Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Taking a biopsy for cloning
    #25915258 -

Can’t seem to reliably/consistently get a piece of tissue from inner stipe with a scalpel. Seems to have a tendency to come off in strips or more often not grab anything at all. Even tried an old syringe. I practiced taking a biopsy without putting it to agar a bit and just tossed it all. Should I just practice more ? I considered using a pair of tweezers or just dropping an entire pin from a shoebox on a plate. Obviously it would be highly contaminated.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Neowynd8] * 1
    #25915279 -

Rather than cut at it, lightly scrape it with the very tip of the scalpel. Failing that, I used a flame sterilised sewing needle the first time I tried to clone from the stem and that worked perfectly.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: UncleHoyphae]
    #25915282 -

You only really need a small part of tissue. If you can see it, it's plenty enough.


--------------------
How I do glass dishes

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: poisoned]
    #25915301 -

I tried scraping. Even cut the tip of one of my scalpel so it was flat instead of pointed. I actually inoculated a plate hoping a microscopic piece of tissue would be transferred. I also inoculated 4 plates with an older ms syringe with no growth on any. I started to suspect the agar and/or syringe.

Is dropping a pin from a shoebox a waste of time or would it be able to outrun the contamination?

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Neowynd8]
    #25915311 -

When I do it, I tear the mushroom in half, I then cut two slices the length of the stem on both sides.

———————————
-  -  -  -  -  -  -  -  -  -  -  -  - 
-  -  -  -  -  -  -  -  -  -  -  -  - 
———————————

and I cut those two slices at an angle, so they meet in the middle and cut a 3D triangle shaped wedge. 
——
| \  / |
|  \/  |
——

I slice that wedge like a loaf of bread, and then stab the center of the individual slices to transfer.


--------------------

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Ep1429]
    #25915336 -

Most of my fruits were small and hard to work with. I did get some larger ones on second flush but already dehydrated them. I was going to take a break from cultivating so just put the shoebox outside and it’s got a few small fruits now. Later I decided it would be best to at least have a culture on a plate for later use instead of restarting from me. I might try to work with some small fruits again.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Neowynd8]
    #25915345 -

Should be very simple. Harvest the mushroom, put into SAB..  sterilize hands/gloves... tear the mushroom in half.. and then just grab tissue from the center. I like to just barely cut a litle small square (not all the way through) and then kind of scoop out the outline with the tip of the scalpel.. and then wipe it onto a plate.

Works like a charm


--------------------
:greyalien:



Edited by Vibe_Enthusiast (04/04/19 11:13 AM)

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Vibe_Enthusiast]
    #25915348 -

I've always really wondered how long a culture will actually stay good on agar in a fridge.


--------------------
:greyalien:



Edited by Vibe_Enthusiast (04/04/19 11:14 AM)

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Vibe_Enthusiast]
    #25915407 -

I’ve got a six month old culture on a plate that is still viable.  I’ve read that they can last much longer, and I don’t doubt it. 

But I keep them fresh, just in case.  That six month old one is the best of the bunch, and while I have a slant of it, I’m still loathe to lose the original genetics.  Everything else is cycled out in two or three months.


--------------------

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Ep1429]
    #25915420 -

Ep1429 said:
I’ve got a six month old culture on a plate that is still viable.  I’ve read that they can last much longer, and I don’t doubt it. 

But I keep them fresh, just in case.  That six month old one is the best of the bunch, and while I have a slant of it, I’m still loathe to lose the original genetics.  Everything else is cycled out in two or three months.



That's good to know man! I'm finishing ch up these 9 jars and putting the hobby down for a bit. But would love to come back to these genetics!


--------------------
:greyalien:



Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Vibe_Enthusiast]
    #25915460 -

I've tried it all and have found that using an old scalpel blade (not one from a vintage shop, just one that's been heavily flamed a good number of times) really helps due to the carbon build up. So much so that if i'm about to take some tissue and the blade has recently been changed, I just flame the ever living shit out of it before i start. Then i scrape up the stipe until i see some peeling and then scrape down the stipe until said peeling comes off on my scalpel.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Ep1429]
    #25915742 -

Ep1429 said:
When I do it, I tear the mushroom in half, I then cut two slices the length of the stem on both sides.

———————————
-  -  -  -  -  -  -  -  -  -  -  -  - 
-  -  -  -  -  -  -  -  -  -  -  -  - 
———————————

and I cut those two slices at an angle, so they meet in the middle and cut a 3D triangle shaped wedge. 
——
| \  / |
|  \/  |
——

I slice that wedge like a loaf of bread, and then stab the center of the individual slices to transfer.



This works very well. +1 recommended.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Neowynd8]
    #25915779 -

Neowynd8 said:
Most of my fruits were small and hard to work with. I did get some larger ones on second flush but already dehydrated them. I was going to take a break from cultivating so just put the shoebox outside and it’s got a few small fruits now. Later I decided it would be best to at least have a culture on a plate for later use instead of restarting from me. I might try to work with some small fruits again.



If they're that small I would just find a little pin to clone and put the whole thing agar. The mycelium goes fast from them. You can transfer the new growth away from any contaminants.


--------------------

Edited by Kizzle (04/04/19 04:10 PM)

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Kizzle]
    #25916273 -

I’ve done that with pins from other plates but I thought they were relatively clean. Where as one from a shoebox which is currently sitting outside is dirty as it gets.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Neowynd8]
    #25916278 -

someone recommended to me doing it cob style, but I wasn't sure exactly what that means.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: woofwoof]
    #25916287 -

Never heard of it.

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: Neowynd8]
    #25916305 -

Cut a cub out in the stem that's easier(inside)

Extras: Filter Print Post Top
Re: Taking a biopsy for cloning [Re: woofwoof]
    #25916307 -

cronicr said:




Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy


Similar ThreadsPosterViewsRepliesLast post
* when cloning
( 1 2 all )
forevrgrounded98 3,715 26 11/06/03 01:14 AM
by Rose
* Mushroom cloning?? dimitri211 4,139 3 05/23/01 07:49 PM
by Anno
* Scalpel not turning red
( 1 2 3 4 all )
Shroombie2 2,413 64 05/18/21 09:31 AM
by ninja cat 09
* cloning...why won't it work for me? carbonhoots 1,507 9 02/14/02 11:47 PM
by Anno
* Want to try cloning.......think this would work? Numba9 1,316 8 09/23/03 05:45 AM
by downforpot
* Next step after birthing BRF cakes --> shred to bulk shoebox tek bugboy91 5,238 9 08/12/22 07:46 AM
by Gushroom
* Cloning on a low budget, how's this sound... QuitDrinking 1,269 11 12/25/02 05:49 PM
by Champion des Champignons
* Alcohol-sprayed glovebox: how to sterilize scalpel? vzalkaloid 4,235 3 07/14/03 01:14 PM
by vzalkaloid

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
943 topic views. 17 members, 177 guests and 86 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.007 seconds on 14 queries.