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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #28327302 -

Approximately how many agar plates will I need if I’m focusing on two strains, starting from spores?


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 2
    #28327311 -

Mycodemus said:
Approximately how many agar plates will I need if I’m focusing on two strains, starting from spores?



as few as 6 to as many as you want, if you were hunting isolates you could use 100's

if your spores are clean and you want to do a ms grow you only need a couple transfers after germ

if you're gonna take clones then at least 2 more per clone


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Re: Ask Quick Questions, Get Quick Answers [Re: AyePlus] * 2
    #28327317 -

Cool, thanks guys. Sounds like I can ditch the gasket if I choose to.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: AyePlus] * 1
    #28327319 -

AyePlus said:
Mycodemus said:
Approximately how many agar plates will I need if I’m focusing on two strains, starting from spores?



as few as 6 to as many as you want, if you were hunting isolates you could use 100's

if your spores are clean and you want to do a ms grow you only need a couple transfers after germ

if you're gonna take clones then at least 2 more per clone


If I understand correctly, I’d start with at least 6 plates per strain with spores, then go from there to clean it up?

(Love the ingenuity of homemade tools here btw, I never fail to be impressed by the humans of shroomery)


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao] * 1
    #28327328 -

The Tao said:
A bit of wire and a piece of dowel. The ashtray is perfect for keeping the loops off the surface and they can’t roll.


Ashtray you said :rofl:
Where is he?

    :cookiemonster:


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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus]
    #28327331 -

How long, on average does it take for your agar plates to colonize after a transfer (I know it can be species/variety dependant) just looking for a rough idea how long my cups will take so I can plan my next steps. Have B+, GT, APE6 and Tidal wave going now.


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: Ask Quick Questions, Get Quick Answers [Re: Goatrider]
    #28327336 -

161?
Goatrider said:
The Tao said:
A bit of wire and a piece of dowel. The ashtray is perfect for keeping the loops off the surface and they can’t roll.


Ashtray you said :rofl:
Where is he?

    :cookiemonster:


161? I liked him. He was spunky.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 2
    #28327344 -

Mycodemus said:
AyePlus said:
Mycodemus said:
Approximately how many agar plates will I need if I’m focusing on two strains, starting from spores?



as few as 6 to as many as you want, if you were hunting isolates you could use 100's

if your spores are clean and you want to do a ms grow you only need a couple transfers after germ

if you're gonna take clones then at least 2 more per clone


If I understand correctly, I’d start with at least 6 plates per strain with spores, then go from there to clean it up?

(Love the ingenuity of homemade tools here btw, I never fail to be impressed by the humans of shroomery)



I do 2 samples (spore or LC or whatever), 4 T1s, and then as many T2s as I have jars. So for you that would be (12 + x) plates | x = desired grain jars.

6 plates per generation per culture is overkill in my opinion but if you have the gear and time I don't see why not. It's a fun hobby and agar is cheap.


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Expanding my mind until I can join the collective

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Re: Ask Quick Questions, Get Quick Answers [Re: herbstation]
    #28327372 -

How are these shoeboxes looking?
1: bulk April 21 (someone here said last week it was highly suspect, but no green visible anywhere)
2: bulk April 22
3: bulk May 14 (the white dots were more obvious but I misted it yesterday and they faded a bit. The top layer was pretty thick, but I used my bare hands and bits of the shredded PF definitely got into the top layer)



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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #28327374 -

The Tao said:
161?
Goatrider said:
The Tao said:
A bit of wire and a piece of dowel. The ashtray is perfect for keeping the loops off the surface and they can’t roll.


Ashtray you said :rofl:
Where is he?

    :cookiemonster:


161? I liked him. He was spunky.


Yeah.
I even miss him.

    :cookiemonster:


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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 2
    #28327377 -

Looking good brotha #3 looks a little dry but the last pic looks rehydrated if that's the most recent one after you sprayed

You're a fungus farmer


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Expanding my mind until I can join the collective

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Re: Ask Quick Questions, Get Quick Answers [Re: herbstation] * 1
    #28327394 -

herbstation said:
Looking good brotha #3 looks a little dry but the last pic looks rehydrated if that's the most recent one after you sprayed

You're a fungus farmer


#3, I sprayed yesterday, took the pics just now. Actually I misted everything yesterday. My camera doesn’t really pick up details but 3 doesn’t seem dry irl.

I hope it’s the good kind of fungus haha πŸ˜…


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

Edited by Mycodemus (05/20/23 12:12 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus]
    #28327459 -

How long does it take for a cap to drop spores for a spore print?

The veil just broke overnight, cut the cap from the stipe and placed onto tin foil inside of my sab


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Citizen Mycologist:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: FungiSaves]
    #28327464 -

I find they usually drop well if I leave them overnight.


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 3
    #28327469 -

Mycodemus said:
Thanks guys, good to know! Will see what I can make from stuff I have at home.

Once you flame it, and it’s cooled, I assume by putting it into the agar, how do the spores stick to it? Is the agar cooling enough of a β€œglue”?


Cool it on the edge of the receiving dish.  The loop will be covered in sticky agar that can be used to pick up spores when you drag/touch it to your print.

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Re: Ask Quick Questions, Get Quick Answers [Re: sprocket]
    #28327484 -

sprocket said:
I find they usually drop well if I leave them overnight.


Thank you! I've heard that pe can sometimes not drop them?


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Citizen Mycologist:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710] * 1
    #28327486 -

PBJ710 said:
Mycodemus said:
Thanks guys, good to know! Will see what I can make from stuff I have at home.

Once you flame it, and it’s cooled, I assume by putting it into the agar, how do the spores stick to it? Is the agar cooling enough of a β€œglue”?


Cool it on the edge of the receiving dish.  The loop will be covered in sticky agar that can be used to pick up spores when you drag/touch it to your print.


I always feel a bit shitty when I put a loop with agar on the print. Feels bad.


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Ask Quick Questions, Get Quick Answers [Re: altford78]
    #28327494 -

So I have some jars that have some uncolonized grains at the very top. They've had ample time. No sign of trich in that batch.

What is the issue there? THey don't look wet either. Should I just scoop them out before shaking and putting it to bulk?


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: FungiSaves]
    #28327505 -

FungiSaves said:
sprocket said:
I find they usually drop well if I leave them overnight.


Thank you! I've heard that pe can sometimes not drop them?


Most PE won’t drop spores. They’re best collected via swabs.


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TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW] * 1
    #28327509 -

HILLBILLY OUTLAW said:
FungiSaves said:
sprocket said:
I find they usually drop well if I leave them overnight.


Thank you! I've heard that pe can sometimes not drop them?


Most PE won’t drop spores. They’re best collected via swabs.


What he said.  I didn't realize you were working with PE.


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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