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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1] * 1
    #27330852 -

Should be ok for a short time.
I´d have wrapped the lids with cling wrap,
and placed them in ziplocks, then to the fridge.
Just an idea :wink:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Goatrider]
    #27330929 -

If it's not yet sterilized, think of it as you would food..

Bacteria will start multiplying within like 20 minutes (fucking crazy) and show visible growth within 12 hours or so. Mold will start showing growth within something like 2-14 days.

Letting a culture colonize your agar before sterilizing will change the composition of the medium, but regardless, once you sterilize it, nothing should grow except the culture that you introduce.


Best practice is to sterilize your agar right after preparing it. Then you have plenty of time to let it sit before actually pouring it. However, keeping things moving is always best practice. Prepare, sterilize, and pour your agar all within 3 or 4 hours is always best.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27330936 -

TheDoobsker said:
If it's not yet sterilized, think of it as you would food..

Bacteria will start multiplying within like 20 minutes (fucking crazy) and show visible growth within 12 hours or so. Mold will start showing growth within something like 2-14 days.

Letting a culture colonize your agar before sterilizing will change the composition of the medium, but regardless, once you sterilize it, nothing should grow except the culture that you introduce.


Best practice is to sterilize your agar right after preparing it. Then you have plenty of time to let it sit before actually pouring it. However, keeping things moving is always best practice. Prepare, sterilize, and pour your agar all within 3 or 4 hours is always best.


Just out of interest, is this your experience?
I have a cup of agar from 2 weeks back that is currently crystal clear, not pc'ed, just clean agar in a pp cup.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27330957 -

So bacteria naturally multiplies that quickly, but I think agar prep temps alone kills a huge amount of bacteria and probably even some mold spores.

I have prepped agar a few times and didn't sterilize right away and saw bacteria pretty quickly. But, my above statement come most from reading and learning from others


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27331077 -

APE on MYA



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27331199 -

That's a wild lookin plate.

I have PCed agar, gotten busy, left town, travelled the world, ran with the bulls... several other things I'm not proud of and won't mention... Only to come home weeks later, microwave that shit and then pour plates. Works like a charm if everything is done right. As always though, one wrong move and it's all shite.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27331342 -

I'm working hard to develop an eye for contamination on agar, I think that's where most of my current issues are.
I've got some hitch hikers here, 3 questions for the thread please

1st photo: It's the second time I've had this bacterial(?) ring around a plate. Is there a reason for this?

Sector 10-11 is this just weak mycelium or is something hiding in there?

2nd photo: looks like mold pretending to be mycelium, do I see a bluish tinge there?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27331370 -

The bacterial ring is usually from condensation sliding around spreading bacteria.


The myc looks good to me, that 10-11 spot is just where the two colonies are running into each other.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27331374 -

I have this transfer ready to be dropped to grain but noticed a minor creaminess which is assume is bacterial, just not certain it is.
Sorry but pp is a pita to take photos through.

Last plate is a transfer from a dirty print, you can see it running but I'm wondering if it has outgrown the contamination?
Any thoughts?
I will take it to a new dish anyway but enjoy the discussion.



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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27331482 -

xVandalx said:
I'm working hard to develop an eye for contamination on agar, I think that's where most of my current issues are.
I've got some hitch hikers here, 3 questions for the thread please

1st photo: It's the second time I've had this bacterial(?) ring around a plate. Is there a reason for this?

Sector 10-11 is this just weak mycelium or is something hiding in there?

2nd photo: looks like mold pretending to be mycelium, do I see a bluish tinge there?




The first photo obviously has the bacterial colony over there at 3 o'clock but everything else there looks fine...

The second pic, id take a few xfers from 9-11 o'clock and not worry about it... :shrug:

The bigger question is, what are you doing wrong that you're getting these satellite contams on t4...

Edited by roarkell (06/01/21 04:03 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27331483 -

D3monic said:
APE on MYA




Is MYA the same as MEYA?


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27331697 -

Yes, what am I doing wrong is the question, I have 4 other plates, 2 of each variety that appear clean (for now). I have a flow hood and a SAB, these transfers were done in the SAB because of the mold from the previous plates. I'm used to seeing mold, the bacteria is new to me.

Practice, practice, practice is my mantra

Working on my moves...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx] * 1
    #27331725 -

Satellite Contamination from making the transfer or even opening the petri. A tool or hand will do it.


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27331728 -

xVandalx said:
Yes, what am I doing wrong is the question, I have 4 other plates, 2 of each variety that appear clean (for now). I have a flow hood and a SAB, these transfers were done in the SAB because of the mold from the previous plates. I'm used to seeing mold, the bacteria is new to me.

Practice, practice, practice is my mantra

Working on my moves...


Looks like you've got a good handle on it... just gotta fine tune it... otherwise you're looking good bro!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27331788 -

Had some T3's and 4's from a germ plate that were less than ideal. Wasted almost 2 weeks trying to get them cleaned up all to just end up the same fluffy thick slow growth.
Went back to the germ plate and saw there was a tiny spot of rhizo growth climbing up the dish wall and made a new T1.

Now got 3 new T2's that look way better than previous transfers. Being my first grow ever and working with agar for roughly a month, I'm excited to be moving in the right direction.. for now! Hopefully be moving to grain soon


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Re: AGAR ENVY! (Anything and All things agar!) [Re: GandalfTheWhite] * 1
    #27331798 -

GandalfTheWhite said:
Had some T3's and 4's from a germ plate that were less than ideal. Wasted almost 2 weeks trying to get them cleaned up all to just end up the same fluffy thick slow growth.
Went back to the germ plate and saw there was a tiny spot of rhizo growth climbing up the dish wall and made a new T1.

Now got 3 new T2's that look way better than previous transfers. Being my first grow ever and working with agar for roughly a month, I'm excited to be moving in the right direction.. for now! Hopefully be moving to grain soon




You're developing an eye for it! Congrats! These T2's look promising!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PitcherCrab]
    #27331816 -

PitcherCrab said:
D3monic said:
APE on MYA




Is MYA the same as MEYA?


🤷🏽‍♂️ it’s basically my lme recipe but with 1g nutritional yeast added. Not sure the proper acronym


--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27331992 -

has anyone ever tried reducing nutrition in LME agar?  To something like 5G LME to 10G AGAR for a 400ML


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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27331997 -

Yeah, I've dropped mine pretty low. I'll go to 7-8g per 600ml. I'll do 10g agar, 7-8g lme and 1g yeast for 600ml.


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27331998 -

D3monic said:
PitcherCrab said:
D3monic said:
APE on MYA




Is MYA the same as MEYA?


🤷🏽‍♂️ it’s basically my lme recipe but with 1g nutritional yeast added. Not sure the proper acronym


Got it. Yah exactly the same thing! I’ve always called it MEYA (Malt Extract Yeast Agar), but I honestly  don’t know if thats right. I’m planning on making some of that next.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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