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Amazon Shop for: Agar, Malt Extract, pH Test Strips

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OfflineTheMessenger
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Registered: 05/04/03
Posts: 1
Last seen: 13 years, 6 months
Malt and agar dosent react after 12 days !!!
    #1519163 - 05/04/03 11:54 AM (13 years, 6 months ago)

i have baught and pre made media from a laboratories there was the content

FORMULA
in grams per liter purified filtered water

Gelatin Peptone 0,78

Maltose 12,75

Dextrin 2,75

Glycerol 2,35

Agar 15,0

pH 4,6 +/- 0,2 ? 25? C



and after 10 days notin absolutly notin happen no mold no contamination no mycelium growin !!! i seal my plate put in a box at 25 celius (80F)
notin ! whats wrong with that is it my spore which is crap or my agar mal premixed media!? the spore have been bought into a local store and i think its trustable and the premixed media have been bought from a pro lab
anyway thanks !


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OfflineMykeOfile
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Registered: 04/30/03
Posts: 212
Loc: behind the bookshelf
Last seen: 12 years, 9 months
Re: Malt and agar dosent react after 12 days !!! [Re: TheMessenger]
    #1519344 - 05/04/03 02:04 PM (13 years, 6 months ago)

yeah, one or both of those isnt worth a damn, my guess since u have no contams either is that it is u'r agar, u might test that by swabing a dirty counter top or in between u'r toes and seeing if ne thing grows


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 191,500
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Malt and agar dosent react after 12 days !!! [Re: MykeOfile]
    #1521043 - 05/05/03 03:02 AM (13 years, 6 months ago)

keep in mind that many of the premix agars are geared for specific bacteria/molds/fungi...I would do without the peptone and glycerol, malt extract, dextrose and agar are all you need for the spores to germinate and with 4.6 ph, I really not suprised it didnt contaminate...most things living or organic dont care for acid....except for LSD....we do care fo that.....

if you need some agar...premix or powdered lemme know...I have 10 kilos I'm waiting for packaging on....I already have some MEA mixed you and also have some TSA on hand....I can also prep it so all you have to do is heat and pour

P#1


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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