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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Raccoon]
    #26002140 -

It looks pretty bacterial tbh. What's your process like?


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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Alchemycologist]
    #26002157 -

Alchemycologist said:
Bacteria leads to mold and low standards leads to bad growers.

To you I say raise your standards.


How you would know if "Bacteria leads to mold" unless you actually did fruit a jar with bacteria? In fact, to become a good grower you would actually have to try fruiting a bacterial jar to have the experience necessary to become a trusted cultivator. And it seems that bacteria does not lead to mold in many cases, so you are spreading bad information (or maybe just incomplete information). Bad growers (or any hobby, profession, etc) don't learn from mistakes and experiment, this is what you are advocating.


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First Grow

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Raccoon]
    #26002167 -

Honestly, just don't bother, the guys kinda a cunt it seems.


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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: southbounpachyderm]
    #26002174 -

I followed Bod's oat tek, agar tek, and everything to the T. You can see my Journal entry in my signature... I have had minimal contamination issues, but I have had them, so I am making mistakes here and there, but overall I'm doing all right.

All the jars have signs of this and it's unlikely the same contam came from my process in all 8 jars. Not impossible, but unlikely... I'm guessing the contam (if it is) came from the agar plates.  I only transferred once and the plates looked a little poofy in places... I was impatient. I have back up plates that I can clean up... I'm going to try to fruit and see what happens.


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First Grow

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Raccoon]
    #26002188 -

Cleaning plates up is the toughest thing when you're new. I'm still struggling with it at times, gaining a better eye for what's clean and what's what with time. Guess I should have just thrown all that spawn away instead of get the experience in though. Really fucked up there.

I like to wait until I see nice growth with no phantom spots. Growth that flattens out after the transfer. Too poofy shows me contams ime.


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Edited by southbounpachyderm (05/19/19 08:59 PM)

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: southbounpachyderm]
    #26002190 -

I know that bacteria leads to mold because I have 17 years of experience. I was a TC until I forgot my password.

There's no need to learn from mistakes that many, many others have already made.

Call me names all you want but I only advocate reliable methods, not spawning some half-assed bacterial crap. Making spawn isn't that hard. Use agar, do enough transfers to clean it up and spawn something clean instead of pulling some bullshit flush, thinking you did it right and never getting better.


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Yep.

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Alchemycologist]
    #26002202 -

That's all fine and good but it took me more than a few tries of bacterial spawn to get to somewhere I feel comfortable. Posting pics, learning from shared experience and personal experience, is imo integral to development in this hobby. I read for months before I even started and still didn't get a grasp until I worked further and harder personally. Maybe post some pics of what you consider nice and clean instead  :shrug:  :shrug:  :shrug:


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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Alchemycologist]
    #26002223 -

Quote:
thinking you did it right and never getting better.


No one said anything about never getting better. Fruiting from a slightly bacterial jar has nothing to do with not trying to prevent it from happening again.  Trying to fruit from this jar is not the mistake, the mistake was already made.


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First Grow

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Raccoon]
    #26002490 -

I wish I could reach through the screen, grab people's head and shake the shit out of it. This trend of non-scientific people who have half cocked opinions and always tell people to throw everything away is garbage, it produces garbage posts. Your privilege is showing.


Yup


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Professor X]
    #26002604 -

:shrug:

I stand by my previous statements.


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Yep.

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Alchemycologist]
    #26002618 -

I've harvested over a pound dry from "bacterial spawn" in the last month. Some are on their 5th or 6th flush. Spawned to CCVG, yes, coffee too. Trichoderma city right? Wrong. I'll explain it as non sciency for you as I can.

Bacteria, the little nasty booger we mostly hate (there are beneficial species too, sterilize some straw and shit you'll get a tub full of green.) is more aggressive than cube mycelium and eats the grain faster. Cubes won't colonize rotten or spent grains but Trichoderma will.

Bacteria is at a distinct disadvantage because it lacks the mycelial network so it can't move across the grains. 9 times out of 10 those few uncolonized putrid rancid bacterial grains that are stuck to the jar stay stuck to the jar even after shaking it with a sawzall paint shaker adapter. In the event you do get some bacterial grains in the sub, no sweat. You only run into problems when large amounts of the grains won't colonize.

Trichoderma is a natural part of the process, it comes in when the mycelium is done to finish the job. It's the natural process. It becomes problematic when you just let it go and it adapts then you spread it to other tubs and it wrecks all your shit.

  TLDR - to be effective and efficient in this hobby it pays to have an understanding and respect for the entire kaleidoscope of organisms you are dealing with and the natural life cycle associated with each.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Alchemycologist] * 1
    #26002635 -

Alchemycologist said:
:shrug:

I stand by my previous statements.


Rock on man. I don't distrust your experience, I just think we have a different view of the process. I'm here to learn, and spawning bacterial jars is a learning process. Anyway, I'm gonna put my jars to substrate and I'll update, good or bad. I'm actually a little pessimistic, but fuck it.


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First Grow

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Raccoon]
    #26002644 -

Don't be pessimistic about it at all. If you get fruits, excellent! If you dont, oh well, start a new project and learn from your past mistakes. Or just throw everything away and start over again. What do I know... Honestly, not much, enough to know that any experience is better than none.


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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: southbounpachyderm]
    #26002676 -

G2G it then give the jar a smell test.  That will give you a clue. More than likely you will get fruits.  If it was me though I would have a backup going just in case.

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Claybirdd]
    #26002765 -

Spawn anything to a shoebox, if it fails toss it in the garden.

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: YarnDi]
    #26002826 -

I've never tried shoeboxes.  27 quart monotubs is the smallest I've done.

Edited by Claybirdd (05/20/19 07:31 AM)

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: Raccoon]
    #26003025 -

Raccoon said:
I followed Bod's oat tek, agar tek, and everything to the T. You can see my Journal entry in my signature... I have had minimal contamination issues, but I have had them, so I am making mistakes here and there, but overall I'm doing all right.

All the jars have signs of this and it's unlikely the same contam came from my process in all 8 jars. Not impossible, but unlikely... I'm guessing the contam (if it is) came from the agar plates.  I only transferred once and the plates looked a little poofy in places... I was impatient. I have back up plates that I can clean up... I'm going to try to fruit and see what happens.


yup, my guess is that something is sneaking in with your agar or maybe your sterilization process.  when you say the jars looked fine for a week and then got 'wet' makes me think it could be endo-spores in the grain.do you have pics of the myc?  what times/psi do you sterilize at?


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: TedsDead]
    #26003180 -

Here are the plates. Noob condensation prevented pictures of plates 1 and 2 from the top. Tomentose sections never sporulated, so I assume it's cube Myc. I knew the risk with these plates, but I through them in grain anyway. Plate three I just threw the very tiny sections on the left in the grain. The others I threw in quarter plates. I've been cleaning the plates in the meantime, so I got nice looking backups ready to go.

plate 1, 2, 3


Plate 3 top, angled



PCed grains for 2 hours at >15 PSI with the initial 10 minute vent. Condensation started basically right before full colonization.


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First Grow

Edited by Raccoon (05/20/19 01:26 PM)

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: GoodVibrations]
    #26003186 -

Jars with bacteria should be spawned separately.


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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Re: What does it mean when a couple kernels of grain don't fully colonize? [Re: The Mycologist]
    #26006437 -

just keep dropping wedges onto grain until u get clean looking spawn. try lots of new plates too and make some "blank" test jars  so u know your grain isn't the issue


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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