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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28819607 -

Contamination spreads faster than mycillium.

At least in most cases. You will see it when you know what the warning sign are.


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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #28819639 -

BeefSupremeJr said:
wild bird seed (wbs) is a good option if you cant find a feed store.  search for a feed store via google map first though.  co-op feed store.  livestock store.  even cities have them because people have horses and llamas and stuff.


Oh shit I’m 5 minutes from a feed store, gotta go see what they got going on tomorrow. I have a huge bag of bird seed that’s been sitting in my garage for a couple years too but im not sure that would be clean enough to be spawn

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28819678 -

Hey so I bought some grain bags from mycohaus and I got some spore syringes and agar dishes I'm playing with.
I used some spore swabs and on some my MS solution to inoculate about 12 plates.

My initial plan was to knock my grain up with MS but after reading some more that's probably why all my tubs have gone bad in the past.

What's the best way to go from agar to grain bag? They have a SHIP but I'm not entirely sure how to exploit that with my agar.


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"I cannot begin to unravel the imagery that imposes itself during the finding of an orgasm. I jacked off eight times that night." Alexander Shulgin PIHKAL #20 2CB

Edited by Xiliad (06/20/24 02:37 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28819686 -

It'd probably be just fine as spawn. You'll be sterilizing it, and you can always rinse it if it's physically dirty. Some blends are probably better than others (e.g. corn can be too starchy and sunflower seeds don't really hydrate) but if you aren't planning to use it to feed birds, you may as well at least try it for spawn. Millet (typical main component of WBS) is easy to prep and pretty nice as spawn (especially for g2g donors) because there are so many tiny seeds in a given volume to act as inoculation points.

Wheat was a bit cheaper than millet at the feed store I went to, so I'm mostly using that. I also bought a bag of milo, and I'm not fully sold on it, mostly because the bag has so much chaff and other debris. Better for Muda bottles since it's mixed with coir at sterilization time (I used 2:1 coir:grain, was able to shake just fine after inoculation). I mixed it with wheat once, all boiled together, and I might try that again to see if I can get away without skimming off a bunch of debris.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Xiliad]
    #28819691 -

Xiliad said:
What's the best way to go from agar to grain bag? They have a SHIP but I'm not entirely sure how to exploit that with my agar.


Cut the bag open with a sterile scalpel, drop wedges in, seal the bag again.

That's really hard to do without sterile flow (flow hood or FFU). I wouldn't personally try it in a still air box.

If you can grow a clean agar plate, you can make liquid culture, and use the SHIP to inject a few mL from a syringe. EDIT: I just thought about LI (liquid inoculant). You can take a clean, colonized plate, add it to a jar of sterile water, and either blend or shake the shit out of it. Draw some of that into a syringe (might need a larger gauge needle than usual) and inject it into the bag via the SHIP. Faster than letting an LC grow out and probably less risk of major bacterial contamination.

Might be faster and safer to make a few grain jars yourself and drop wedges into them in a SAB. Save the bags for when you're more comfortable with sterile technique and have a nice clean LC. They should last for months.

You can use the syringes to inoculate agar plates (streak a drop with a loop or swab) or just do PF Tek (what spore syringes were designed for).


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LAGM 2026 grow log | My library/trade list

Edited by Phaethon (06/20/24 02:55 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28819697 -

Hey thanks for the reply. I might try a LI, but I'd still feel better just doing agar to a jar of spawn.

What's the consensus on doing LI this way? Would I only need to PC a jar of distilled water for 15 minutes and drop a clean plate in?

I have all the stuff for jars in the works anyway but don't want my bags to go to waste.


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"I cannot begin to unravel the imagery that imposes itself during the finding of an orgasm. I jacked off eight times that night." Alexander Shulgin PIHKAL #20 2CB

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Re: Ask Quick Questions, Get Quick Answers [Re: Xiliad]
    #28819700 -

If you guys have pre pressure cooked jars waiting for over 1 to 2 weeks, do you ever shake one or two to see if contains develop (while waiting for your plates to catch up)

I believe my grain is fine and I don't ever think I have contam based on procedure and equipment, but I didn't know if this would be a reasonable step of adding proof.


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My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente]
    #28819705 -

Kunta Kente said:
If you guys have pre pressure cooked jars waiting for over 1 to 2 weeks, do you ever shake one or two to see if contains develop (while waiting for your plates to catch up)

I believe my grain is fine and I don't ever think I have contam based on procedure and equipment, but I didn't know if this would be a reasonable step of adding proof.


If you had a blob growing in the middle, yes. Its happened for me.

I dont really shake, just kinda roll to make sure no lumps are sticking together.
But if 3 or 4 of a whole run are fine, I don't do them all unless i think there is some reason they'd be different


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Yours in the Natural State Land of Enchantment!

"Sometimes the only way for a man to find true happiness is to take drugs in a group" - Cochise

No makin funna my pomes!

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28819736 -

Arkyhog said:
That was the layer I put over the top of the blobs that were forming. I tried to pack it in a little bit but didn't get it that compacted. I will take a closer look at the "cotton ball"


Once the blobbs start to form the casing is a foregone conclusion. they wont magically turn into penis envy fruits. youve just buried perfectly good blobs.

GenesisCorrupted said:
Well, thank goodness. That you aren’t listening to their advice anymore.
:lol:
Once you mixed your sub and you’ve got it hydrated and closed the tub.

That’s it. You do not open it. Do not add anything to it. You do not spray, fan or add any more layers.

You got bad advice.

What you can do is control fae from outside by keeping tape on the holes or taking tape off of the holes.

But we don’t open the tub once they’re done they’re done. Wait until it’s finished.

Those blobs were Perfectly viable I don’t know why you would bury them.


I open my lids occasionally to take photos. when i do that; i lift the lid vertically without tilting. in this way i can wipe all of the water droplets off with a paper towel rather than wet my substrate further. if your substrate has so many water droplets that pooling has begun, gently dab with a paper towel. replace lid. voila. no fanning, mist on an as needed basis. most of the time this is because of improper field capacity of coir. When sizing a casing, hydration is especially important. i mist my casings until they look very slightly "muddy" if they appear dry. this is because the peat moss and vermiculite do not retain moisture efficiently as coco coir.


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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente]
    #28819781 -

Oh and how often do you replace your unmodded metal lids? Do you guys buy anything different? I notice after a couple of runs mine end up with rust spots. Dunno if those matter.


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My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente] * 3
    #28819811 -

I replace metal lids as soon as I get them with 1 piece plastic lids :shrug:  Rust on the surface of a lid isn't a big deal, but if it's on any of the sealing faces, it will allow stuff to enter the jar.

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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
    #28819868 -


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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28819894 -

I take it duct tape can be used in place of SHIPs when making spawn bags, right?
Don't feel like driving an hour to buy them, and figured duct tape should work, and then duct tape after the injection?
Thanks


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Re: Ask Quick Questions, Get Quick Answers [Re: fnulnu]
    #28819896 -

It works for bags and it works for jars.

Considering silicone works just was well, do it.

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Re: Ask Quick Questions, Get Quick Answers [Re: fnulnu]
    #28819898 -

wouldnt the glue melt during the cycle?


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #28819900 -

You can use packaging tape so surely duct tape would be okay?

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28819903 -

Huskies said:
Running out of my 50lbs millet bag. What y'all using for grain nowadays?


WBS, cheap (Cheep?:lol:) and never fails.


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New to cultivation or the shroomery?  Observe the hitchhiker's guide

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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 2
    #28819904 -

:shrug:

i used micropore tape once to keep the bags folded down during the cycle and the glue melted and everything was sticky. the tape fell off and was floating in the water.

thats the only basis for my comment.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 1
    #28819908 -

mushboy said:
wouldnt the glue melt during the cycle?


No, they were already sterilized, for 3h.


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Re: Ask Quick Questions, Get Quick Answers [Re: fnulnu]
    #28819910 -

:facepalm3:

ill see myself out.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Boomr Bag   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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