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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: keylime123]
    #28310756 -

Only if you know how to go down sym drones!

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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28310776 -

What do you do with that? Squirt the syringe into the
San Pedro Girl said:
Proposition 122 said:
How do you get a drop or two out of a syringe? I pushed gently then about a cc squirted out :blush:


2ml pipette with pump.πŸ˜‰



What do you do with that? Squirt the syringe into the pipette?

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 2
    #28310777 -

Proposition 122 said:
How do you get a drop or two out of a syringe? I pushed gently then about a cc squirted out :blush:



Get a small piece of paper towel with your donor plate unwrapped.

Attach the leur lock needle. Slowly push down the plunge over the PAPER TOWEL and let the first bit shoot out.

The following very light pressure will allow a single drop to come out because you have already got rid of the pressure in the syringe onto the paper towel.

If you really want to practice get an empty syringe and experiment with water.

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #28310812 -

Proposition 122 said:
What do you do with that? Squirt the syringe into the
San Pedro Girl said:
Proposition 122 said:
How do you get a drop or two out of a syringe? I pushed gently then about a cc squirted out :blush:


2ml pipette with pump.πŸ˜‰



What do you do with that? Squirt the syringe into the pipette?


I don’t use syringes any more. I use it to squirt sterile water onto the spore print and suck it back up with the spores.


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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 5
    #28310822 -

Proposition 122 said:
How do you get a drop or two out of a syringe? I pushed gently then about a cc squirted out :blush:


Just push the barrel, so a drop forms at the needle tip.
Release it on the agar then.

Another tip is using the index finger as a break,
while pushing the plunger.



            :cookiemonster:


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Re: Ask Quick Questions, Get Quick Answers [Re: Goatrider]
    #28311036 -

can i use perlite instead of vermiculite doing pf tek?


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the pen is on the table
the penis on the table

Post Your Cultivation Picture of The Day
Ask Quick Questions, Get Quick Answers
A Huge List of Psilocybe Cubensis Variety description

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Re: Ask Quick Questions, Get Quick Answers [Re: danto] * 2
    #28311038 -

No


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: danto] * 2
    #28311059 -

But you can use coir (watertub tek)

edit: Assuming you were exclusively talking about the roll. ( double edit: bah you caught me before I could edit haha)

Edited by Kunta Kente (05/08/23 08:35 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente] * 1
    #28311060 -

i believe he was asking about using perlite instead of verm in the cake recipe.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl] * 1
    #28311070 -

San Pedro Girl said:
Nonagon said:
My little dehydrator is maxed out and will be running for a couple days at this rate.

If I were to cut an appropriate sized hole in a plastic container (Bucket? Tote?) to expand that mf’er and fashion some trays for it - would there be any noticeable difference in run time? Or is 24hrs still the golden standard

One guy here just chucks them in the bucket with no additional separation and apparently it works fine? Anyone try this?


https://www.shroomery.org/forums/showflat.php/Number/28218645#28218645


One(maybe more) of the trusted vendors sells additional trays that can be added to many common dehydrators.  You can do up to six trays, according to the manufacturer.  I've also put my dehydrator on a bucket and just let it run.  The mushrooms tend to stick together, but it works.

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Re: Ask Quick Questions, Get Quick Answers [Re: KeyMaker]
    #28311110 -

Is the cultivation of ATL #7 similar to P cyans?

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Re: Ask Quick Questions, Get Quick Answers [Re: DigDug]
    #28311113 -

Whats the pitfalls of WBS and what do you want to avoid? I feel like it was high concentration of corn or sunflower seeds. I feel like there's something to avoid. Thanks.


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Yeah, yeah.

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Re: Ask Quick Questions, Get Quick Answers [Re: DigDug]
    #28311115 -

DigDug said:
Is the cultivation of ATL #7 similar to P cyans?


No, much easier. Closer to cubensis


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28311129 -

Quick question:  Could an ovoid be crossed with a nat?

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Re: Ask Quick Questions, Get Quick Answers [Re: NFLProof] * 3
    #28311130 -

NFLProof said:
Whats the pitfalls of WBS and what do you want to avoid? I feel like it was high concentration of corn or sunflower seeds. I feel like there's something to avoid. Thanks.


WBS needs a good wash. Some brands are coated with an oily vitamin mix that can cause clumping. I wash mine maybe 5 or 6 times and I’ll put a drop or two of gentle soap in there to lift it all off. Out of six jars I still usually get one or two with a gumball sized clump in it. Avoid the brands with corn. Sunflowers aren’t that bad, but if you want to get rid of them they will float and you can simply pour or scoop them off.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28311175 -

As per smellys advise - WBS comes filthy, wash that shit thoroughly.

The small grains will get everywhere. If prepping more than a mesh strainer can handle, get yourself a piece of window screen and build one for over your sink.

Mixes with corn will be stickier, sunflowers will largely float and get poured off while rinsing

Less is more when it comes to heat. Boil water, turn off heat, then dump in rinsed/soaked grain. Or steep in hot/not boiling water until satisfied

Either way I like to briefly submerge in boiling water at the very end and lay it out flat so that the surface moisture steams off quickly on the strainer 

Extra water in the jars will get absorbed and hydrate the grain further during sterilizing. Some teks account for this, but I don’t. YMMV


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ… πŸ…„ πŸ„½ πŸ„² πŸ„· πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: Nonagon]
    #28311188 -

What's a baby boomer called?

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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup] * 3
    #28311207 -

Screwup said:
Is it possible to have increased contam rates if I don’t clean out my Petri dishes at all( with soap or water that is), dump agar in them, and then run them through the PC for 25-30 mins @ 15 PSI with 15-20 min venting?


Yes, the biofilm that accumulates is harder to penetrate. And any residual biology will need to be elimante therefore increasing time needed for effective sterilization. There is also the matter of exudates that are heat stable and can remain post pc cycle

Likely since those times are already padded you’ll be fine, and your dishes probably arent caked on with biofilm, but you are 100% theoretically reducing your chances of successful axenic cultures.  In practice :shrug: why waste Weeks over skippin minutes of workz.


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Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: AyePlus]
    #28311218 -

AyePlus said:
Screwup said:
Is it possible to have increased contam rates if I don’t clean out my Petri dishes at all( with soap or water that is), dump agar in them, and then run them through the PC for 25-30 mins @ 15 PSI with 15-20 min venting?


Yes, the biofilm that accumulates is harder to penetrate. And any residual biology will need to be elimante therefore increasing time needed for effective sterilization. There is also the matter of exudates that are heat stable and can remain post pc cycle

Likely since those times are already padded you’ll be fine, and your dishes probably arent caked on with biofilm, but you are 100% theoretically reducing your chances of successful axenic cultures.  In practice :shrug: why waste Weeks over skippin minutes of workz.


Thank you for the answer! Perhaps I’ll try cleaning them with soapy water again as my no pours have been getting quite a bit on contamination. This could be due to poor practices and all the condensation has well though.


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28311221 -

KeyMaker said:
Quick question:  Could an ovoid be crossed with a nat?


Yes, but it would require high level breeding techniques and microscopy to confirm.. ghetto crossing/spore mixing would be unlikely to succeed.

Monokaryons would likely breed, especially with something added to the agar weaken the cell walls


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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