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OfflineNumba9
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Want to try cloning.......think this would work?
    #1144411 - 12/16/02 04:40 AM (18 years, 11 months ago)

I`ve seen the teks on taking a good specimen and slicing the skin back and removing flesh from inside the shroom. Do you guys think If i took my small coffee grinder and wiped the inside down with alcohol .replaced the lid......  then did the flesh transfer into the coffee grinder and added sterile water from a syringe and fired that sucker up and made a slurry . Then sucked that solution up in another sterile syringe. It seems that would be some great innoculation cloned mycelia and not have to wait for multi-spores to germinate. Some thoughts from the pros would be appreciated. Thanks in advance :smile:
 


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OfflineWaldarbeiter
can you eat it?

Registered: 03/07/02
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Re: Want to try cloning.......think this would work? [Re: Numba9]
    #1144472 - 12/16/02 05:17 AM (18 years, 11 months ago)

hi!
wouldn't o it this way,
1.) you would destroy too many cells if you did so
2.)how to get rid of the alcohol? it may kill the cells

easy way: the known way.
take a clean knife, and put a small tissue sample directly to your jar.
after this jar is colonized , you can make lotsof jar-to-jar transfers.




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OfflineRaadt
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Re: Want to try cloning.......think this would work? [Re: Numba9]
    #1144553 - 12/16/02 05:59 AM (18 years, 11 months ago)

Personally, I suggest taking a tek and following it exactly. Otherwise you're begging for problems.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Anonymous

Post deleted by Administrator [Re: Numba9]
    #1145877 - 12/16/02 01:46 PM (18 years, 11 months ago)



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Offlinepsychomycul
Praise me for Iam great

Registered: 03/21/02
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Re: Want to try cloning.......think this would wor [Re: ]
    #1932628 - 09/19/03 03:40 PM (18 years, 2 months ago)

"
Personally, I suggest taking a tek and following it exactly. Otherwise you're begging for problems.
"
What? How could you say that. I guess I can agree if the cloning is done by an inexperienced individual, but isn't that the nature of progression. Trying new things and hoping for the best. Only one Tek for everything would exist if everyone stuck to the Teks.


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Offlinewalk
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Registered: 06/22/03
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Re: Want to try cloning.......think this would wor [Re: psychomycul]
    #1933080 - 09/19/03 06:16 PM (18 years, 2 months ago)

If you just want it to work use a tek.
You want to do something interesting then experiment.
I agree with both of you.


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OfflineRogerRabbitM
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Re: Want to try cloning.......think this would wor [Re: walk]
    #1934091 - 09/20/03 01:47 AM (18 years, 2 months ago)

First of all, alcohol wouldn't sterilize a coffee grinder. If you want to grind up a mushie, you need an eberbach blender, fresh from the pc with the sterile water pc'd with it. If you want to clone a mushroom, simply tear it in half lengthwise, then flame sterilize a scalpel and cut a small (no bigger than a grain of rice) piece out of it, from deep in the center of the fruitbody, and place on agar. It makes no difference whether you take a sample from the cap or stem. I've done both in side by side tests, and they both work. Experimenting is great and it's how we learn. But you don't have to invent the wheel all over again. Learn from those who have made the mistakes in the past.


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Invisiblemycofile
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Re: Want to try cloning.......think this would wor [Re: RogerRabbit]
    #1941047 - 09/22/03 04:12 PM (18 years, 2 months ago)

Look for either "shroomdadi's mycomachine" or the "blender usage" section in "9er's tek".

You got the general idea right, but these two resources will set you straight on the specifics.


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"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisibledownforpot
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Registered: 06/25/01
Posts: 5,715
Re: Want to try cloning.......think this would wor [Re: mycofile]
    #1943565 - 09/23/03 07:45 AM (18 years, 2 months ago)

Just search for my post about using honey water to clone mushrooms.


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