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Offlineforevrgrounded98
Stranger

Registered: 10/25/03
Posts: 283
Last seen: 17 years, 1 month
Re: when cloning [Re: Rose]
    #2074285 - 11/05/03 06:01 PM (20 years, 4 months ago)

aw yea, nice and dumb, just like i like it :smile:  So is there a certain dilution of h202 i should use or just let it sit in a cup of h202 right from the bottle for a few hrs?


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"If I don't see ya before the end of this one, I'll meet ya in the next one and don't be late" - Jimi Hendrix

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Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 18 years, 11 months
Re: when cloning [Re: forevrgrounded98]
    #2074461 - 11/05/03 06:59 PM (20 years, 4 months ago)

I can ssee the benefit of growing on agar to find an isolate strain specifically focused on the rhizomorphic are which is more likely to fruit. A cloned fruit body is guaranteed to be a fruiting strain since it has already been tested in that current grow. hormones sounds right to me too although I have seen projects with cloned fruitbodies where they were first grown on cakes using a multi-spore innoc the grown again under the same parameters with a much higher yeild low abhort rate and the fruitbodies that grew were all relatively similar in size. What would account for this? Does anyone know the benefit of growing an isolate out on agar compared with cloning a single fruitbody?


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OfflineRoseM
Devil's Advocate
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Registered: 09/24/03
Posts: 22,518
Loc: Mod not God Flag
Last seen: 1 year, 7 months
Trusted Cultivator
Re: when cloning [Re: thisone]
    #2075236 - 11/05/03 10:03 PM (20 years, 4 months ago)

I'd try that first forevr... but 10/1 Water/h2o2 is a common measurement.

I realy doubt pure h2o2 would harm the mycelia enough to kill it all.


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Fiddlesticks.


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InvisibleShamanorrhea
Stranger
Registered: 06/20/03
Posts: 304
Re: when cloning [Re: Rose]
    #2075852 - 11/06/03 12:59 AM (20 years, 4 months ago)

Cervantes, pure peroxide can be detrimental to mycelial growth. However, the mycelia will usually recover and begin to flourish after the initial shock of being exposed to peroxide.

There is a link on this site for a cloning procedure. I'm not too sure if it is useful or not but I'll post it anyways.

http://mv.lycaeum.org/mu/_liquid_culture.html

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OfflineRoseM
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Registered: 09/24/03
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Re: when cloning [Re: Shamanorrhea]
    #2075888 - 11/06/03 01:11 AM (20 years, 4 months ago)

Yeah I know that, but if you are taking a piece of shroom, I think the myc has a real fighting chance. I always hear h2o2 is detrimental to growth bu,t I've never heard it kills mycelia outright. It will stun the myc and a 10/1 water ratio... or even 2/1 would be better... but since we're talking about the lazy way, I think pure h2o2 is the simplest way to insure sterility and hydration of the mycelia.

I'm not saying it would be the first thing I'd try... I just like lazy tek theory. :smile: The more h2o2 you can use... the less need for a flowhood. A lazy person has no use for a high tek contraption like a flowhood or a glovebox! Those take work!

Cool link.



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Fiddlesticks.


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OfflineTrippinSpinners
Mr. Nice Guy

Registered: 10/14/03
Posts: 305
Last seen: 18 years, 4 months
Re: when cloning [Re: forevrgrounded98]
    #2075893 - 11/06/03 01:13 AM (20 years, 4 months ago)



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OfflineRoseM
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Registered: 09/24/03
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Re: when cloning [Re: TrippinSpinners]
    #2075896 - 11/06/03 01:14 AM (20 years, 4 months ago)

Looks fun.


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Fiddlesticks.


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