lids with two holes drilled for a SHIP and disc filter. I believe everything is sealed properly. Both times, all six jars ended up bacterial.

I used LC from one of the recommended sources. I did not test on agar, and I know the people here who do agar will stop me right there and want to say that my LC was contaminated. I think it's more likely there is something in the grain than both times in the LC. I do plan on testing on agar next go around but I believe I'll run into the same problem even if I confirm clean culture.

I'm just not sure how to proceed here. I don't want to overpay for prepared grain spawn. Should I try a different grain? I'm really not understanding how it is ending up that every single jar of these oats is bacterial.

Thanks.
Mr Piggy said:
I’m going to stop you right there.

You used untested vendor LC in grain, of course it went bacterial.  There is no β€œtrusted source” besides your own work, period.



"Of course it went bacterial". My friend, that doesn't make sense... With all due respect, I do believe we should be able to float a hypothetical in these forums. It cannot assumed that the bacteria is not from the grainspawn or problems with the jars, and it's perfectly reasonable to discuss those possibilities, and frankly, probabilities.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28898116 -


GenesisCorrupted said:
Stopping you right there.
Why make a post about you not wanting to use the very easy (less than a dollar) method.
:agar:
Buying grain or lc sent through the mail wouldn’t make me feel better.
I want to see a pic of these β€œfine” lids.






Thought I did a good job with them, but perhaps not. As I said I will use agar next time. But I don't want the same thing to happen, and I suspect it will.

Edited by StophJS (08/02/24 09:51 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS] * 2
    #28898117 -

You’re bringing mystery broth through the mail system and then wondering why it is dirty.

It is pretty notoriously well-known that liquid culture needs to be tested before it’s used, regardless of where it came from.

That’s why everyone is going to keep hammering you about agar.

It’s fun and easy.
Then make your own lc.

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS] * 2
    #28898118 -

You asked a question, you got an answer.  If you’re not looking for honesty don’t ask.


--------------------
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„ΈπŸ„·πŸ„ΎπŸ„Ώ


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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