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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: Mushroom-Hut Mono Tub Substrate, Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale

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Agar plate bacteria contam?
    #25543743 -



I had this lc that keept giving me lottery contams, so i tested it on agar. This is how it looks. Contamination?

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25544025 -

Anyone?

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25544073 -

Lottery contams?


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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Re: Agar plate bacteria contam? [Re: VroomerMcZoomers]
    #25544127 -

VroomerMcZoomers said:
Lottery contams?


I meant my jars keep getting bacterial. Majority of them. Some ok but more bacterial. I have used whole oats and they get extremely packed and hard you can’t shake them. Some smell sweet.
Looks like I’m injecting contams but not sure.

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25544158 -

How much did you shoot on there? If it was just a drop or two in the middle contam for sure.


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Re: Agar plate bacteria contam? [Re: sandman420]
    #25544307 -

sandman420 said:
How much did you shoot on there? If it was just a drop or two in the middle contam for sure.


Several drops :frown:
Looks funky in the middle, i did take out a piece and placed it on another plate.

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25554705 -


Did one transfer from the plate, this is how it looks. Is this mycelium?

Edited by Booooooooom (10/21/18 07:03 AM)

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25554737 -

Unless your agar recipe is really really weak in nutes that does not look like cubensis mycelium.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Agar plate bacteria contam? [Re: sandman420]
    #25554969 -

sandman420 said:
Unless your agar recipe is really really weak in nutes that does not look like cubensis mycelium.


I dont know, i used honey agar 4% to 100ml water. Maybe the honey is weak in sugar. How can i know for sure if its weak mycelium/contamination?

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25554975 -

That’s crap media and likely a big part of your problem.  Nutes and agar should be used at 2% each.  Try dog food if you can’t get mea or look up pda.


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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25554980 -

Kinda hard to tell with the condensation but it looks ok maybe do more transfers. next time try putting the dish over led when taking a pic. Is it really white, yellowish, or grey?


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"The task we face today is to
understand the language of nature"

~Paul Stamets~

Edited by ExoMyco (10/21/18 09:38 AM)

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Re: Agar plate bacteria contam? [Re: ExoMyco]
    #25554987 -

No wonder your agar looks like that, use a real agar recipe like MEA or PDA or DFA. Problem solved.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Agar plate bacteria contam? [Re: Booooooooom]
    #25555016 -

Booooooooom said:
I dont know, i used honey agar 4% to 100ml water. Maybe the honey is weak in sugar. How can i know for sure if its weak mycelium/contamination?


What if I told you that honey has antifungal properties.
:blueorred:

But seriously, you should look into light malt extract...2%


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Re: Agar plate bacteria contam? [Re: Tormato]
    #25559729 -


This is how it looked today when i took transfers to new agar plates with light malt extract.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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