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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Cassette81]
    #28825066 -

the jar is 50/50 but contam seems to be loosing the battle. it's been sat for about 2month


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IMG_20210822_150455

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Re: Ask Quick Questions, Get Quick Answers [Re: Cassette81]
    #28825067 -

Cassette81 said:
what would you do next?


Throw it out and start over.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Cassette81] * 2
    #28825069 -

Cassette81 said:
what would you do next?


Yeet it into the sun


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: Cassette81] * 1
    #28825070 -

Cassette81 said:
the jar is 50/50 but contam seems to be loosing the battle. it's been sat for about 2month


Mold does not lose battles with cube myc


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28825074 -

Or any contam for that matter.  They can be fruited if bacterial, but anything else should be thrown out.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: LikeMyc]
    #28825078 -

LikeMyc said:
Fully colonized doesn't mean a flawless coat of white. Mycelium may not cling to every single little thing in the jar when you have a mixture of different "grains".  Dont worry about metabolites.


That is cream cheese bacteria soup and nothing else.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Edited by Mr Piggy (06/24/24 08:53 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 2
    #28825080 -

obviously I'm a novice but
I'll clean up my act and go again
thanks


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Re: Ask Quick Questions, Get Quick Answers [Re: Cassette81]
    #28825123 -

How do these look to y'all? They are all T3 from an Avery's Albino spore swab. The third one is obviously bacterial as fuck all around the culture but I thought I'd include it for the sake of showing the growth. I am still new to agar and dialing in my sterile procedures. I'm paranoid I'm growing some mold I'm not familiar with but at the same time unsure if it's just some tomentose mycelium? Just seems too wispy.





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Re: Ask Quick Questions, Get Quick Answers [Re: Pennock]
    #28825147 -

Have 2 questions:
I made a small mistake and got tubs that don't have clear lids. Is it really necessary to cut hole in the top and put cling film wrap on it to let light in, or is light for the side of tubs fine?
Also how important is it to use 6000k lights/ sunlight. What effect would using a normal LED bulb have? I'm having a hard time finding consistent info on light, I feel like I'm missing something.

Edited by Coconutz (06/24/24 09:41 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Coconutz] * 1
    #28825155 -

My shoeboxes have opaque white lids, some grey even. No need to mutilate the lids. My lights are probably 5000 K, definitely not 6500 K. Higher color temperature is probably better but it doesn't seem to matter that much, as long as they get some light every day. (I use a timer set to a 12/12 schedule. You can get away with indirect sunlight from a window.)


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28825161 -

This is exactly what I was hoping for, thanks a bunch this definitely saved me some hassle! Appreciate it! :smile:

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Re: Ask Quick Questions, Get Quick Answers [Re: Pennock]
    #28825168 -

Pennock said:
How do these look to y'all? They are all T3 from an Avery's Albino spore swab. The third one is obviously bacterial as fuck all around the culture but I thought I'd include it for the sake of showing the growth. I am still new to agar and dialing in my sterile procedures. I'm paranoid I'm growing some mold I'm not familiar with but at the same time unsure if it's just some tomentose mycelium? Just seems too wispy.







The mycelium looks a little squiggly and disorganized...but I wonder if I'm just seeing the texture of the background through it. Can you get a picture of one against a solid, untextured background?

What happened with #3?


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28825201 -

How's this? I threw them on a sticky note cuz that was in reach but I can probably find a better spot if this is still too tough to make out.

As for #3, couldn't tell you for sure, but I'm sure it involves my lack of experience with agar, as well as my shaky, nervous hands lol


Edited by Pennock (06/29/24 03:52 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Pennock]
    #28825203 -

Pennock said:
How do these look to y'all? They are all T3 from an Avery's Albino spore swab. The third one is obviously bacterial as fuck all around the culture but I thought I'd include it for the sake of showing the growth. I am still new to agar and dialing in my sterile procedures. I'm paranoid I'm growing some mold I'm not familiar with but at the same time unsure if it's just some tomentose mycelium? Just seems too wispy.







How long did they take from transfer to get to where they are?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28825205 -

9 days since the last transfer.

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Re: Ask Quick Questions, Get Quick Answers [Re: Coconutz]
    #28825207 -

Coconutz said:
Have 2 questions:
I made a small mistake and got tubs that don't have clear lids. Is it really necessary to cut hole in the top and put cling film wrap on it to let light in, or is light for the side of tubs fine?
Also how important is it to use 6000k lights/ sunlight. What effect would using a normal LED bulb have? I'm having a hard time finding consistent info on light, I feel like I'm missing something.


I have tubs with white plastic lids that are not light permeable, it's fine.  There just needs to be some light close to them in the room.  Some people swear by spectrum, but I just use whatever is around.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 1
    #28825210 -

@Coconutz

:whathesaid:

...also have White lids. And  any light source you could use to read the fine print easily. Don't overthink  it

Edited by LewDoja (06/24/24 10:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Pennock] * 1
    #28825213 -

They all look suspicious to me but need better quality photos  to say for sure.  I pick up an inexpensive lighted jewelers loupe that help tremendously to differentiate "hidden" mold

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #28825217 -

Can you elaborate what you mean? I understand the textured background that Phaethon mentioned but I got my mini photo light and I tried to get a dark background on them, and got them in focus decently I thought. The mycelium(or whatever it is) also just doesn’t have much definition to it so idk if that’s part of the problem as well. Happy to hear tips for photos!

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Re: Ask Quick Questions, Get Quick Answers [Re: Pennock]
    #28825235 -

Pennock said:
How's this? I threw them on a sticky note cuz that was in reach but I can probably find a better spot if this is still too tough to make out.

As for #3, couldn't tell you for sure, but I'm sure it involves my lack of experience with agar, as well as my shaky, nervous hands lol





I'm not convinced that's clean cubensis mycelium, but I'm also a noob (please take more experienced folks more seriously).

It's not a crazy amount of growth for 9 days (super fast growth is usually mold), and I'd expect mold to have sporulated by now...but the growth seems sort of random and squiggly instead of organized and uniform. I'm not that worried about the wispy leading edge - see that a lot with newly germinated cultures - it's how the filled in parts look that's suspicious.

Could see how it looks on water agar or a BRF puck, or just hang on to it while you germinate the next swab...


--------------------
LAGM 2026 grow log | My library/trade list

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Jump to top Pages: < First | | 1891 | 1892 | 1893 | 1894 | 1895 | 1896 | 1897 | 1898 | 1899 | 1900 | 1901 | 1902 | 1903 | 1904 | 1905 | 1906 | 1907 | 1908 | 1909 | 1910 | 1911 | | Last >

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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