boxtrotalpha said:

I've got a liquid culture syringe I've ordered. Is it really as simple as making a 5% solution of Karo and water, sterilizing it and shooting a couple cc's in it to grow more LC?

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LewDoja said:

Arctic_Fox said:

Jrey16 said:

Once fully colonized, how long can fully colonized agar plates be stored for in 23-25 degrees celcius? (Around 72-77 degrees fahrenheit)

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I also want to know this. I have a stack of plates from last year that have been stored at room temperature for at least 6 months. I wonder whether they're dead.

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If they've remaind contam free I'd say they are probably good until they start to shrink or crack from moisture loss.

Although they might not be the best choice for spore germination due to lack of moisture.  If you have have some that fit a Mason  jar or any other airtight container they last longer.

Edit : ahh fully colonized plates ...


I've got some over a year although the basement rarely gets above 70°f .6months is about when plins start showing on average.

I'd say give it a try and see if they bounce back, my guess is they will.

If you want to save a culture for long-term storage, get some slants or vials either agar or distilled water

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Arctic_Fox said:

LewDoja said:

Arctic_Fox said:

Jrey16 said:

Once fully colonized, how long can fully colonized agar plates be stored for in 23-25 degrees celcius? (Around 72-77 degrees fahrenheit)

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I also want to know this. I have a stack of plates from last year that have been stored at room temperature for at least 6 months. I wonder whether they're dead.

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If they've remaind contam free I'd say they are probably good until they start to shrink or crack from moisture loss.

Although they might not be the best choice for spore germination due to lack of moisture.  If you have have some that fit a Mason  jar or any other airtight container they last longer.

Edit : ahh fully colonized plates ...


I've got some over a year although the basement rarely gets above 70°f .6months is about when plins start showing on average.

I'd say give it a try and see if they bounce back, my guess is they will.

If you want to save a culture for long-term storage, get some slants or vials either agar or distilled water

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They're definitely not clean cultures. The left side of the one in the image is the best looking one I have. I'm just trying to decide if I should bother trying to revive it on a new plate, or if I should toss it. I guess there's nothing to lose by trying.

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I was going to say if they're dry you could always drop chunks in liquid culture to revive and go from there....but if they're dirty....the complete reluctance to actually discard a culture affects many of us. I usually bury them someplace on the "chance"
and poured lots of LC'S into piles of debris and stuff

Kunta Kente said:

Mr Piggy said:

if this is the potency loss study that noobs have been posting lately, it's already been shot full of more holes than swiss cheese.

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Links to the holes?

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It's the thread you posted and then everyone shredded.  15 fruits and no control does not a study make

https://www.shroomery.org/forums/showflat.php/Number/28803328

mushhead said:

Heya guys! I just got a MASSIVE amount of wheat grain free.
I'm talking MASSIVE, about 500lbs of it.
I'm going to use it to attempt to cultivate with but I wanted to see if anyone had experience in using it.
I have tried oats & rye already - opinions, teks, and discussion welcome

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Then you have spawn forever? Wheat works great

it preps its own way, here is one https://www.shroomery.org/forums/showflat.php/Number/23545185

boxtrotalpha said:

I've got a liquid culture syringe I've ordered. Is it really as simple as making a 5% solution of Karo and water, sterilizing it and shooting a couple cc's in it to grow more LC?

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curenado said:

mushhead said:

Heya guys! I just got a MASSIVE amount of wheat grain free.
I'm talking MASSIVE, about 500lbs of it.
I'm going to use it to attempt to cultivate with but I wanted to see if anyone had experience in using it.
I have tried oats & rye already - opinions, teks, and discussion welcome

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Then you have spawn forever? Wheat works great

it preps its own way, here is one https://www.shroomery.org/forums/showflat.php/Number/23545185

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curenado said:

I was going to say if they're dry you could always drop chunks in liquid culture to revive and go from there....but if they're dirty....the complete reluctance to actually discard a culture affects many of us. I usually bury them someplace on the "chance"
and poured lots of LC'S into piles of debris and stuff

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Mr Piggy said:

Just going to say it again, LC is not beginner territory.  People should start with the fundamentals of clean plate work, clean grain spawn, and getting good fruiting conditions.  Without those fundamentals you DO NOT have the skill set to understand what is happening with your grow.

Wanting to "speed things up" does not work.  You cannot speed up this hobby.  This is for the patient and observant.  If you just want a bunch of shrooms quick then spend money on shrooms and not on bogus grow equipment from snake oil vendors.

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boxtrotalpha said:

Mr Piggy said:

Just going to say it again, LC is not beginner territory.  People should start with the fundamentals of clean plate work, clean grain spawn, and getting good fruiting conditions.  Without those fundamentals you DO NOT have the skill set to understand what is happening with your grow.

Wanting to "speed things up" does not work.  You cannot speed up this hobby.  This is for the patient and observant.  If you just want a bunch of shrooms quick then spend money on shrooms and not on bogus grow equipment from snake oil vendors.

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I'm not trying to speed things up just diversifying. As soon as the syringe came in I dropped it into 10 agar plates and 2 grain jars. I was looking at LC as an option only because it seems dummy simple but I can see how clean work would have to be 110% on point so I'll keep that on the back burner until I find out if I botched these plates and grains first

Edit to say: all teks I've followed so far have been from beefs guide or links therein so I'm confident with those and the syringe came from a trusted vendor here so I'm hopeful with my techniques but time will tell

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GenesisCorrupted said:

For God sake, don’t open the bag.
Don’t do anything. It’s a bag that will be contaminated immediately if it is opened. Leave it alone and hope that it actually works.

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Triocd said:

For a grow bag, when pins have formed and are growing, which is most important?

Humidity level of the bag or oxygen content in the substrate/bag? I know both are important and it's best to watch both, but which is the priority?

Opening the bag and adding fresh air more often adds more oxygen but the humidity level will be lower.

Opening the bad less frequently will lead to a higher humidity level but lower oxygen.

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GenesisCorrupted said:

Those bags are so Fugly. There’s always some contamination in them. I don’t feel like opening the bags ever a good idea. Wait until they’re literally fucking pushing out of the top of the bag. But rubber bands around it and then top fruit it.

But those bags are traumatizing I would never open them again unless it’s done.

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