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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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First time agar attempt. How’s this sound to you?
    #25554168 -

Ordered premixed bag of MEA agar. Have half pint wide mouth jars. I have synthetic filter discs on hand already. Make my agar per directions on bag. Pour agar into my jars with a quarter inch hole and synthetic filter disk. Pressure cook for 20-30 mins. Sounds too easy. What am I missing and where am I fucking up?

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Re: First time agar attempt. How’s this sound to you? [Re: HuckleBerrySlim]
    #25554360 -

That's about it. 

You are going to find that you wish you were working with petris once the depth of those jars starts fucking with your scalpel's dexterity,  but it'll be OK.

Making it from scratch is just as easy as a premix, and if you want to keep going on and on with transfers to new plates, it is said you should change the nutrient source each time to keep the myc vibrant, so, you may want to keep that in mind.


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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Re: First time agar attempt. How’s this sound to you? [Re: VroomerMcZoomers]
    #25554364 -

I just made this Agar Tek yesterday

Mateah said:
Making Agar is really simple, as I will demonstrate in this post.


For every 100ml water I use 2g Agar Agar, and 2g of nutrients.
In this Tek I'm making 300ml DFA and 300ml MEA.


Let's make DFA:
I measure out 6g of Agar Agar and 6g of dog food that I grind to powder.

Stir everything down into 300ml of boiling water and keep stirring until there are no more clumps.


When the mix is finished I pour into these handsome bottles and wrap with alu-foil on top. No caps.

Left side DFA right side MEA


I PC some scalpels and water with this as well that I will be using later for cake inoculate.

20 min at 15 PSI or higher.


As soon as PC is ready to be opened I take the bottles directly from the PC into the SAB.

Bottles rest in a water bath inside qt jars. A meat thermometer reads water temps. At 45-46C I go to work.


This is how it looks when plates are being poured

Anyone can do this
:bongload:




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Cakes inside Water Tub with optimal Surface Conditions

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Re: First time agar attempt. How’s this sound to you? [Re: VroomerMcZoomers]
    #25554511 -

VroomerMcZoomers said:
it is said you should change the nutrient source each time to keep the myc vibrant, so, you may want to keep that in mind.




That's interesting, I've not heard that before but I've noticed it going from tater water to grain water agar the myc takes to it very rapidly, but never made the conneaction before.


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"great things may come to those who wait, but only what's left by those who hustle"

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Re: First time agar attempt. How’s this sound to you? [Re: Bumholio]
    #25555114 -

It's supposed to delay senescence...


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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