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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27345276 -

It's hard for me to tell if I trust it or not from the pics. Looks good around the edges for sure but I can't tell if I trust the middle.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27345282 -

verum subsequentis said:
It's hard for me to tell if I trust it or not from the pics. Looks good around the edges for sure but I can't tell if I trust the middle.


Cool. I got a fuckton of clone T3s and a fuckton of poured plates. I'll do some transferring later today. Think I can snatch a little wedge off the edge for an LC?


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27345303 -

verum subsequentis said:
It's hard for me to tell if I trust it or not from the pics. Looks good around the edges for sure but I can't tell if I trust the middle.


What is it that you don't trust about the middle? How would you want the middle to look? Sometimes I'm not sure about the color of the middle looking from the underneath side... if it's contam or just the combined color of the transferred piece and agar :shrug:


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Granny's Awesome LAGM21

>Things I wish I knew when I started<

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Threads2theSoul]
    #27345377 -

Tree of life

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Threads2theSoul]
    #27345395 -

Threads2theSoul said:
verum subsequentis said:
It's hard for me to tell if I trust it or not from the pics. Looks good around the edges for sure but I can't tell if I trust the middle.


What is it that you don't trust about the middle? How would you want the middle to look? Sometimes I'm not sure about the color of the middle looking from the underneath side... if it's contam or just the combined color of the transferred piece and agar :shrug:


That's a good question. the very center is a triangular agar wedge from T2. The little flecks everywhere is what food coloing does to my plates (next time I'm trying activated charcoal). If it is the surrounding central area I would be grateful for as much information as you care to impart. This here is The College of TEKnowledge.

The pictures are shot with an iPhone 12 Pro Max. Back-lit front and back on a 5200K light box and kinda bottom lit standing behid the lightbox.

I can re-shoot em with a D3mkIII adn a 100mm macro?

TY
Space


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby] * 3
    #27345570 -



Nat T1


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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #27346388 -

I know this is probably a very noob question, but I'm having some trouble seeing any growth from a transfer and it's a little frustrating.

The transfer I took it from looks clean and grew out reasonably quickly, but I took 3 or 4 samples from it and none of them have any signs of growth at all. I was wondering if this is normal or why this could happen? I transferred after about two weeks, when it was about 5-6cm in diameter, was this too late?

I'm using the same recipe as before, and one other transfer (from a different strain) is growing normally.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: glive]
    #27346390 -

glive said:
I know this is probably a very noob question, but I'm having some trouble seeing any growth from a transfer and it's a little frustrating.

The transfer I took it from looks clean and grew out reasonably quickly, but I took 3 or 4 samples from it and none of them have any signs of growth at all. I was wondering if this is normal or why this could happen? I transferred after about two weeks, when it was about 5-6cm in diameter, was this too late?

I'm using the same recipe as before, and one other transfer (from a different strain) is growing normally.


Pics would help... ive had a few occasions where I tried to grab a tiny piece of myc only to botch the scoop... once finally collected and transferred, it seemed to have been damaged and was unable to recover... just a thought...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27346396 -

roarkell said:
glive said:
I know this is probably a very noob question, but I'm having some trouble seeing any growth from a transfer and it's a little frustrating.

The transfer I took it from looks clean and grew out reasonably quickly, but I took 3 or 4 samples from it and none of them have any signs of growth at all. I was wondering if this is normal or why this could happen? I transferred after about two weeks, when it was about 5-6cm in diameter, was this too late?

I'm using the same recipe as before, and one other transfer (from a different strain) is growing normally.


Pics would help... ive had a few occasions where I tried to grab a tiny piece of myc only to botch the scoop... once finally collected and transferred, it seemed to have been damaged and was unable to recover... just a thought...


Ah, this could be it for sure. Although I remember these transfers went pretty smoothly, I've definitely botched it many times like you described. I also read once that you really only need a tiny piece to transfer, but I'm worried it could be too small? Usually I cut a triangle about 1cm big.

If it helps, here's a pic..

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Re: AGAR ENVY! (Anything and All things agar!) [Re: glive]
    #27346410 -

Hmm... that does seem odd... I would say those are big enough that they should have survived... you said your agar was the same batch/recipe? You didn't forget the nutes or anything?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27346419 -

roarkell said:
Hmm... that does seem odd... I would say those are big enough that they should have survived... you said your agar was the same batch/recipe? You didn't forget the nutes or anything?


Yeah, I made a small batch so it should have been:

3g agar
3g potato flakes
2g honey
0.3g yeast
150ml water

I copied this from somewhere here and it seems to work!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: glive]
    #27346542 -

glive said:
roarkell said:
Hmm... that does seem odd... I would say those are big enough that they should have survived... you said your agar was the same batch/recipe? You didn't forget the nutes or anything?


Yeah, I made a small batch so it should have been:

3g agar
3g potato flakes
2g honey
0.3g yeast
150ml water

I copied this from somewhere here and it seems to work!


That recipe seems very nutritious... id take out one of the nute sources (honey or potato flakes) and try again. If there's too much food, the mycelium has no reason to branch out. I like my mix to be about 2% total nutrition by weight...

I'd go with something like:
3g agar
3g potato flakes
(idk about yeast, never tried it)
150ml of water

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Re: AGAR ENVY! (Anything and All things agar!) [Re: glive] * 1
    #27346569 -

glive said:
If it helps, here's a pic..



It would appear your tissue samples are encountering bacteria....does anyone else see that or am I fucked up? Viewing from my phone so I could be :shrug::lol:


--------------------
Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27346622 -

Tormato said:
glive said:
If it helps, here's a pic..



It would appear your tissue samples are encountering bacteria....does anyone else see that or am I fucked up? Viewing from my phone so I could be :shrug::lol:


I kinda thought that on the middle dish but I think it's just water spots on the lid...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27346631 -

You can't see it too easily in the photo, but there are some faint sort of milky patches in some of the dishes too. I'm pretty sure it was this batch and when I think about it, I'm pretty sure I fucked up and re-used some plates that I had washed using just soap and water. I'm not sure if I mixed unused ones in there as well though...

Thanks though, roarkell! I've put some new plates in the PC without the honey so I'm hoping for the best. I'm also going to try and put a wedge in to grain and see if it grows.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: glive] * 1
    #27346633 -

glive said:
You can't see it too easily in the photo, but there are some faint sort of milky patches in some of the dishes too. I'm pretty sure it was this batch and when I think about it, I'm pretty sure I fucked up and re-used some plates that I had washed using just soap and water. I'm not sure if I mixed unused ones in there as well though...

Thanks though, roarkell! I've put some new plates in the PC without the honey so I'm hoping for the best. I'm also going to try and put a wedge in to grain and see if it grows.


The donor plate looked fine... a wedge from that should get you going well...

And here's hoping your next plates are more successful! :cheers:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell] * 1
    #27346814 -

Seeing really nice growth on all plates, germination included. I had to throw out one germination plate as there was some serious yellow discoloration and the start of a milky film.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27346884 -

Trying out the big pp dishes, they don't have clear lids but they're easy to shine a light through.



Edited by DERRAYLD (06/13/21 04:39 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27346957 -

that + divided into 8 slices like pizza.

germ plate to 8 different transfers in one dish

:havesomescience:


First plate to show proper looking growth from like 140 plates across 6 varieties.



While i have my troubles and delays with getting a clean culture to run one of my 2 clone plates from a cluster from a failing PEU project started pinning just ~4-5 weeks invitro- took the first one to pop out. Happy to try growing this out while i find my way to being able to spawn to bulk properly- hoping its a little winner


Edited by moshe1 (06/13/21 09:13 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1] * 3
    #27347267 -

Friendly reminder that you can do this after pouring and just cut off what you need.


--------------------

Crack's Tek's and Shit


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