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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,007
Loc: South Africa
Last seen: 46 minutes, 50 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Southerner]
#27316130 - 05/20/21 12:10 PM (5 years, 5 months ago)

:mad:

Edited by DERRAYLD (05/20/21 12:11 PM)

Extras:

verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#27316252 - 05/20/21 01:46 PM (5 years, 5 months ago)

Bummer. How'd you pull that off? It's easily savable though, so that's nice.

Southerner, I can't tell a gosh darned thing from that photo. Standing water can and will cause the myc to look odd and make it hard to distinguish clean cultures from dirty ones.

--------------------
Masters and slaves hunting clones

How not to isolate (it worked, but)

Extras:

DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,007
Loc: South Africa
Last seen: 46 minutes, 50 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27316270 - 05/20/21 02:01 PM (5 years, 5 months ago)

I'm trying to figure that out at the moment.
Starting a new batch of agar tonight to make some transfers tomorrow, quite a few plates look like this unfortunately.

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Southerner
Stranger

Registered: 03/25/21
Posts: 611
Loc: South

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27316299 - 05/20/21 02:30 PM (5 years, 5 months ago)

verum subsequentis said:

Southerner, I can't tell a gosh darned thing from that photo. Standing water can and will cause the myc to look odd and make it hard to distinguish clean cultures from dirty ones.

Haha yeah it was a pain trying to get a picture with everything visible. If you expand the photo and zoom in on the transferred wedge you can see some squiggly growth that I’m assuming/hoping is pearl oyster myc. The bumpy area is at the very top of the plate but it’s not very visible.

Extras:

A.k.a
Stranger

Registered: 10/27/19
Posts: 18,226
Loc: Gaming the system
Last seen: 8 hours, 4 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#27316448 - 05/20/21 04:16 PM (5 years, 5 months ago)

I’d bet it’s from bumping your scalpel or something the way the bacteria seems to be mostly in a straight line across the plate.

Also I’m pretty sure that’s not oyster myc, looks like some weird tentacle :eek:

:eek:

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Southerner
Stranger

Registered: 03/25/21
Posts: 611
Loc: South

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27316678 - 05/20/21 06:37 PM (5 years, 5 months ago)

A.k.a said:

Also I’m pretty sure that’s not oyster myc, looks like some weird tentacle

Well what in fuckin’ tarnations is it then?! 😂 That’s the second time I’ve gotten that sort of ‘myc’ growth off of a plug a friend gave me. Other one looked like a translucent tree. Beautiful but sadly didn’t see it until I opened the plate to toss it. Figured that’s just how it was expressing itself on straight oat water agar.

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,007
Loc: South Africa
Last seen: 46 minutes, 50 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27317011 - 05/21/21 01:56 AM (5 years, 5 months ago)

A.k.a said:

I’d bet it’s from bumping your scalpel or something the way the bacteria seems to be mostly in a straight line across the plate.

I'm busy trying to figure it out at this stage, I've introduce too many new variables, tools,etc.
Stepping back to keep it simple and get it all clean again.

Extras:

verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#27317134 - 05/21/21 07:58 AM (5 years, 5 months ago)

Looks to me like you gleeked on it.
I think that's what it's called.

--------------------
Masters and slaves hunting clones

How not to isolate (it worked, but)

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,226
Loc: Gaming the system
Last seen: 8 hours, 4 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27317139 - 05/21/21 08:08 AM (5 years, 5 months ago)

Lmao yeah did you hold your tongue at a weird angle and spray contam??

I feel you man one of my biggest problems is changing a bunch of things at once and then I have no idea what’s causing things to happen and have to go through and test one by one.

--------------------

Extras:

verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27317141 - 05/21/21 08:12 AM (5 years, 5 months ago)

fur realz.
Best advice ever, walk in the established path.
If you decide to switch thangs up, do it one thang at a time and only once you have gotten your sea legs.

--------------------
Masters and slaves hunting clones

How not to isolate (it worked, but)

Extras:

Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27317195 - 05/21/21 09:23 AM (5 years, 5 months ago)

I worked in quality control R&D for a while and that's the probably the most valuable bit of advice anyone can ever receive. Any changes or adjustments made need to be one at a time and in small increments while collecting data. When you work that way, you build a data trend that gives you a real time view of what your changes are actually changing. When you make big jumps or change too many processes, you get wild results and you don't know have a clue what it was that helped or hurt your end goal.

--------------------

Extras:

verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27317200 - 05/21/21 09:27 AM (5 years, 5 months ago)

10000000000000000000000000000000000000000000000%
Aspiring noobs should tattoo this backwards on their forehead and read it every day while brushing their teeth.

--------------------
Masters and slaves hunting clones

How not to isolate (it worked, but)

Extras:

DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,007
Loc: South Africa
Last seen: 46 minutes, 50 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27317215 - 05/21/21 09:40 AM (5 years, 5 months ago)

The problem I have is literally everything changed for me so everything is currently a variable that I'm trying to cancel out as vectors.

Extras:

verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#27317220 - 05/21/21 09:42 AM (5 years, 5 months ago)

I've been there.
Just dial everything in back as close as you can to what has worked before.
And carry on.
You got this.

--------------------
Masters and slaves hunting clones

How not to isolate (it worked, but)

Extras:

DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,007
Loc: South Africa
Last seen: 46 minutes, 50 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27317229 - 05/21/21 09:55 AM (5 years, 5 months ago)

I setup some control plates last night as well to check my pc times for agar, hopefully everything is ok as expected but I have to check everything now.

Extras:

Mycoctopuss
Mycoctopuss

Registered: 05/06/21
Posts: 103
Last seen: 4 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27317245 - 05/21/21 10:03 AM (5 years, 5 months ago)

I'm a pretty newbie in agar teks. I prefer Malt Extract Yeast Extract peptone Activated Carbone Agar. my Myceliums grow fairly well. most of them Rizho Growth and some of them are tomentose but very fast grows.
but I See Some Pictures of Agar Cultures From Experienced Growers on the internet they achieve an Extremely Ropey Tissue of mycelium.
I Use Lab Grade Ingredients and spend a lot of money on them. but I can't achieve that yet. do you have any advice on it?

Extras:

verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mycoctopuss]
#27317249 - 05/21/21 10:08 AM (5 years, 5 months ago)

My advice:
Quit searching for extremely ropey myc.
Ditch the lab grade expensive ingredients.
Use malt extract and agar at 2%.
Works for me.

--------------------
Masters and slaves hunting clones

How not to isolate (it worked, but)

Extras:

D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mycoctopuss]

1
#27317254 - 05/21/21 10:15 AM (5 years, 5 months ago)

Mycoctopuss said:

I'm a pretty newbie in agar teks. I prefer Malt Extract Yeast Extract peptone Activated Carbone Agar. my Myceliums grow fairly well. most of them Rizho Growth and some of them are tomentose but very fast grows.
but I See Some Pictures of Agar Cultures From Experienced Growers on the internet they achieve an Extremely Ropey Tissue of mycelium.
I Use Lab Grade Ingredients and spend a lot of money on them. but I can't achieve that yet. do you have any advice on it?

Keep it simple, I use amazon grade beer light malt extract, living jin brand agar.. thats it. Occasionally anyhonys premium yeast flakes. Non fortified.

Standard recipe

1000ml distilled water
20g agar
Anywhere between 14-17g LME.

If I want to switch things up I’ll add 1g yeast to the recipe.

Green in the pics is on yeast

--------------------

D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mycoctopuss]
#27317330 - 05/21/21 11:35 AM (5 years, 5 months ago)

Mycoctopuss said:

I'm a pretty newbie in agar teks. I prefer Malt Extract Yeast Extract peptone Activated Carbone Agar. my Myceliums grow fairly well. most of them Rizho Growth and some of them are tomentose but very fast grows.
but I See Some Pictures of Agar Cultures From Experienced Growers on the internet they achieve an Extremely Ropey Tissue of mycelium.
I Use Lab Grade Ingredients and spend a lot of money on them. but I can't achieve that yet. do you have any advice on it?

Ropey rhizo myc means nothing. You can have a killer tub from either tomentose or rhizo myc. Truthfully, I've gotten better pin sets from tomentose cultures than I have from my rhizo cultures. My Rhizo has been like that beautiful tall blonde with big tits. Good as fuck to look at but when you try to hold a conversation, you find out that she's dumber than shit. :shrug:

:shrug:

--------------------

Extras:

Summers
Newbie Grower

Registered: 05/21/21
Posts: 4

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27317589 - 05/21/21 03:34 PM (5 years, 5 months ago)

Reading this thread gave me so many good ideas and the confidence to buy and prepare agar...
So exciting!!
:headbanger:

:headbanger:

Extras:

Pages: < First | < Back | 438 | 439 | 440 | 441 | 442 | 443 | 444 | 445 | 446 | 447 | 448 | 449 | 450 | 451 | 452 | 453 | 454 | 455 | 456 | 457 | 458 | Next > | Last >

Shop:

Bulk Cannabis Seeds

Isolated Cubensis Liquid Culture For Sale

Spores for European Microscopy

North Spore Mushroom Grow Kits & Cultivation Supplies

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