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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: bongoman] * 1
    #27952030 -

bongoman said:
Do you think the nutrient content in the actual agar wedges makes a difference in terms of longevity here? My understanding is that the lack of nutrition is what puts the mycelium into suspended animation.

I did read of someone placing a wedge of colonised nutrient agar on a plate of water agar and then taking wedges from the colonised water agar to the vials of distilled water so that no nutrition found it's way into the distilled water...

Also, would these vials be ok?



The nutrient content on the agar wedges is technically non-zero nutrition so yes it would technically make a difference, but you can minimize this by cutting the donor agar wedge more shallowly so as to minimize how much agar makes it into the vial.

In my experience this is a non-issue:  Any residual nutrients will just get used up in the long run and you'll eventually be left with a vial that has no nutrients left in it.

The vials in the link will work just fine, they're functionally the same thing as the ones used in the tek.


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Cryovial Tek
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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 4
    #27952652 -



:rockon:


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Psicomb]
    #27953201 -

Psicomvb said:


:rockon:


Great work Psicomvb! :cool:


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Edited by Mycoplex (09/16/22 07:12 PM)

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 2
    #27954801 -

This is super cool. My only question is - did anybody study for how long cultures stored this way can actually survive? How about different species?


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Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 2
    #27954810 -

i’ve seen older threads mention they’ve brought back cultures after a few years in distilled storage but im not sure on the exact amount of time the cultures stay viable. pretty sure the posts were mainly in relation to cubes so no idea about other species


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 1
    #27954828 -

Thanks for the write-up. Nice work, toggle favorite.


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 5
    #27954983 -

Adas said:
This is super cool. My only question is - did anybody study for how long cultures stored this way can actually survive? How about different species?



Read a paper a while ago where cultures were assessed after 20 years with good results IIRC.
Here is the researchgate link:

Preservation of fungi in water (Castellani): 20 years


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Baba Yaga] * 1
    #27955560 -

Awesome, thanks! :hug:


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 1
    #27955753 -

Castellani's = spores in distilled water right?


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Rusty2096]
    #27981918 -

I just received 500 x 2ml vials.

How full are you filling them prior to sterilisation?

And obviously lids are kept loose, a quarter turn or so?

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: bongoman] * 1
    #27988829 -

bongo, they are usually filled about 70-75% of the way with sterilized distilled water, with the lids kept slightly loose like you said.  I imagine you've already gotten around to trying this by the time I wrote this reply.


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex]
    #27999991 -

When you PC the vials, are the kids left loose? Or do you tighten them?

Should you only PC the ones you intend to use right away or can you have some sitting around ready to use?

Thanks, all!


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Forgive me, I’m just learning

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: PanMax] * 1
    #28001315 -

Awsome, will use this for storing exotic species.

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: PanMax] * 4
    #28001320 -

PanMax said:
When you PC the vials, are the kids left loose? Or do you tighten them?

Should you only PC the ones you intend to use right away or can you have some sitting around ready to use?

Thanks, all!


What are you doing with the kids, man? :feelsbadman:

Your question was answered in the post before yours.

Have some sitting it makes no difference.


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Being pissed on does not make you a real man.

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Guerrilla] * 1
    #28032195 -

Just got my Vials today. Got them from the place for yeast cultivating stuff. The vials have some kind of liquid in them with tiny glass pearls. Any Idea what that is?

Best I just toss it out and use it with sterilized distilled water. But for what are those tiny glass pearls? Are they just used for cryogenic freezing?



Edit: I did some research, it is as followed: When using these tubes for cryo freezing, you inoculate the solution (including those pearls) with whatever culture you want to store. Then the cap is closed and carefully mixed. After that the solution gets sucked out with a sterile pipette and closed again.
That way the colony is holt inside of those glasspearls and can be frozen by -20 to -80 degrees. With the liquid the Tubes would break because of the expansion of the ice.

Just thought I post my answer I found here.^^


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Edited by Shrimps (11/04/22 02:33 PM)

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Shrimps] * 1
    #28034803 -

anyone have a link for some in the US? Cant find out which are able to be PCed  on amazon.

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Screwup] * 1
    #28034813 -

What I just found:

https://www.thomassci.com/scientific-supplies/Cryovials-2ml

edit: actually all cryo-vials must be autoclavable. So I assume also the chinese ones on amazon should be fine. ("assumption")


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Edited by Shrimps (11/05/22 06:47 PM)

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Shrimps]
    #28034825 -

Shrimps said:
What I just found:

https://www.thomassci.com/scientific-supplies/Cryovials-2ml

edit: actually all cryo-vials must be autoclavable. So I assume also the chinese ones on amazon should be fine. ("assumption")


Yeah the Chinese ones don’t all specifically say. I found one that goes to 248F and then others don’t say…

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Screwup] * 1
    #28038260 -

I have used these. They went through PC cycle fine.

https://smile.amazon.com/gp/product/B08FKXQ3XR


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: ReverendMyc] * 1
    #28038292 -

ReverendMyc said:
I have used these. They went through PC cycle fine.

https://smile.amazon.com/gp/product/B08FKXQ3XR


I think these are the exact ones minus the orange tip that I just got. Awesome news. Thanks for the reassurance.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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