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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: RE: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #28324410 -

mushboy said:
i had to reread that like 7 times:flowstone:


It happens :rofl:


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How I grow mushrooms 🍄
How I do agar 🦠

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Re: Get Quick Answers [Re: BumbleBeeTuna]
    #28324413 -

BumbleBeeTuna said:
Is there any correlation between "excessive" overlay/tomentose mycelium and pinning/fruiting? I'm looking at this tub and it seems like the substrate that didn't show as much initial mycelium growth (2nd pic, right side, where the cluster is) and the areas of less mycelium overlay is producing pins or fruit more quickly, as opposed to the areas with heavier mycelium growth that look "fluffy" (3rd pic, left/upper side). Anything I should be doing?








There is no overlay there, just fluffy myc. (Go check the Natalensis thread for overlay pics) It is normal to get spotty growth with MS grows. Cloning can get you better pinning and canopy tubs. I have seen canopies from MS on occasion. The empty spots may fill in on later flushes too. The fluffy myc seems a bit dry, maybe it's newer growth? You didn't do anything wrong. All you can do is watch them.


--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency





Edited by Bigworm (05/18/23 08:24 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69] * 3
    #28324415 -

rumfor69 said:
Fixing the title



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Links For Beginners And Beyond:mspoil:

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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69] * 1
    #28324436 -

Any EU heads got a hook up for borosilicate bottles? :shiftyeyes:

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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] * 1
    #28324439 -

I found mine on home brewing related website. Maybe you can find those in a similar themed store.

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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] * 1
    #28324441 -

What kind of bottles exactly? I get my (boro) glass stuff from heliosholland.com


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How I grow mushrooms 🍄
How I do agar 🦠

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Re: Ask Quick Questions, Get Quick Answers [Re: Throwaway] * 1
    #28324496 -

Ingushkaya said:
I found mine on home brewing related website. Maybe you can find those in a similar themed store.


cool i'll have a dig, cheers mate.

Throwaway said:
What kind of bottles exactly? I get my (boro) glass stuff from heliosholland.com


the lab style thick plastic lid & straight sides kind. I have erlenmeyers in that style but they're a bit shit to hold.

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Re: Get Quick Answers [Re: Bigworm]
    #28324527 -

Bigworm said:
There is no overlay there, just fluffy myc. (Go check the Natalensis thread for overlay pics) It is normal to get spotty growth with MS grows. Cloning can get you better pinning and canopy tubs. I have seen canopies from MS on occasion. The empty spots may fill in on later flushes too. The fluffy myc seems a bit dry, maybe it's newer growth? You didn't do anything wrong. All you can do is watch them.


Thanks for the advice. I do plan on collecting spores and working from agar for future grows, just getting this initial one out of the way. Per usual, pics don't do the surface justice but there's tiny beads of water throughout. I'll keep an eye on it, thanks!

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Re: Get Quick Answers [Re: stockw] * 1
    #28324562 -

stockw said:
altford78 said:
Shrimps said:
I personally would cut it into 4 pieces and dump those into the jar.


That's exactly what I'm thinking of doing, I just don't really know whether putting the edge in the jar is a no-no.

To be honest, I don't fully understand why the edge of the agar is considered more dangerous than the rest. How can it harbor invisible contaminates?

Or is it the edge of the actual plate,I mean the actual wall, that could have hidden contaminates?

:confused:


From my experience when I've had plates that've been sitting around a while, every now & then they get bacterial spots on the edges. I suppose it's the weak spot of a plate.

Why risk it? I'd just take what you can see it clean mycelium for peace of mind.


If your plates were sterile, poured and wrapped or otherwise prepared properly there should be no contamination on the plate. Is the circumference of the plate generally a weak spot? Maybe so, but it shouldn't be commonplace, unless you're picking something up somewhere or inadvertently exposing the agar to open air (improper wrapping) Are you getting this a lot on your plates?

It's easier to just cut the dish into pieces and either dump in or manipulate a single piece with a scalpel. I cut my agar into strips when using petris; each plate gives me 3-4 strips to inoculate with. I am currently using cups, so I cut them tic-tac-toe and dump them in after I separate the puck from the basin. Sometimes I do it when they're only 25% colonized or so. Either way, uncolonized agar shouldn't be something to worry about during inoculation unless there's another vector responsible.

If you are confident in your sterile technique and you want to cut it out of the middle i don't see the harm, just make sure not to take extra long or hover over it with your arms or hands as that increases exposure. It's all about knowing the vectors and mitigating your risks.


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⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions Here [Re: fiddle_head]
    #28324594 -

Is it bad practice to mix agar in a larger container and transfer to media bottles to PC or better to mix each media bottle individually?


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Yeah, yeah.

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Re: Ask Quick Questions Here [Re: NFLProof] * 3
    #28324601 -

Literally doesn’t matter as long as you’re PCin after it and understand ratios.


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Help US help YOU TEK

2023 Dehydrator TEK

Edited by Screwup (05/18/23 12:10 PM)

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Re: Get Quick Answers [Re: fiddle_head]
    #28324609 -

Nah it's fairly rare but does happen. I'm pretty slap dash when I'm wrapping my plates, could be that.

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Re: Get Quick Answers [Re: stockw]
    #28324663 -

How bad is it if I load my jars with moist grain? I fucked up and run out of sunlight so my grains are just sitting in colanders. No point in spreading them out to dry now.

I've been shaking the living fuck out of them but there's no way they will be dry. I mean they are not dripping wet but you know, moist grain sitting in a colander kind of situation.


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Get Quick Answers [Re: altford78]
    #28324673 -

altford78 said:
How bad is it if I load my jars with moist grain? I fucked up and run out of sunlight so my grains are just sitting in colanders. No point in spreading them out to dry now.

I've been shaking the living fuck out of them but there's no way they will be dry. I mean they are not dripping wet but you know, moist grain sitting in a colander kind of situation.


You want to spread them out so as much grain surface area as possible is exposed to the air. You can use a box fan if you have one or just lay them out on a screen if some sort. What grain are you using?


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Get Quick Answers [Re: Nichrome]
    #28324676 -

I opened my tub for a suprise this morning. Does this look like rot or mold to anybody? Not sure what’s going on here, feel doink.

The tub was sitting on the bottom so I can’t help but feel this is related to FAE. 2nd flush.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Get Quick Answers [Re: fiddle_head]
    #28324686 -

fiddle_head said:
altford78 said:
How bad is it if I load my jars with moist grain? I fucked up and run out of sunlight so my grains are just sitting in colanders. No point in spreading them out to dry now.

I've been shaking the living fuck out of them but there's no way they will be dry. I mean they are not dripping wet but you know, moist grain sitting in a colander kind of situation.


You want to spread them out so as much grain surface area as possible is exposed to the air. You can use a box fan if you have one or just lay them out on a screen if some sort. What grain are you using?


Wheat.

I guess I could spread them on a baking sheet even if it's dark. Can't find my screen so cookie sheet it is.

EDIT: Fuck it, in the fridge they go. It's late.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

Edited by altford78 (05/18/23 02:30 PM)

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Re: Get Quick Answers [Re: fiddle_head] * 1
    #28324691 -

fiddle_head said:
I opened my tub for a suprise this morning. Does this look like rot or mold to anybody? Not sure what’s going on here, feel doink.

The tub was sitting on the bottom so I can’t help but feel this is related to FAE. 2nd flush.


Looks like Pseudomonas.


--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency





Edited by Bigworm (05/18/23 01:55 PM)

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Re: Get Quick Answers [Re: Bigworm]
    #28324706 -

For fruiting in these shoeboxes (10 liters) would you let the lid closed or upside down ?
For best FAE

Lid closed



Lid upside down


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Re: Get Quick Answers [Re: Bigworm]
    #28324713 -

Bigworm said:
fiddle_head said:
I opened my tub for a suprise this morning. Does this look like rot or mold to anybody? Not sure what’s going on here, feel doink.

The tub was sitting on the bottom so I can’t help but feel this is related to FAE. 2nd flush.


Looks like Pseudomonas.


Would you harvest any of these or just say toss the whole thing?


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Get Quick Answers [Re: fiddle_head]
    #28324729 -

How long after inoculation can you reliability test LC?


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Avatar by Sol_Exposure

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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