FriedEgg said:

weird. if it's jumping up and down then maybe it's actually holding pressure. if it's just hissing a little and it's not blasting out steam like in that video, then it's probably holding some kind of pressure.

so if you think it's holding some pressure but you don't know how much, i would just cook for 2-3 hours just to be safe. even if it only gets up to 10 psi, you can still sterilize if you do it long enough, it's just not ideal. but if you have no other option, you might as well

i wish i could find that sterilization time chart of different psi so you could confirm exactly how long you need to cook. does anyone know where that is?

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tryptkaloids said:

Those filters are most likely the cause. I wouldn't trust
Those to hold back grass seed, let alone mold.

You can claim your agar was clean until your dying breath, but I wouldn't confirm that until I saw a picture.

Also, steaming goes a long way towards sterile

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Nanutzu said:

It's definitely building pressure, the valve is releasing steam at half of the speed in the video.

(Wouldn't that be considered venting? while cooking.)

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FriedEgg said:

Nanutzu said:

It's definitely building pressure, the valve is releasing steam at half of the speed in the video.

(Wouldn't that be considered venting? while cooking.)

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no, here's how to vent properly:

open the valve on your PC. heat it. let steam continue to go out for 5 minutes while you're heating it. then close the valve and let the pressure build to maximum. then open the valve and let all the steam go out until it reaches 0 psi or it slows down. then close the toggle and let it get to max pressure. cook for your desired time.
the purpose is to expel all the AIR inside your PC because air will not sterilize your items. we need STEAM. if you don't vent the air out of your PC, there will be little pockets of air that will cause partial sterilization.

there's a lot of info about sterilization here: http://www.frankshospitalworkshop.com/equipment/autoclaves_equipment.html


but yeah, to summarize, you need to:
- hydrate your grain by simmering it until the inside is plump with moisture
- get proper filters for your jars
- sterilize at a high enough psi and for long enough time
- do all the other things correct like clean agar culture, be sterile, etc. but the mycelium in your jar looks fine IMO so i think your agar is probably clean.

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DERRAYLD said:

Ok but you didn't pc your agar, did you boil it for long enough to sterilize it?

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stockw said:

Hi to all

I've been dabbling with SSLC recently, this is a QQ for SSLC heads:

Do you find that if mycelium is growing on the top layer, you're in the clear? Or is that in no way an indicator of clean LC?

I dropped my wedges so that they sat on the top layer & I didn't shake or swirl with the hope that seeing clean growth would mean it's clean, similar idea to an agar dish, but it's probably not that simple eh

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huey.bluey said:

Im thinking of cloning this more normal looking melmac. the blob looks cool though.

also a p nat pin

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Pnin said:

QQ: Could you use a plin to streak a plate? This guy wouldn't come off the scalpel and I'm seeing growth where I tried to get it off before it fell to its resting place.

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Nerex said:

While transfering inside the SAB and after getting the little wedge, do you put the donor's petri dish cover back on it? I mean while you plan to keep taking more transfers from the donor.

When taking the little transfer, where do you put the cover for those seconds? On a paper towel soaked with ISO 70% is OK?

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